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Middle East Respiratory Syndrome Coronavirus Efficiently Infects Human Primary T Lymphocytes and Activates the Extrinsic and Intrinsic Apoptosis Pathways
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Middle East respiratory syndrome (MERS) is associated with a mortality rate of >35%. We previously showed that MERS coronavirus (MERS-CoV) could infect human macrophages and dendritic cells and induce cytokine dysregulation. Here, we further investigated the interplay between human primary T cells and MERS-CoV in disease pathogenesis. Importantly, our results suggested that MERS-CoV efficiently infected T cells from the peripheral blood and from human lymphoid organs, including the spleen and the tonsil. We further demonstrated that MERS-CoV infection induced apoptosis in T cells, which involved the activation of both the extrinsic and intrinsic apoptosis pathways. Remarkably, immunostaining of spleen sections from MERS-CoV-infected common marmosets demonstrated the presence of viral nucleoprotein in their CD3(+) T cells. Overall, our results suggested that the unusual capacity of MERS-CoV to infect T cells and induce apoptosis might partly contribute to the high pathogenicity of the virus.
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“Infection of EC through infected PBMC has been documented for members of several virus families, including bunya, herpes, filo, flavi, toga, and arena viruses (Charrel and de Lamballerie, 2010; Chu et al., 2016; Gowen and Hickerson, 2017; Kunz and de la Torre, 2017; Ma et al., 2013; Mackow and Gavrilovskaya, 2009).”
From Equid Herpesvirus-1 Exploits the Extracellular Matrix of Mononuclear Cells to Ensure Transport to Target Cells (2020) · cites this paper as Chu et al., 2016
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Metadata
- Authors
- Chu, H., Zhou, J., Wong, B. H.-Y., Li, C., Chan, J. F.-W., Cheng, Z.-S., Yang, D., Wang, D., Lee, A. C.-Y., Li, C., Yeung, M.-L., Cai, J.-P., Chan, I. H.-Y., Ho, W.-K., To, K. K.-W., Zheng, B.-J., Yao, Y., Qin, C., & Yuen, K.-Y.
- Affiliations
- University of Hong Kong (HK)
- Chinese Academy of Medical Sciences & Peking Union Medical College (CN)
- Zhejiang University (CN)
- Corresponding author
- Chu, Hin
- ORCID
- Venue
- Journal of Infectious Diseases
- Publisher
- Oxford University Press (OUP)
- Published
- 2015-07-22
- Volume
- 213
- Issue
- 6
- Pages
- 904-914
- ISSN
- 0022-1899 (ISSN-L)
- 1537-6613
- PMC
- PMC7107330
- OpenAlex
- W1947409115
- Cited by
- 448
- References
- 50
- Citations by year
- 2026: 4 · 2025: 16 · 2024: 19 · 2023: 22 · 2022: 50 · 2021: 117 · 2020: 149 · 2019: 14 · 2018: 15 · 2017: 12 · 2016: 14 · 2015: 4
- FWCI
- 11.68
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- The Journal of Infectious Diseases (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- The HKU Scholars Hub (University of Hong Kong) (submittedVersion)
- HighWire Press Open Archive (submittedVersion)
- Funders
- Research Grants Council of the Hong Kong Special Administrative Region (T11/707/15)
- Providence Foundation Limited
- University of Hong Kong Small Project Funding (201409176024)
- Hong Kong Health and Medical Research Fund (14131392)
- Hong Kong Research Grants Council (N_HKU728/14)
- National Science and Technology Major Projects of Infectious Disease (2012ZX10004501-004)
- Topics
- Health Sciences › Medicine › Infectious Diseases › SARS-CoV-2 and COVID-19 Research
- Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Virus Infections Studies
- Health Sciences › Medicine › Infectious Diseases › Viral Infections and Outbreaks Research
- Keywords
- MERS-CoV, T cells, T cell infection, apoptosis, extrinsic apoptotic pathway, mitochondrial apoptosis, spleen, tonsil, marmoset, viral pathogenicity, lymphoid organs
- MeSH terms
- Animals, Antibodies, Viral, Callithrix, Cells, Cultured, Gene Expression Regulation, Humans, Spleen, T-Lymphocytes, Palatine Tonsil, Apoptosis, Dipeptidyl Peptidase 4, Severe acute respiratory syndrome-related coronavirus, Middle East Respiratory Syndrome Coronavirus
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