Conservation of sequence in recombination signal sequence spacers

Ramsden, Dale A.; Baetz, Kristin; Wu, Gillian E.

doi:10.1093/nar/22.10.1785

Cited by 175DNA Repair MechanismsGenomics and Chromatin DynamicsT-cell and B-cell Immunology

Conservation of sequence in recombination signal sequence spacers

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Ramsden, Dale A., Baetz, Kristin, Wu, Gillian E.·1994·Nucleic Acids Research

DOI 10.1093/nar/22.10.1785PMC PMC308075

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Abstract

The variable domains of immunoglobulins and T cell receptors are assembled through the somatic, site specific recombination of multiple germline segments (V, D, and J segments) or V(D)J rearrangement. The recombination signal sequence (RSS) is necessary and sufficient for cell type specific targeting of the V(D)J rearrangement machinery to these germline segments. Previously, the RSS has been described as possessing both a conserved heptamer and a conserved nonamer motif. The heptamer and nonamer motifs are separated by a 'spacer' that was not thought to possess significant sequence conservation, however the length of the spacer could be either 12 +/- 1 bp or 23 +/- 1 bp long. In this report we have assembled and analyzed an extensive data base of published RSS. We have derived, through extensive consensus comparison, a more detailed description of the RSS than has previously been reported. Our analysis indicates that RSS spacers possess significant conservation of sequence, and that the conserved sequence in 12 bp spacers is similar to the conserved sequence in the first half of 23 bp spacers.

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The structure of Transib TIRs corresponds closely to the structure of RSSs, which are composed of conserved heptamers 5′-CACAGTG separated by variable 22-bp spacers from ACAAAAACC-like nonamers.
“This corresponds closely to the structure of RSSs, which are composed of the conserved heptamers 5′- CACAGTG separated by a variable 22-bp spacers from ACAAAAACC-like nonamers [1,5–7].”
From RAG1 Core and V(D)J Recombination Signal Sequences Were Derived from Transib Transposons (2005) · cites this paper as [6]

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RAG1 Core and V(D)J Recombination Signal Sequences Were Derived from Transib Transposons
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Authors: Ramsden, D. A., Baetz, K., & Wu, G. E.
Affiliations: University of Toronto (CA)
Corresponding author: Wu, Gillian E.
ORCID: Ramsden, Dale A.
Baetz, Kristin
Venue: Nucleic Acids Research
Publisher: Oxford University Press (OUP)
Published: 1994
Volume: 22
Issue: 10
Pages: 1785-1796
ISSN: 0305-1048 (ISSN-L)
1362-4962
DOI: 10.1093/nar/22.10.1785
PMC: PMC308075
OpenAlex: W2087604899
Cited by: 175
References: 121
Citations by year: 2026: 1 · 2025: 9 · 2024: 4 · 2023: 7 · 2022: 3 · 2021: 5 · 2020: 2 · 2019: 3 · 2018: 6 · 2017: 4 · 2016: 5 · 2015: 4 · 2014: 5 · 2013: 8 · 2012: 1
FWCI: 1.77
Type: journal article
Language: en
Open access: green
europepmc.org
Locations: Nucleic Acids Research (publishedVersion)
Europe PMC (PubMed Central) (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
Funders: Francis Crick Institute
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
Keywords: recombination signal sequences, sequence conservation, V(D)J recombination, immunoglobulin variable region, T-cell receptor variable region
MeSH terms: Animals, Antibody Diversity, Base Composition, Base Sequence, DNA, Genes, Immunoglobulin, Humans, Molecular Sequence Data, Receptors, Antigen, T-Cell, Recombination, Genetic, Gene Rearrangement, Consensus Sequence, Sequence Alignment, Conserved Sequence

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Conservation of sequence in recombination signal sequence spacers

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