Methanogenic potential of tropical feeds rich in hydrolyzable tannins1,2
Open accessAbstract
The present study was carried out to determine the effect of Acacia nilotica, a tropical plant rich in hydrolyzable tannins (HT), on rumen fermentation and methane (CH4) production in vitro. We used leaves and pods from A. nilotica alone and combined. The combination of HT from A. nilotica leaves and pods and condensed tannins (CT) from Calliandra calothyrsus and Leucaena leucocephala were also evaluated to assess potential differences in biological activity between HT and CT. Four series of 24-h incubations were performed using rumen contents of 4 sheep fed a tropical grass (natural grassland based on Dichanthium spp.). A first experiment tested different levels of replacement of this tropical forage (control [CTL] without tannins) by A. nilotica leaves or pods: 0:100, 25:75, 50:50, 75:25 and 100:0. A second experiment tested the mixture of A. nilotica leaves and pods in different proportions: 100:0, 75:25, 50:50, 25:75, and 0:100. A third experiment tested the 50:50 combination of A. nilotica leaves or pods with C. calothyrsus and L. leucocephala. Acacia nilotica pods and leaves had a high content of HT (350 and 178 g/kg DM, respectively), whereas C. calothyrsus and L. leucocephala had a high content of CT (361 and 180 g/kg DM, respectively). The inclusion of HT from A. nilotica leaves and pods decreased CH4 production dose-dependently (P < 0.01). Total replacement of the CTL by A. nilotica decreased CH4 production by 64 and 55% with leaves and pods, respectively. Pods were richer in HT than leaves, but their antimethanogenic effect did not differ (P > 0.05). Although A. nilotica leaves and pods inhibited fermentation, as indicated by the lower gas production and VFA production (P < 0.01), this effect was less pronounced than for CH4. Volatile fatty acid production decreased by 12% in leaves and by 30% in pods when compared with the CTL alone. Positive associative effect was reported for VFA, when HT-rich sources and CT-rich sources were mixed. Combining the 2 sources of HT did not show associative effects on fermentation or CH4 production (P > 0.05). Hydrolyzable tannin-rich sources were more effective in suppressing methanogenesis than CT-rich sources. Our results show that HT-rich A. nilotica leaves and pods have the potential to reduce ruminal CH4 production.
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In vitro hydrolyzable tannin from A. nilotica is more promising for suppressing methanogenesis than condensed tannin from C. calothyrsus and L. leucocephala.
“Contrarily, Rira et al. (196) reported that in vitro HT (A. nilotica) are more promising for suppressing methanogenesis than CT (from C. calothyrsus and L. leucocephala).”
From Phytogenic Additives Can Modulate Rumen Microbiome to Mediate Fermentation Kinetics and Methanogenesis Through Exploiting Diet–Microbe Interaction (2020) · cites this paper as [196]
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- Authors
- Rira, M., Morgavi, D. P., Genestoux, L., Djibiri, S., Sekhri, I., & Doreau, M.
- Affiliations
- VetAgro Sup (FR)
- Ecole Nationale Supérieure de Biotechnologie Taoufik Khaznadar (DZ)
- Corresponding author
- Rira, Moufida
- ORCID
- Morgavi, Diego P
- Venue
- Journal of Animal Science
- Publisher
- Oxford University Press (OUP)
- Published
- 2019-06-13
- Volume
- 97
- Issue
- 7
- Pages
- 2700-2710
- ISSN
- 0021-8812 (ISSN-L)
- 1525-3163
- PMC
- PMC6606517
- OpenAlex
- W2952839616
- Cited by
- 28
- References
- 49
- Citations by year
- 2025: 4 · 2024: 2 · 2023: 2 · 2022: 3 · 2021: 9 · 2020: 5 · 2019: 3
- FWCI
- 7.02
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model (vor)
- Locations
- Journal of Animal Science (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion)
- Funders
- AnimalChange
- European Community's Seventh Framework Program
- Topics
- Life Sciences › Agricultural and Biological Sciences › Agronomy and Crop Science › Ruminant Nutrition and Digestive Physiology
- Physical Sciences › Engineering › Building and Construction › Anaerobic Digestion and Biogas Production
- Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Nutrition and Physiology
- Keywords
- methanogenesis, hydrolysable tannins, proanthocyanidins, Acacia nilotica, Calliandra calothyrsus, Leucaena leucocephala, rumen fermentation, methane mitigation, volatile fatty acids, tropical forages, sheep, in vitro rumen incubation, Dichanthium
- MeSH terms
- Acacia, Animals, Animal Feed, Diet, Fatty Acids, Volatile, Fermentation, Fruit, Poaceae, Hydrolysis, Fabaceae, Methane, Rumen, Sheep, Plant Leaves, Proanthocyanidins, Hydrolyzable Tannins
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