Protective Efficacy of Nucleic Acid Vaccines Against Transmission of Zika Virus During Pregnancy in Mice
Open accessAbstract
Zika virus (ZIKV) caused an epidemic of congenital malformations in 2015–2016. Although many vaccine candidates have been generated, few have demonstrated efficacy against congenital ZIKV infection. Here, we evaluated lipid-encapsulated messenger RNA (mRNA) vaccines and a DNA plasmid vaccine encoding the prM-E genes of ZIKV in mouse models of congenital infection. Although the DNA vaccine provided comparable efficacy against vertical transmission of ZIKV, the mRNA vaccines, including one that minimizes antibody-dependent enhancement of infection, elicited higher levels of antigen-specific long-lived plasma cells and memory B cells. Despite the induction of robust neutralizing antibody titers by all vaccines, breakthrough seeding of the placenta and fetal head was observed in a small subset of type I interferon signaling–deficient immunocompromised dams. In comparison, evaluation of one of the mRNA vaccines in a human STAT2-knockin transgenic immunocompetent mouse showed complete protection against congenital ZIKV transmission. These data will inform ongoing human ZIKV vaccine development efforts and enhance our understanding of the correlates of vaccine-induced protection.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In several studies, maternal vaccination with mRNA-loaded LNPs prevented fetal Zika virus transmission in pregnant mice.
“In several studies, maternal vaccination with mRNA-loaded LNPs prevented fetal Zika virus transmission in pregnant mice225,226 and protected mouse neonates from herpesvirus227 and group A and group B streptococci228.”
From mRNA vaccines for infectious diseases: principles, delivery and clinical translation (2021) · cites this paper as [226]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
1 retracted
RETRACTED ARTICLE: DCs induce CD40-independent immunoglobulin class switching through BLyS and APRIL
Litinskiy, M. B., Nardelli, B., Hilbert, D. M., He, B., Schaffer, A., Casali, P., & Cerutti, A. (2002) · Nature Immunology · doi:10.1038/ni829 · Cited by 1,252 · see citing works
RetractedmRNA vaccines — a new era in vaccinology
Pardi, N., Hogan, M. J., Porter, F. W., & Weissman, D. (2018) · Nature Reviews Drug Discovery · doi:10.1038/nrd.2017.243 · Cited by 4,667 · see citing works
Lanciotti, R. S., Kosoy, O. L., Laven, J. J., Velez, J. O., Lambert, A. J., Johnson, A. J., Stanfield, S. M., & Duffy, M. R. (2008) · Emerging infectious diseases · doi:10.3201/eid1408.080287 · Cited by 2,173 · see citing works
Characterizing the Pattern of Anomalies in Congenital Zika Syndrome for Pediatric Clinicians
Moore, C. A., Staples, J. E., Dobyns, W. B., Pessoa, A. L. S., Ventura, C. V. O. C., da Fonseca, E. B., Ribeiro, E. M., Ventura, L. O., Neto, N. N., Arena, J. F., et al. (2016) · Archives of Pediatrics and Adolescent Medicine · doi:10.1001/jamapediatrics.2016.3982 · Cited by 953 · see citing works
A Mouse Model of Zika Virus Pathogenesis
Lazear, H. M., Govero, J., Smith, A. M., Platt, D. J., Fernandez, E., Miner, J. J., & Diamond, M. S. (2016) · Cell Host & Microbe · doi:10.1016/j.chom.2016.03.010 · Cited by 909 · see citing works
Self-citationModified mRNA vaccines protect against zika virus infection
Richner, J. M., Himansu, S., Dowd, K. A., Butler, S. L., Salazar, V., Fox, J. M., Julander, J. G., Tang, W. W., Shresta, S., Pierson, T. C., et al. (2017) · Cell · doi:10.1016/j.cell.2017.02.017 · Cited by 909 · see citing works
Self-citationZika Virus Infection during Pregnancy in Mice Causes Placental Damage and Fetal Demise
Miner, J. J., Cao, B., Govero, J., Smith, A. M., Fernández, E., Cabrera, O. H., Garber, C., Elam-Noll, M., Klein, R. S., Noguchi, K. K., et al. (2016) · Cell · doi:10.1016/j.cell.2016.05.008 · Cited by 817 · see citing works
Self-citationZika Virus Targets Human STAT2 to Inhibit Type I Interferon Signaling
Grant, A., Ponia, S. S., Tripathi, S., Balasubramaniam, V. R. M. T., Miorin, L., Sourisseau, M., Schwarz, M. C., Sánchez‐Seco, M. P., Evans, M. J., Best, S. M., et al. (2016) · Cell Host & Microbe · doi:10.1016/j.chom.2016.05.009 · Cited by 789 · see citing works
Immune responses at the maternal-fetal interface
Ander, S. E., Diamond, M., & Coyne, C. B. (2019) · Science Immunology · doi:10.1126/sciimmunol.aat6114 · Cited by 660 · see citing works
Self-citationRegulation of antiviral T cell responses by type I interferons
Crouse, J., Kalinke, U., & Oxenius, A. (2015) · Nature reviews. Immunology · doi:10.1038/nri3806 · Cited by 480 · see citing works
Davis, B. S., Chang, G. J., Cropp, B. C., Roehrig, J. T., Martin, D. A., Mitchell, C. J., Bowen, R. A., & Bunning, M. L. (2001) · Journal of Virology · doi:10.1128/jvi.75.9.4040-4047.2001 · Cited by 447 · see citing works
Lethal Antibody Enhancement of Dengue Disease in Mice Is Prevented by Fc Modification
Balsitis, S. J., Williams, K. L., Lachica, R., Flores, D., Kyle, J. L., Mehlhop, E., Johnson, S., Diamond, M., Beatty, P. R., & Harris, E. (2010) · PLoS Pathogens · doi:10.1371/journal.ppat.1000790 · Cited by 412 · see citing works
Self-citationRapid development of a DNA vaccine for Zika virus
Dowd, K. A., Ko, S., Morabito, K. M., Yang, E. S., Pelc, R. S., DeMaso, C. R., Castilho, L. R., Abbink, P., Boyd, M. B., Nityanandam, R., et al. (2016) · Science · doi:10.1126/science.aai9137 · Cited by 396 · see citing works
Self-citationThe emergence of Zika virus and its new clinical syndromes
Pierson, T. C., & Diamond, M. (2018) · Nature · doi:10.1038/s41586-018-0446-y · Cited by 382 · see citing works
Self-citationBon, A. L., Thompson, C., Kamphuis, E., Durand, V., Rossmann, C., Kalinke, U., & Tough, D. F. (2006) · The Journal of Immunology · doi:10.4049/jimmunol.176.4.2074 · Cited by 361 · see citing works
Zellweger, R. M., Prestwood, T. R., & Shresta, S. (2010) · Cell Host & Microbe · doi:10.1016/j.chom.2010.01.004 · Cited by 340 · see citing works
Oliphant, T., Nybakken, G. E., Engle, M. J., et al. (2006) · Journal of Virology · doi:10.1128/jvi.01732-06 · Cited by 306 · see citing works
Zika virus inhibits type‐I interferon production and downstream signaling
Kumar, A., Hou, S., Airo, A. M., Limonta, D., Mancinelli, V., Branton, W. G., Power, C., & Hobman, T. C. (2016) · EMBO Reports · doi:10.15252/embr.201642627 · Cited by 305 · see citing works
Gaudinski, M. R., Houser, K. V., Morabito, K. M., Hu, Z., Yamshchikov, G. V., Rothwell, R. S., Berkowitz, N. M., Mendoza, F. H., Saunders, J. G., Novik, L., et al. (2017) · The Lancet · doi:10.1016/s0140-6736(17)33105-7 · Cited by 302 · see citing works
Self-citationEvaluation of candidate vaccine approaches for MERS-CoV
Wang, L., Shi, W., Joyce, M. G., Modjarrad, K., Zhang, Y., Leung, K., Lees, C. R., Zhou, T., Yassine, H. M., Kanekiyo, M., et al. (2015) · Nature Communications · doi:10.1038/ncomms8712 · Cited by 300 · see citing works
Self-citationVaccine Mediated Protection Against Zika Virus-Induced Congenital Disease
Richner, J. M., Jagger, B. W., Shan, C., Fontes, C. R., Dowd, K. A., Cao, B., Himansu, S., Caine, E. A., Nunes, B. T. D., Medeiros, D. B. A., et al. (2017) · Cell · doi:10.1016/j.cell.2017.06.040 · Cited by 267 · see citing works
Self-citationZika Virus Antagonizes Type I Interferon Responses during Infection of Human Dendritic Cells
Bowen, J. R., Quicke, K. M., Maddur, M. S., O’Neal, J. T., McDonald, C. E., Fedorova, N. B., Puri, V., Shabman, R. S., Pulendran, B., & Suthar, M. S. (2017) · PLoS Pathogens · doi:10.1371/journal.ppat.1006164 · Cited by 264 · see citing works
Sheehan, K. C. F., Lai, K. S., Dunn, G. P., Bruce, A. T., Diamond, M. S., Heutel, J. D., Dungo-Arthur, C., Carrero, J. A., White, J. M., Hertzog, P. J., et al. (2006) · Journal of Interferon & Cytokine Research · doi:10.1089/jir.2006.26.804 · Cited by 260 · see citing works
Self-citationImpact of preexisting dengue immunity on Zika virus emergence in a dengue endemic region
Rodríguez-Barraquer, I., Costa, F., Nascimento, E. J. M., Nery, N., Castanha, P. M. S., do Sacramento, G. A., Cruz, J. S., Carvalho, M., de Oliveira, D., Hagan, J. E., et al. (2019) · Science · doi:10.1126/science.aav6618 · Cited by 250 · see citing works
An Immunocompetent Mouse Model of Zika Virus Infection
Gorman, M. J., Caine, E. A., Zaitsev, K., Begley, M., Weger‐Lucarelli, J., Uccellini, M. B., Tripathi, S., Morrison, J. M., Yount, B. L., Dinnon, K. H., et al. (2018) · Cell Host & Microbe · doi:10.1016/j.chom.2018.04.003 · Cited by 240 · see citing works
Self-citationMapping and Role of the CD8 + T Cell Response During Primary Zika Virus Infection in Mice
Ngono, A. E., Vizcarra, E. A., Tang, W. W., Sheets, N., Joo, Y., Kim, K., Gorman, M. J., Diamond, M., & Shresta, S. (2017) · Cell Host & Microbe · doi:10.1016/j.chom.2016.12.010 · Cited by 237 · see citing works
Self-citationWeissman, D. (2015) · Expert Review of Vaccines · doi:10.1586/14760584.2015.973859 · Cited by 228 · see citing works
Broadly Neutralizing Activity of Zika Virus-Immune Sera Identifies a Single Viral Serotype
Dowd, K. A., DeMaso, C. R., Pelc, R. S., Speer, S. D., Smith, A. R. Y., Goo, L., Platt, D. J., Mascola, J. R., Graham, B. S., Mulligan, M. J., et al. (2016) · Cell Reports · doi:10.1016/j.celrep.2016.07.049 · Cited by 206 · see citing works
Self-citationDengvaxia sensitizes seronegatives to vaccine enhanced disease regardless of age
Halstead, S. B. (2017) · Vaccine · doi:10.1016/j.vaccine.2017.09.089 · Cited by 205 · see citing works
Highly efficient maternal-fetal Zika virus transmission in pregnant rhesus macaques
Nguyen, S. M., Antony, K. M., Dudley, D. M., Kohn, S., Simmons, H. A., Wolfe, B., Salamat, M. S., Teixeira, L. B. C., Wiepz, G. J., Thoong, T. H., et al. (2017) · PLoS Pathogens · doi:10.1371/journal.ppat.1006378 · Cited by 201 · see citing works
Ding, Q., Gaska, J. M., Douam, F., Wei, L., Kim, D., Balev, M., Heller, B. L., & Ploß, A. (2018) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1803406115 · Cited by 189 · see citing works
Prior dengue virus infection and risk of Zika: A pediatric cohort in Nicaragua
Gordon, A. L., Gresh, L., Ojeda, S., et al. (2019) · PLoS Medicine · doi:10.1371/journal.pmed.1002726 · Cited by 154 · see citing works
A single-dose live-attenuated vaccine prevents Zika virus pregnancy transmission and testis damage
Shan, C., Muruato, A. E., Jagger, B. W., et al. (2017) · Nature Communications · doi:10.1038/s41467-017-00737-8 · Cited by 143 · see citing works
Self-citationPrior Exposure to Zika Virus Significantly Enhances Peak Dengue-2 Viremia in Rhesus Macaques
George, J., Valiant, W. G., Mattapallil, M. J., et al. (2017) · Scientific Reports · doi:10.1038/s41598-017-10901-1 · Cited by 139 · see citing works
Khandia, R., Munjal, A. K., Dhama, K., Karthik, K., Tiwari, R., Malik, Y. S., Singh, R. K., & Chaicumpa, W. (2018) · Frontiers in Immunology · doi:10.3389/fimmu.2018.00597 · Cited by 135 · see citing works
Long-term circulation of Zika virus in Thailand: an observational study
Ruchusatsawat, K., Wongjaroen, P., Posanacharoen, A., Rodríguez-Barraquer, I., Sangkitporn, S., Cummings, D. A. T., & Salje, H. (2019) · The Lancet Infectious Diseases · doi:10.1016/s1473-3099(18)30718-7 · Cited by 134 · see citing works
Fetal Neuropathology in Zika Virus-Infected Pregnant Female Rhesus Monkeys
Martinot, A. J., Abbink, P., Afacan, O., Prohl, A. K., Bronson, R. T., Hecht, J. L., Borducchi, E. N., Larocca, R. A., Peterson, R., Rinaldi, W. J., et al. (2018) · Cell · doi:10.1016/j.cell.2018.03.019 · Cited by 121 · see citing works
Ansarah-Sobrinho, C., Nelson, S., Jost, C. A., Whitehead, S. S., & Pierson, T. C. (2008) · Virology · doi:10.1016/j.virol.2008.08.021 · Cited by 113 · see citing works
Self-citationMiscarriage and stillbirth following maternal Zika virus infection in nonhuman primates
Dudley, D. M., Rompay, K. K. A. V., Coffey, L. L., et al. (2018) · Nature Medicine · doi:10.1038/s41591-018-0088-5 · Cited by 108 · see citing works
Antigen‐specific cytotoxic T lymphocytes protect against lethal West Nile virus encephalitis
Purtha, W. E., Myers, N. B., Mitaksov, V., Sitati, E. M., Connolly, J. M., Fremont, D. H., Hansen, T. H., & Diamond, M. (2007) · European Journal of Immunology · doi:10.1002/eji.200737192 · Cited by 104 · see citing works
Self-citationCross-reactive Dengue virus-specific CD8+ T cells protect against Zika virus during pregnancy
Regla-Nava, J. Á., Ngono, A. E., Viramontes, K. M., Huynh, A.-T., Wang, Y.-T., Nguyen, A.-V. T., Salgado, R., Mamidi, A., Kim, K., Diamond, M., et al. (2018) · Nature Communications · doi:10.1038/s41467-018-05458-0 · Cited by 98 · see citing works
Self-citationQuantitation of rare memory B cell populations by two independent and complementary approaches
Amanna, I. J., & Slifka, M. K. (2006) · Journal of Immunological Methods · doi:10.1016/j.jim.2006.09.005 · Cited by 92 · see citing works
Zika vaccines and therapeutics: landscape analysis and challenges ahead
Wilder‐Smith, A., Vannice, K., Durbin, A. P., Hombach, J., Thomas, S. J., Thevarjan, I., & Simmons, C. P. (2018) · BMC Medicine · doi:10.1186/s12916-018-1067-x · Cited by 90 · see citing works
Dowd, K. A., DeMaso, C. R., & Pierson, T. C. (2015) · mBio · doi:10.1128/mbio.01559-15 · Cited by 81 · see citing works
Self-citationWilliams, K. L., Sukupolvi-Petty, S., Beltramello, M., et al. (2013) · PLoS Pathogens · doi:10.1371/journal.ppat.1003157 · Cited by 79 · see citing works
Animal Models of Zika Virus Infection during Pregnancy
Caine, E. A., Jagger, B. W., & Diamond, M. (2018) · Viruses · doi:10.3390/v10110598 · Cited by 72 · see citing works
Self-citationZika virus vaccines: immune response, current status, and future challenges
Richner, J. M., & Diamond, M. (2018) · Current Opinion in Immunology · doi:10.1016/j.coi.2018.04.024 · Cited by 59 · see citing works
Self-citationXie, X., Kum, D. B., Xia, H., et al. (2018) · Cell Host & Microbe · doi:10.1016/j.chom.2018.09.008 · Cited by 52 · see citing works
Stanelle‐Bertram, S., Gnirß, K., Speiseder, T., Thiele, S., Asante, I. A., Dreier, C., Kouassi, N. M., Preuß, A., Pilnitz-Stolze, G., Müller, U., et al. (2018) · Nature Microbiology · doi:10.1038/s41564-018-0236-1 · Cited by 29 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
mRNA vaccines for infectious diseases: principles, delivery and clinical translation
Chaudhary, N., Weissman, D., & Whitehead, K. A. (2021) · Nature Reviews Drug Discovery · doi:10.1038/s41573-021-00283-5 · Cited by 1,555 · see citing works
Advances in COVID-19 mRNA vaccine development
Fang, E., Liu, X., Li, M., Zhang, Z., Song, L., Zhu, B., Wu, X., Liu, J., Zhao, D., & Li, Y. (2022) · Signal Transduction and Targeted Therapy · doi:10.1038/s41392-022-00950-y · Cited by 601 · see citing works
Recent advances in mRNA vaccine technology
Pardi, N., Hogan, M. J., & Weissman, D. (2020) · Current Opinion in Immunology · doi:10.1016/j.coi.2020.01.008 · Cited by 512 · see citing works
The promise of mRNA vaccines: a biotech and industrial perspective
Jackson, N. A. C., Kester, K. E., Casimiro, D., Gurunathan, S., & DeRosa, F. (2020) · npj Vaccines · doi:10.1038/s41541-020-0159-8 · Cited by 498 · see citing works
A Comprehensive Review of mRNA Vaccines
Gote, V., Bolla, P. K., Kommineni, N., Butreddy, A., Nukala, P. K., Palakurthi, S. S., & Khan, W. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms24032700 · Cited by 457 · see citing works
Pandey, M., Ojha, D., Bansal, S., Rode, A. B., & Chawla, G. (2021) · Molecular Aspects of Medicine · doi:10.1016/j.mam.2021.101003 · Cited by 23 · see citing works
- Authors
- Jagger, B. W., Dowd, K. A., Chen, R. E., Desai, P., Foreman, B., Burgomaster, K. E., Himansu, S., Kong, W.-P., Graham, B. S., Pierson, T. C., & Diamond, M. S.
- Affiliations
- Washington University in St. Louis (US)
- National Institutes of Health (US)
- Moderna Therapeutics (United States) (US)
- Corresponding author
- Jagger, Brett W
- Diamond, Michael S
- ORCID
- Venue
- The Journal of Infectious Diseases
- Publisher
- Oxford University Press (OUP)
- Published
- 2019-07-01
- Volume
- 220
- Issue
- 10
- Pages
- 1577-1588
- ISSN
- 0022-1899 (ISSN-L)
- 1537-6613
- PMC
- PMC6782106
- OpenAlex
- W2979623595
- Cited by
- 53
- References
- 49
- Citations by year
- 2026: 3 · 2025: 5 · 2024: 6 · 2023: 11 · 2022: 6 · 2021: 10 · 2020: 11 · 2019: 1
- FWCI
- 6.45
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model (vor)
- Locations
- The Journal of Infectious Diseases (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (R01 AI073755, R01 HD091218, T32 AI007172)
- National Institute of Allergy and Infectious Diseases Intramural Research
- Moderna
- Topics
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Mosquito-borne diseases and control
- Health Sciences › Medicine › Infectious Diseases › SARS-CoV-2 and COVID-19 Research
- Health Sciences › Medicine › Infectious Diseases › Viral Infections and Outbreaks Research
- Keywords
- Zika virus, nucleic acid vaccines, mRNA vaccines, DNA plasmid vaccine, congenital Zika infection, vertical transmission, pregnancy, antibody-dependent enhancement, long-lived plasma cells, memory B cells, neutralizing antibodies, type I interferon signaling, vaccine efficacy
- MeSH terms
- Zika Virus Infection, Zika Virus, Animals, Antibodies, Viral, B-Lymphocytes, Female, Mice, Inbred C57BL, Mice, Transgenic, Plasma Cells, Pregnancy, Viral Vaccines, Infectious Disease Transmission, Vertical, Vaccines, DNA, Antibodies, Neutralizing
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
