Rhodamine 6G efflux for the detection of CDR1-overexpressing azole-resistant Candidaalbicans strains
Open accessAbstract
We investigated the drug efflux mechanism in azole-resistant strains of Candida albicans using rhodamine 6G (R6G). No significant differences in R6G uptake were observed between azole-sensitive B2630 (9.02 +/- 0.02 nmol/10(8) cells) and azole-resistant B67081 (8.86 +/- 0.03 nmol/10(8) cells) strains incubated in glucose-free phosphate buffered saline. A significantly higher R6G efflux (2.0 +/- 0.21 nmol/10(8) cells) was noted in the azole-resistant strain (B67081) when glucose was added, compared with that in the sensitive strain B2630 (0.23 < or = 0.14 nmol/10(8) cells). A fluconazole-resistant strain C40 that expressed the benomyl resistance gene (CaMDR) also showed a low R6G efflux (0.16 +/- 0.06 nmol/10(8) cells) as did the sensitive strains. Accumulation of R6G in growing C. albicans cells was inversely correlated with the level of CDR1 mRNA expression. Our data also suggest that measurement of intracellular accumulation of R6G is a useful method for identification of azole-resistant strains due to CDR1-expressed drug efflux pump.
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Rhodamine 6G displays substrate specificity to the ABC membrane transporters.
“Rhodamine 6G has been shown to display a substrate specificity to the ABC membrane transporters54.”
From Repurposing approach identifies pitavastatin as a potent azole chemosensitizing agent effective against azole-resistant Candida species (2020) · cites this paper as [54]
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- Authors
- Maesaki, S., Marichal, P., Bossche, H. V., Sanglard, D., & Kohno, S.
- Affiliations
- Nagasaki University (JP)
- ORCID
- Sanglard, Dominique
- Venue
- Journal of Antimicrobial Chemotherapy
- Publisher
- Oxford University Press (OUP)
- Published
- 1999-07
- Volume
- 44
- Issue
- 1
- Pages
- 27-31
- ISSN
- 0305-7453 (ISSN-L)
- 1460-2091
- OpenAlex
- W1975053083
- Cited by
- 162
- References
- 13
- Citations by year
- 2026: 10 · 2025: 8 · 2024: 10 · 2023: 9 · 2022: 7 · 2021: 8 · 2020: 10 · 2019: 8 · 2018: 6 · 2017: 10 · 2016: 7 · 2015: 3 · 2014: 6 · 2013: 8 · 2012: 11
- FWCI
- 1.95
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Journal of Antimicrobial Chemotherapy (publishedVersion)
- PubMed (publishedVersion)
- SERVAL (Université de Lausanne) (publishedVersion, other-oa)
- reroDoc Digital Library (submittedVersion)
- IRIS (submittedVersion)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Health Sciences › Medicine › Oncology › Drug Transport and Resistance Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Keywords
- Candida albicans, azole resistance, CDR1, drug efflux pumps, rhodamine 6G, fluconazole resistance, benomyl resistance
- MeSH terms
- Antifungal Agents, Azoles, Benomyl, Candida albicans, Drug Resistance, Microbial, Coloring Agents, Fungal Proteins, Humans, Microbial Sensitivity Tests, Rhodamines, Genes, MDR, Membrane Transport Proteins
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