Effective approaches to diagnostic stewardship of syndromic molecular panels
Open accessAbstract
Background: Syndromic molecular panels for the diagnosis of gastroenteritis, meningitis/encephalitis, and pneumonia are becoming routinely used for patient care throughout the world. Content: These rapid, sample-to-answer assays have great potential to improve patient care, infection control, and antimicrobial stewardship. However, diagnostic stewardship is essential for their optimal use and accuracy, and interventions can be applied at all phases of the diagnostic process. Summary: The aim of this review article is to describe effective approaches to diagnostic stewardship for syndromic molecular panels to ensure appropriate test utilization and quality assured results.
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Multiplex PCR panels allow simultaneous detection of multiple pathogens directly from cerebrospinal fluid samples, offering rapid results with high analytical sensitivity and specificity.
“These panels allow simultaneous detection of multiple pathogens directly from cerebrospinal fluid (CSF) samples, offering rapid results with high analytical sensitivity and specificity.2”
From Utility and clinical relevance of multiplex PCR panels for diagnosis of central nervous system infections (2026) · cites this paper as [2]
The Meningitis/Encephalitis Panel section says the FilmArray panel detects 14 pathogens from CSF in under an hour and reports mean sensitivity of 92.1% and specificity of 99.2%. Those figures support the claim of rapid detection with high sensitivity and specificity, but the passage reports diagnostic test accuracy, not specifically analytical sensitivity and specificity. No page number is provided in our copy. (LLM, conf=0.82)
Experimental — accuracy not yet measuredEvidence on FAME is still confined to observational, usually retrospective, and single-center studies.
“Evidence is still confined to observational, usually retrospective, and single-center studies (9).”
From Optimized use of the FilmArray Meningitis/Encephalitis panel for early discontinuation of antibiotic therapy (2026) · cites this paper as [9]
The Meningitis/Encephalitis Panel section discusses FAMEP findings and cites a meta-analysis, but does not characterize the underlying studies as observational, retrospective, or single-center. Searches for those study-design terms and for limitations of FAMEP evidence found no passage establishing the claim. (LLM, conf=0.72)
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Cites
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Nicolau-Guillaumet, N., Moutel, M., Plouzeau-Jayle, C., Pean de Ponfilly, G., Courbin, V., Ranc, A.-G., Revillet, H., Mourvillier, B., Hentzien, M., Muggeo, A., et al. (2026) · Microbiology Spectrum · doi:10.1128/spectrum.00566-26 · Cited by 1 · see citing works
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“2 Compared to conventional methods, they provide several advantages: faster turnaround time, broader coverage, and reliable performance even in patients who have already received empirical antimicrobial treatment, which often reduces culture sensitivity.”
- Authors
- Hitchcock, M. M., Gomez, C. A., Pozdol, J., & Banaei, N.
- Affiliations
- Virginia Commonwealth University (US)
- Department of Medicine, Division of Infectious Diseases, University of Nebraska Medical Center , Omaha, NE , United States
- Stanford Medicine (US)
- Corresponding author
- Banaei, Niaz
- Venue
- The Journal of Applied Laboratory Medicine
- Publisher
- Oxford University Press (OUP)
- Published
- 2024-01
- Volume
- 9
- Issue
- 1
- Pages
- 104-115
- ISSN
- 2475-7241 (ISSN-L)
- OpenAlex
- W4390547457
- Cited by
- 20
- References
- 44
- Citations by year
- 2026: 8 · 2025: 6 · 2024: 6
- FWCI
- 1.14
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- The Journal of Applied Laboratory Medicine (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute of Allergy and Infectious Diseases (T32AI052073, T32AI007502)
- Topics
- Health Sciences › Medicine › Physiology › Clinical Laboratory Practices and Quality Control
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Clinical Biochemistry › Bacterial Identification and Susceptibility Testing
- Life Sciences › Immunology and Microbiology › Applied Microbiology and Biotechnology › Antibiotic Use and Resistance
- Keywords
- diagnostic stewardship, rapid molecular diagnostics, antimicrobial stewardship, infection control, test utilization, diagnostic accuracy, gastroenteritis, pneumonia
- MeSH terms
- Antimicrobial Stewardship, Gastroenteritis, Humans, Molecular Diagnostic Techniques
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