A new ATP-independent DNA endonuclease from Schizosaccharomyces pombe that recognizes cyclobutane pyrimidine dimers and 6–4 photoproducts
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We have discovered a new DNA endonuclease in the fission yeast Schizosaccharomyces pombe which recognizes cyclobutane pyrimidine dimers and (6-4) pyrimidine-pyrimidone photoproducts. S. pombe DNA endonuclease (SPDE) catalyzes a single ATP-independent incision immediately 5' to the UV photoproduct and generates termini containing 3' hydroxyl and 5' phosphoryl groups. Based on these properties, we propose that SPDE may function in a DNA repair capacity, representing the initial recognition/cleavage step of a DNA excision repair pathway.
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UVDE initiates repair at both dipyrimidine photoproducts by incision immediately 5′ to the damage, followed by long-patch BER in conjunction with Rad27/Fen1, XRCC1, PARP1, and DNA polymerase.
“UVDE initiates repair at both dipyrimidine photoproducts by incision immediately 5′ to the damage18,28,29, followed by long-patch BER in conjunction with Rad27/Fen1, XRCC1, PARP1, and DNA polymerase30–33.”
From Modulation of UVB-induced Carcinogenesis by Activation of Alternative DNA Repair Pathways (2018) · cites this paper as [28]
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Self-citation
- Authors
- Bowman, K. K., Sidik, K., Smith, C. A., Taylor, J.-S., Doetsch, P. W., & Freyer, G. A.
- Affiliations
- Emory University (US)
- Columbia University (US)
- Washington University in St. Louis (US)
- Corresponding author
- Freyer, Greg A.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 1994
- Volume
- 22
- Issue
- 15
- Pages
- 3026-3032
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- PMC
- PMC310271
- OpenAlex
- W1971759328
- Cited by
- 90
- References
- 24
- Citations by year
- 2026: 1 · 2023: 1 · 2021: 2 · 2020: 1 · 2018: 4 · 2017: 1 · 2015: 1 · 2014: 6 · 2013: 3 · 2012: 2
- FWCI
- 2.02
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Nucleic Acids Research (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- American Cancer Society (CA40463)
- National Institutes of Health (5r01ca055896-04, CA40463)
- National Cancer Institute (CA40463, CA 55896, CA42608)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Keywords
- Schizosaccharomyces pombe, cyclobutane pyrimidine dimers, 6-4 photoproducts, UV photoproducts, excision repair, DNA repair enzymes
- MeSH terms
- Adenosine Triphosphate, Base Sequence, DNA, DNA Damage, DNA Repair, Endodeoxyribonucleases, Molecular Sequence Data, Photochemistry, Pyrimidine Dimers, Schizosaccharomyces, Substrate Specificity, Ultraviolet Rays, Schizosaccharomyces pombe Proteins
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