Utilization, yield, and accuracy of the filmarray meningitis/encephalitis panel with diagnostic stewardship and testing algorithm
Open accessAbstract
The impact of diagnostic stewardship and testing algorithms on the utilization and performance of the FilmArray meningitis/encephalitis (ME) panel has received limited investigation. We performed a retrospective single-center cohort study assessing all individuals with suspected ME between February 2017 and April 2019 for whom the ME panel was ordered. Testing was restricted to patients with cerebrospinal fluid (CSF) pleocytosis. Positive ME panel results were confirmed before reporting through correlation with direct staining (Gram and calcofluor white) and CSF cryptococcal antigen or by repeat ME panel testing.
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Numerous laboratories encounter difficulty in defining rational restriction criteria in the absence of sufficient real-world data.
“As a result, numerous laboratories encounter difficulty in defining rational restriction criteria in the absence of sufficient real‐world data [25].”
From Real‐World Evaluation of the BioFire FilmArray ME Panel: Diagnostic Accuracy and Clinical Utility (2026) · cites this paper as [25]
The use of syndromic testing through the ME panel confers several meaningful advantages, including markedly faster diagnostic reporting, earlier initiation of targeted therapy, more timely discontinuation of unnecessary antimicrobial agents, and a reduced need for further investigative testing.
“The use of syndromic testing through the ME panel confers several meaningful advantages, including markedly faster diagnostic reporting, earlier initiation of targeted therapy, more timely discontinuation of unnecessary antimicrobial agents, and a reduced need for further investigative testing [25].”
From Real‐World Evaluation of the BioFire FilmArray ME Panel: Diagnostic Accuracy and Clinical Utility (2026) · cites this paper as [25]
Implementation of the ME Panel has been shown to reduce acyclovir days of therapy in several clinical pre-post analyses.
“Implementation of the ME Panel has also been shown to reduce acyclovir days of therapy in several clinical pre-post analyses [12,13,14,15].”
The reduction in acyclovir days of therapy mirrors findings from other studies.
“This mirrors findings from other studies, as previously discussed [12,13,14,15].”
Diagnostic stewardship and development of a diagnostic decision rule with a stepwise approach may improve appropriate use of the ME panel.
“Several studies showed that diagnostic stewardship and the development of a diagnostic decision rule with a stepwise approach may improve the appropriate use of the ME Panel (12–14).”
From Utilization of a Meningitis/Encephalitis PCR panel at the University Hospital Basel – a retrospective study to develop a diagnostic decision rule (2024) · cites this paper as [14]
The ME panel positivity rate at the University Hospital Basel was similar to previously published rates.
“The ME panel positivity rate at our institution was similar as previously published.”
From Utilization of a Meningitis/Encephalitis PCR panel at the University Hospital Basel – a retrospective study to develop a diagnostic decision rule (2024) · cites this paper as [14]
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- Authors
- Broadhurst, M. J., Dujari, S., Budvytiene, I., Pinsky, B. A., Gold, C. A., & Banaei, N.
- Affiliations
- Stanford Medicine (US)
- Venue
- Journal of Clinical Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2020-08-24
- Volume
- 58
- Issue
- 9
- ISSN
- 0095-1137 (ISSN-L)
- 1098-660X
- PMC
- PMC7448656
- OpenAlex
- W3030897705
- Cited by
- 57
- References
- 24
- Citations by year
- 2026: 11 · 2025: 6 · 2024: 11 · 2023: 8 · 2022: 12 · 2021: 7 · 2020: 2
- FWCI
- 4.80
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Journal of Clinical Microbiology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Clinical Biochemistry › Bacterial Identification and Susceptibility Testing
- Life Sciences › Immunology and Microbiology › Microbiology › Bacterial Infections and Vaccines
- Health Sciences › Medicine › Infectious Diseases › Infectious Encephalopathies and Encephalitis
- Keywords
- FilmArray Meningitis/Encephalitis panel, diagnostic stewardship, testing algorithms, cerebrospinal fluid pleocytosis, meningitis, encephalitis, molecular diagnosis, cryptococcal antigen, Gram staining, calcofluor white, diagnostic yield, diagnostic accuracy
- MeSH terms
- Algorithms, Encephalitis, Humans, Meningitis, Retrospective Studies, Cohort Studies
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