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Cited by 24Bone Metabolism and DiseasesCellular transport and secretionBone and Dental Protein Studies

The ruffled border and attachment regions of the apposing membrane of resorbing osteoclasts as visualized from the cytoplasmic face of the membrane

Akisaka, T.·2006·Journal of Electron Microscopy
DOI 10.1093/jmicro/dfl012
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Abstract

The aim of our present research was to visualize how the plasma membrane is modified and how the cytoskeleton interacts with the attachment and ruffled border regions of resorbing osteoclasts. In order to view the surface modification of membranes and associated cytoskeleton, we employed the method of cell-shearing combined with quick-freezing and rotary replication to expose and replicate an extensive area of the cytoplasmic face of the surface membrane of osteoclasts in contact with synthetic apatite as a substratum. The membrane apposed to the apatite was composed of three different domains: the attachment zone, ruffled border and the remainder. In the attachment zone, a highly organized actin filament network formed dot-shaped, F-actin rich adhesion sites, so-called podosomes, and the actin ring. The cytoskeletal filament of podosomes and actin ring appeared to be in direct contact with the cytoplasmic surface of the underlying membrane. Within the actin ring, individually recognizable podosomes were well preserved, which indicates that the actin ring was probably derived from the fusion of podosomes. After shearing at the ruffled border region, the ruffled border projections and membrane regions among the projections were left behind. These ruffled border projections contained the cytoskeletal network. These actin networks also appeared to be in direct contact with the inner side of the ruffled border membrane or in contact with it via membrane-associated particles. At the basal portion of the ruffled border, numerous clathrin-coated patches or pits were well preserved. Deeper clathrin-coated pits and vesicles were also found, which indicates an active site for receptor-mediated endocytotic events. Clathrin sheets were also observed in the cell periphery outside of the actin ring. This type of clathrin sheets adhered to the apatite substrate, but was not anchored to the actin microfilaments. Our study thus clearly visualized the interaction between the cytoskeletal filaments and the underlying membrane at the ruffled border, attachment zone and podosome in osteoclasts cultured on apatitepellets.

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Crossref · 10.1093/jmicro/dfl012

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  • Mature osteoclasts are polarized cells with an apical membrane facing bone and an opposite basolateral membrane facing plasma; both domains are essential for resorbing osteoclasts.

    “Mature osteoclasts are polarized cells with an apical membrane facing bone and an opposite basolateral membrane facing plasma. Both apical and basolateral membrane domains are essential structures for resorbing osteoclasts.185”

    From Bone remodeling: an operational process ensuring survival and bone mechanical competence (2022) · cites this paper as [185]

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Authors
Akisaka, T.
Affiliations
Asahi University (JP)
Corresponding author
Akisaka, T.
Venue
Journal of Electron Microscopy
Publisher
Oxford University Press (OUP)
Published
2006-05-15
Volume
55
Issue
2
Pages
53-61
ISSN
  • 0022-0744 (ISSN-L)
  • 1477-9986
DOI
10.1093/jmicro/dfl012
OpenAlex
W2150894337
Cited by
24
References
37
Citations by year
2025: 1 · 2022: 1 · 2021: 1 · 2020: 1 · 2019: 2 · 2018: 2 · 2016: 1 · 2015: 1 · 2014: 1 · 2013: 1 · 2012: 1
FWCI
1.22
Type
journal article
Language
en
Open access
closed
Locations
  • Journal of Electron Microscopy (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bone Metabolism and Diseases
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
  • Health Sciences › Medicine › Rheumatology › Bone and Dental Protein Studies
Keywords
osteoclasts, ruffled border, plasma membrane, podosomes, actin rings, actin cytoskeleton, clathrin-coated pits, receptor-mediated endocytosis, membrane surface modification
MeSH terms
Animals, Bone Resorption, Cell Fractionation, Cell Membrane, Cells, Cultured, Coated Pits, Cell-Membrane, Cytological Techniques, Cytoskeleton, Microscopy, Electron, Microscopy, Electron, Scanning, Microscopy, Fluorescence, Osteoclasts, Rabbits, Clathrin-Coated Vesicles

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