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Middle East Respiratory Syndrome Coronavirus Efficiently Infects Human Primary T Lymphocytes and Activates the Extrinsic and Intrinsic Apoptosis Pathways
Open accessAbstract
Middle East respiratory syndrome (MERS) is associated with a mortality rate of >35%. We previously showed that MERS coronavirus (MERS-CoV) could infect human macrophages and dendritic cells and induce cytokine dysregulation. Here, we further investigated the interplay between human primary T cells and MERS-CoV in disease pathogenesis. Importantly, our results suggested that MERS-CoV efficiently infected T cells from the peripheral blood and from human lymphoid organs, including the spleen and the tonsil. We further demonstrated that MERS-CoV infection induced apoptosis in T cells, which involved the activation of both the extrinsic and intrinsic apoptosis pathways. Remarkably, immunostaining of spleen sections from MERS-CoV-infected common marmosets demonstrated the presence of viral nucleoprotein in their CD3(+) T cells. Overall, our results suggested that the unusual capacity of MERS-CoV to infect T cells and induce apoptosis might partly contribute to the high pathogenicity of the virus.
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“Infection of EC through infected PBMC has been documented for members of several virus families, including bunya, herpes, filo, flavi, toga, and arena viruses (Charrel and de Lamballerie, 2010; Chu et al., 2016; Gowen and Hickerson, 2017; Kunz and de la Torre, 2017; Ma et al., 2013; Mackow and Gavrilovskaya, 2009).”
From Equid Herpesvirus-1 Exploits the Extracellular Matrix of Mononuclear Cells to Ensure Transport to Target Cells (2020) · cites this paper as Chu et al., 2016
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Siu, K., Yeung, M. L., Kok, K., Yuen, K., Kew, C., Lui, P., Chan, C., Tse, H., Woo, P. C. Y., Yuen, K., et al. (2014) · Journal of Virology · doi:10.1128/jvi.03649-13 · Cited by 179
Self-citationRetractedApoptosis: A Review of Programmed Cell Death
Elmore, S. (2007) · Toxicologic Pathology · doi:10.1080/01926230701320337 · Cited by 13,853
Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation.
Gavrieli, Y., Sherman, Y., & Ben-Sasson, S. A. (1992) · The Journal of cell biology · doi:10.1083/jcb.119.3.493 · Cited by 9,444
Isolation of a Novel Coronavirus from a Man with Pneumonia in Saudi Arabia
Zaki, A. M., van Boheemen, S., Bestebroer, T. M., Osterhaus, A. D. M. E., & Fouchier, R. A. M. (2012) · New England Journal of Medicine · doi:10.1056/nejmoa1211721 · Cited by 5,677
Identification of a novel coronavirus in patients with severe acute respiratory syndrome
Drosten, C., Günther, S., Preiser, W., van der Werf, S., Brodt, H.-R., Becker, S., Rabenau, H., Panning, M., Kolesnikova, L., Fouchier, R. A. M., et al. (2003) · New England Journal of Medicine · doi:10.1056/nejmoa030747 · Cited by 4,574
Coronavirus as a possible cause of severe acute respiratory syndrome
Peiris, M. J. S., Lai, S., Poon, L. L. M., Guan, Y., Yam, L., Lim, W., Nicholls, J. M., Yee, W., Yan, W., Cheung, M., et al. (2003) · The Lancet · doi:10.1016/s0140-6736(03)13077-2 · Cited by 2,916
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Raj, V. S., Mou, H., Smits, S. L., Dekkers, D. H. W., Müller, M. A., Dijkman, R., Muth, D., Demmers, J. A. A., Zaki, A., Fouchier, R. A. M., et al. (2013) · Nature · doi:10.1038/nature12005 · Cited by 2,061
Assiri, A., Al-Tawfiq, J. A., Al-Rabeeah, A. A., Al-Rabiah, F. A., Al-Hajjar, S., Al-Barrak, A., Flemban, H., Al-Nassir, W. N., Balkhy, H. H., Al-Hakeem, R. F., et al. (2013) · The Lancet Infectious Diseases · doi:10.1016/s1473-3099(13)70204-4 · Cited by 1,435
Cell death by pyroptosis drives CD4 T-cell depletion in HIV-1 infection
Doitsh, G., Galloway, N. L. K., Geng, X., Yang, Z., Monroe, K. M., Zepeda, O., Hunt, P. W., Hatano, H., Sowinski, S., Muñoz-Arias, I., et al. (2013) · Nature · doi:10.1038/nature12940 · Cited by 1,216
Severe acute respiratory syndrome coronavirus as an agent of emerging and reemerging infection
Cheng, V. C. C., Lau, S. K. P., Woo, P. C. Y., & Yuen, K. Y. (2007) · Clinical Microbiology Reviews · doi:10.1128/cmr.00023-07 · Cited by 1,192
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Clinical and immunological features of severe and moderate coronavirus disease 2019
Chen, G., Wu, D., Guo, W., Cao, Y., Huang, D., Wang, H., Wang, T., Zhang, X., Chen, H., Yu, H., et al. (2020) · Journal of Clinical Investigation · doi:10.1172/jci137244 · Cited by 4,750
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Gupta, A., Madhavan, M. V., Sehgal, K., Nair, N., Mahajan, S., Sehrawat, T. S., Bikdeli, B., Ahluwalia, N., Ausiello, J. C., Wan, E. Y., et al. (2020) · Nature Medicine · doi:10.1038/s41591-020-0968-3 · Cited by 3,185
Channappanavar, R., & Perlman, S. (2017) · Seminars in Immunopathology · doi:10.1007/s00281-017-0629-x · Cited by 2,524
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Metadata
- Authors
- Chu, H., Zhou, J., Wong, B. H.-Y., Li, C., Chan, J. F.-W., Cheng, Z.-S., Yang, D., Wang, D., Lee, A. C.-Y., Li, C., Yeung, M.-L., Cai, J.-P., Chan, I. H.-Y., Ho, W.-K., To, K. K.-W., Zheng, B.-J., Yao, Y., Qin, C., & Yuen, K.-Y.
- Affiliations
- University of Hong Kong (HK)
- Chinese Academy of Medical Sciences & Peking Union Medical College (CN)
- Zhejiang University (CN)
- Corresponding author
- Chu, Hin
- ORCID
- Venue
- Journal of Infectious Diseases
- Publisher
- Oxford University Press (OUP)
- Published
- 2015-07-22
- Volume
- 213
- Issue
- 6
- Pages
- 904-914
- ISSN
- 0022-1899 (ISSN-L)
- 1537-6613
- PMC
- PMC7107330
- OpenAlex
- W1947409115
- Cited by
- 448
- References
- 50
- Citations by year
- 2026: 4 · 2025: 16 · 2024: 19 · 2023: 22 · 2022: 50 · 2021: 117 · 2020: 149 · 2019: 14 · 2018: 15 · 2017: 12 · 2016: 14 · 2015: 4
- FWCI
- 11.68
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- The Journal of Infectious Diseases (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- The HKU Scholars Hub (University of Hong Kong) (submittedVersion)
- HighWire Press Open Archive (submittedVersion)
- Funders
- Research Grants Council of the Hong Kong Special Administrative Region (T11/707/15)
- Providence Foundation Limited
- University of Hong Kong Small Project Funding (201409176024)
- Hong Kong Health and Medical Research Fund (14131392)
- Hong Kong Research Grants Council (N_HKU728/14)
- National Science and Technology Major Projects of Infectious Disease (2012ZX10004501-004)
- Topics
- Health Sciences › Medicine › Infectious Diseases › SARS-CoV-2 and COVID-19 Research
- Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Virus Infections Studies
- Health Sciences › Medicine › Infectious Diseases › Viral Infections and Outbreaks Research
- Keywords
- MERS-CoV, T cells, T cell infection, apoptosis, extrinsic apoptotic pathway, mitochondrial apoptosis, spleen, tonsil, marmoset, viral pathogenicity, lymphoid organs
- MeSH terms
- Animals, Antibodies, Viral, Callithrix, Cells, Cultured, Gene Expression Regulation, Humans, Spleen, T-Lymphocytes, Palatine Tonsil, Apoptosis, Dipeptidyl Peptidase 4, Severe acute respiratory syndrome-related coronavirus, Middle East Respiratory Syndrome Coronavirus
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