Difference in the nature of tannins on in vitro ruminal methane and volatile fatty acid production and on methanogenic archaea and protozoal populations
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Six plant sources of hydrolyzable tannins (HT) or HT and condensed tannins (CT; designated as HT1, HT2, HT3, HT + CT1, HT + CT2, and HT + CT3) were evaluated to determine their effects in vitro on CH(4) production and on ruminal archaeal and protozoa populations, and to assess potential differences in biological activities between sources containing HT only or HT and CT. Samples HT1, HT2, and HT3 contained only HT, whereas samples HT + CT1, HT + CT2, and HT + CT3 contained HT and CT. In experiment 1, in vitro incubations with samples containing HT or HT + CT resulted in a decrease in CH(4) production of 0.6 and 5.5%, respectively, compared with that produced by incubations containing the added tannin binder polyethylene glycol-6000. Tannin also suppressed the population of methanogenic archaea in all incubations except those with HT2, with an average decrease of 11.6% in HT incubations (15.8, 7.09, and 12.0 in HT1, HT2, and HT3) and 28.6% in incubations containing HT + CT (35.0, 40.1, and 10.8 in HT + CT1, HT + CT2, and HT + CT3) when compared with incubations containing added polyethylene glycol-6000. The mean decrease in protozoal counts was 12.3% in HT and 36.2% in HT + CT incubations. Tannins increased in vitro pH, reduced total VFA concentrations, increased propionate concentrations, and decreased concentrations of iso-acids. In experiment 2, when a basal diet was incubated with graded levels of HT + CT1, HT + CT2, and HT + CT3, the total gas and CH4 production and archaeal and protozoal populations decreased as the concentration of tannins increased. Our results confirm that tannins suppress methanogenesis by reducing methanogenic populations in the rumen either directly or by reducing the protozoal population, thereby reducing methanogens symbiotically associated with the protozoal population. In addition, tannin sources containing both HT and CT were more potent in suppressing methanogenesis than those containing only HT.
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Addition of condensed tannin extracts to the diet reduces populations of methanogenic archaea and some cellulolytic bacteria (R. flavefaciens).
“The addition of CT extracts to the diet reduced populations of methanogenic archaea and some cellulolytic bacteria (R. flavefaciens) (201).”
From Phytogenic Additives Can Modulate Rumen Microbiome to Mediate Fermentation Kinetics and Methanogenesis Through Exploiting Diet–Microbe Interaction (2020) · cites this paper as [201]
The Discussion reports that incubating a basal diet with tannin sources containing both hydrolyzable and condensed tannins reduced methanogenic archaeal populations. It does not report a reduction in R. flavefaciens; the study measured total bacterial counts, which increased in one set of HT + CT incubations. Its findings are from in vitro incubation, not animals fed a diet. (LLM, conf=0.88)
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- Authors
- Bhatta, R., Uyeno, Y., Tajima, K., Takenaka, A., Yabumoto, Y., Nonaka, I., Enishi, O., & Kurihara, M.
- Affiliations
- Institute of Livestock and Grassland Science (JP)
- National Institute of Advanced Industrial Science and Technology (JP)
- Corresponding author
- Bhatta, R.
- Venue
- Journal of Dairy Science
- Publisher
- American Dairy Science Association
- Published
- 2009-11
- Volume
- 92
- Issue
- 11
- Pages
- 5512-5522
- ISSN
- 0022-0302 (ISSN-L)
- OpenAlex
- W2135206137
- Cited by
- 359
- References
- 40
- Citations by year
- 2026: 20 · 2025: 22 · 2024: 25 · 2023: 29 · 2022: 24 · 2021: 33 · 2020: 24 · 2019: 31 · 2018: 24 · 2017: 18 · 2016: 24 · 2015: 23 · 2014: 14 · 2013: 11 · 2012: 16
- FWCI
- 26.11
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Journal of Dairy Science (publishedVersion)
- PubMed (publishedVersion)
- Funders
- U.S. Department of Agriculture
- Ministry of Environment
- Ministry of Agriculture, Forestry and Fisheries
- Japan Society for the Promotion of Science
- Topics
- Life Sciences › Agricultural and Biological Sciences › Agronomy and Crop Science › Ruminant Nutrition and Digestive Physiology
- Physical Sciences › Engineering › Building and Construction › Anaerobic Digestion and Biogas Production
- Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Nutrition and Physiology
- Keywords
- tannins, hydrolysable tannins, proanthocyanidins, ruminal methane, methanogenesis, methanogenic archaea, rumen protozoa, volatile fatty acids, propionate, methane mitigation, polyethylene glycol 6000, in vitro rumen fermentation, rumen pH
- MeSH terms
- Animals, Archaea, Cattle, Fatty Acids, Volatile, Female, Methane, Polyethylene Glycols, RNA, Ribosomal, 16S, Rumen, Surface-Active Agents, Tannins, Ciliophora
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