Cited by 60Muscle metabolism and nutritionFood and Agricultural SciencesFood composition and properties

[15N]-labeled pea flour protein nitrogen exhibits good ileal digestibility and postprandial retention in humans

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Gausserès, Nicolas, Mahé, Sylvain, Benamouzig, Robert, Luengo, Catherine, Ferriere, Francoise, Rautureau, Jacques, Tomé, Daniel·1997·The Journal of Nutrition

Abstract

The aim of the present study was to evaluate postprandial absorption of pea protein as well as exogenous nitrogen retention in humans. For this purpose, after fasting overnight, seven healthy adults (4 males and 3 females) ingested [15N]-labeled pea protein (195 mmol N). Ileal effluents were collected for 8 h at 30-min intervals using a nasointestinal intubation technique. Urine and plasma samples were collected for 24 h. The [15N]-enrichment was determined in the intestinal samples, in the plasma amino acids and urea as well as in the urinary urea and ammonia fractions. The true gastroileal absorption of pea protein was 89.4 +/- 1.1%. This absorption was correlated with a significant increase (P < 0.05) in [15N]-enrichment in the plasma amino acids and in the nitrogen incorporated into the body urea pool for 1 h following pea ingestion. The enrichment remained significantly higher than the basal values in these pools 24 h after pea ingestion. The recovery of total urinary exogenous nitrogen after 22 h was 31.1 +/- 9.3 mmol N. Moreover, the kinetics of [15N]-labeled pea amino acids deamination reached a plateau of 39 mmol. Under these conditions, pea nitrogen retention represented 78% of the absorbed dietary nitrogen in healthy humans. The present results demonstrate the good true nitrogen digestibility and retention of pea protein in humans.

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Crossref · 10.1093/jn/127.6.1160

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  • Differences in protein absorbability are ameliorated after protein purification through removal of nonprotein constituents to produce protein isolates.

    “However, differences in protein absorbability are ameliorated after protein purification through removal of nonprotein constituents to produce protein isolates [[80], [81], [82]].”

    From Nutritional Considerations for the Vegan Athlete (2023) · cites this paper as [81]

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Authors
Gausserès, N., Mahé, S., Benamouzig, R., Luengo, C., Ferriere, F., Rautureau, J., & Tomé, D.
Affiliations
  • Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement (FR)
  • Hôpital Avicenne (FR)
Corresponding author
Mahé, Sylvain
Venue
The Journal of Nutrition
Publisher
Elsevier BV
Published
1997-06
Volume
127
Issue
6
Pages
1160-1165
ISSN
0022-3166 (ISSN-L)
OpenAlex
W2158558972
Cited by
60
References
38
Citations by year
2026: 1 · 2025: 3 · 2024: 5 · 2023: 3 · 2021: 4 · 2020: 3 · 2019: 4 · 2018: 2 · 2017: 1 · 2016: 1 · 2013: 2 · 2012: 1
FWCI
0.99
Type
journal article
Language
en
Open access
Locations
  • Journal of Nutrition (publishedVersion)
  • PubMed (publishedVersion)
  • HAL (submittedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Muscle metabolism and nutrition
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Food and Agricultural Sciences
  • Health Sciences › Nursing › Nutrition and Dietetics › Food composition and properties
Keywords
pea protein, nitrogen digestibility, nitrogen retention, ileal digestibility, urinary nitrogen excretion, amino acid deamination, nitrogen balance, legume protein
MeSH terms
Adult, Digestion, Female, Humans, Ileum, Intestinal Absorption, Male, Nitrogen, Plant Proteins, Dietary, Pisum sativum, Postprandial Period

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