Immunoprecipitation of Pyrimidine(6-4)pyrimidone Photoproducts and Cyclobutane Pyrimidine Dimers in uv-Irradiated DNA
Abstract
Biological studies suggest that a significant proportion of the cytotoxicity observed in mammalian cells after uv irradiation may be due to damage other than cyclobutane dimers in DNA. Although pyrimidine-pyrimidone (6-4) photoproducts have been implicated as major contributors to cell lethality, their induction has been measured at considerably less than cyclobutane pyrimidine dimers when measured by chromatographic techniques. Because the yield of (6-4) photoproducts may be reduced by their lability to extreme heat and pH, we have advised an alternative, immunological quantification which does not require DNA hydrolysis. Affinity-purified rabbit antisera were used to precipitate low molecular weight 32P-labeled PM2 DNA irradiated with increasing fluences of uv light. DNA of known molecular weight was used to determine rates of induction for antibody-binding sites associated with (6-4) photoproducts and cyclobutane dimers. These rates were calculated to be 0.6 (6-4) photoproducts and 1.2 cyclobutane dimers/10(8) Da/J/m2. At low uv fluences (6-4) photoproducts were induced at one-half the rate of cyclobutane dimers, whereas at higher fluences (6-4) photoproducts predominated.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The ratio between CPD and 6-4PP yields after UVB irradiation is between 3 and 5.
“The values reported range between 3 and 5 (53,55,59).”
From Photoinduced Damage to Cellular DNA: Direct and Photosensitized Reactions<sup>†</sup> (2012) · cites this paper as [59]
Cites
A pyrimidine dimer–DNA glycosylase activity associated with the v gene product of bacteriophage T4
Radany, E. H., & Friedberg, E. C. (1980) · Nature · doi:10.1038/286182a0 · Cited by 165 · see citing works
Thomas, D. C., Okumoto, D. S., Sancar, A., & Bohr, V. A. (1989) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)84671-9 · Cited by 94 · see citing works
Mitchell, D. L., & Rosenstein, B. S. (1987) · Photochemistry and Photobiology · doi:10.1111/j.1751-1097.1987.tb07882.x · Cited by 67 · see citing works
Self-citationMitchell, D. L. (1988) · Mutation Research/DNA Repair Reports · doi:10.1016/0167-8817(88)90024-7 · Cited by 60 · see citing works
Self-citationMitchell, D. L., Humphrey, R. M., Adair, G. M., Thompson, L. H., & Clarkson, J. M. (1988) · Mutation Research/DNA Repair Reports · doi:10.1016/0167-8817(88)90007-7 · Cited by 48 · see citing works
Self-citationRosenstein, B. S., & Setlow, R. B. (1980) · Biophysical Journal · doi:10.1016/s0006-3495(80)85050-8 · Cited by 44 · see citing works
Isolating human DNA repair genes using rodent-cell mutants
Thompson, L. H., Weber, C. A., Brookman, K. W., Salazar, E. P., Stewart, S. A., & Mitchell, D. L. (1987) · University of North Texas Digital Library (University of North Texas) · Cited by 8 · see citing works
Self-citation
Flagged first, then by citations
Cited by
Sorted by citations
Direct and indirect effects of UV radiation on DNA and its components
Ravanat, J.-L., Douki, T., & Cadet, J. (2001) · Journal of Photochemistry and Photobiology B: Biology · doi:10.1016/s1011-1344(01)00206-8 · Cited by 980 · see citing works
Formation of UV-induced DNA damage contributing to skin cancer development
Cadet, J., & Douki, T. (2018) · Photochemical & Photobiological Sciences · doi:10.1039/c7pp00395a · Cited by 435 · see citing works
Photoinduced Damage to Cellular DNA: Direct and Photosensitized Reactions<sup>†</sup>
Cadet, J., Mouret, S., Ravanat, J., & Douki, T. (2012) · Photochemistry and Photobiology · doi:10.1111/j.1751-1097.2012.01200.x · Cited by 300 · see citing works
Douki, T., Court, M., Sauvaigo, S., Odin, F., & Cadet, J. (2000) · Journal of Biological Chemistry · doi:10.1074/jbc.275.16.11678 · Cited by 239 · see citing works
Sage, É. (1993) · Photochemistry and Photobiology · doi:10.1111/j.1751-1097.1993.tb02273.x · Cited by 235 · see citing works
Cadet, J., Anselmino, C., Douki, T., & Voituriez, L. (1992) · Journal of Photochemistry and Photobiology B: Biology · doi:10.1016/1011-1344(92)85135-h · Cited by 211 · see citing works
Lindberg, C., & Horneck, G. (1991) · Journal of Photochemistry and Photobiology B Biology · doi:10.1016/1011-1344(91)80269-n · Cited by 111 · see citing works
UV and ionizing radiations induced DNA damage, differences and similarities
Ravanat, J.-L., & Douki, T. (2016) · Radiation Physics and Chemistry · doi:10.1016/j.radphyschem.2016.07.007 · Cited by 94 · see citing works
XPA protein as a limiting factor for nucleotide excision repair and UV sensitivity in human cells
Köberle, B., Roginskaya, V. Y., & Wood, R. D. (2006) · DNA repair · doi:10.1016/j.dnarep.2005.12.001 · Cited by 91 · see citing works
UV-DDB-dependent regulation of nucleotide excision repair kinetics in living cells
Nishi, R., Alekseev, S., Dinant, C., Hoogstraten, D., Houtsmuller, A. B., Hoeijmakers, J. H. J., Vermeulen, W., Hanaoka, F., & Sugasawa, K. (2009) · DNA Repair · doi:10.1016/j.dnarep.2009.02.004 · Cited by 76 · see citing works
QUANTUM YIELDS AND SECONDARY PHOTOREACTIONS OF THE PHOTOPRODUCTS OF dTpdT, dTpdC AND dTpdU
Lemaire, D. E., & Ruzsicska, B. P. (1993) · Photochemistry and Photobiology · doi:10.1111/j.1751-1097.1993.tb09207.x · Cited by 66 · see citing works
Dany, A.-L., Douki, T., Triantaphylidès, C., & Cadet, J. (2001) · Journal of Photochemistry and Photobiology B Biology · doi:10.1016/s1011-1344(01)00254-8 · Cited by 60 · see citing works
Kan, L. S., Voituriez, L., & Cadet, J. (1992) · Journal of Photochemistry and Photobiology B Biology · doi:10.1016/1011-1344(92)85040-2 · Cited by 39 · see citing works
Douki, T. (2020) · Photochemistry and Photobiology · doi:10.1111/php.13278 · Cited by 32 · see citing works
Assessment of DNA damage and repair in specific genomic regions by quantitative immuno-coupled PCR
Denissenko, M. F., Venkatachalam, S., Yamasaki, E. F., & Wani, A. A. (1994) · Nucleic Acids Research · doi:10.1093/nar/22.12.2351 · Cited by 25 · see citing works
Douki, T., & Cadet, J. (1995) · Birkhäuser Basel eBooks · doi:10.1007/978-3-0348-9061-8_9 · Cited by 20 · see citing works
UVB‐induced formation of (6–4) photoproducts in the rat corneal epithelium
Estil, S., Olsen, W. M., Huitfeldt, H. S., & Haaskjold, E. (1997) · Acta Ophthalmologica Scandinavica · doi:10.1111/j.1600-0420.1997.tb00104.x · Cited by 16 · see citing works
Genome-wide profiles of UV lesion susceptibility, repair, and mutagenic potential in melanoma
Perez, B. S., Wong, K. M., Schwartz, E. K., Herrera, R. E., King, D. A., García-Nieto, P. E., & Morrison, A. J. (2021) · Mutation research. Fundamental and molecular mechanisms of mutagenesis · doi:10.1016/j.mrfmmm.2021.111758 · Cited by 12 · see citing works
UV damage to nucleic acid components
Ravanat, J., Douki, T., & Cadet, J. (2001) · Comprehensive series in photosciences · doi:10.1016/s1568-461x(01)80045-4 · Cited by 9 · see citing works
Huang, Q., Han, X., Tong, Z., Deng, Y., Xie, L., Liu, S., Xie, B., & Zhang, W. (2024) · Journal of Fungi · doi:10.3390/jof10030228 · Cited by 11 · see citing works
Ghosh, R., Johnston, D. A., & Mitchell, D. L. (1995) · Nucleic Acids Research · doi:10.1093/nar/23.23.4934 · Cited by 9 · see citing works
Self-citationVariations in Transcription-Repair Coupling in Mouse Cells
Murad, A. O., de Cock, J., Brown, D. W., & Smerdon, M. J. (1995) · Journal of Biological Chemistry · doi:10.1074/jbc.270.8.3949 · Cited by 9 · see citing works
Latimer, J. J., Majekwana, V. J., Pabón‐Padín, Y. R., Pimpley, M. R., & Grant, S. G. (2014) · Photochemistry and Photobiology · doi:10.1111/php.12387 · Cited by 8 · see citing works
Antibodies and DNA Photoproducts: Applications, Milestones and Reference Guide
Mitchell, D. L., & Brooks, B. (2009) · Photochemistry and Photobiology · doi:10.1111/j.1751-1097.2009.00673.x · Cited by 8 · see citing works
Self-citationIn vitro chromatin templates to study nucleotide excision repair
Liu, X. (2015) · DNA repair · doi:10.1016/j.dnarep.2015.09.026 · Cited by 8 · see citing works
Sunlight-Induced DNA Damage: Molecular Mechanisms and Photoprotection Strategies
Douki, T. (2016) · Springer eBooks · doi:10.1007/978-3-319-43157-4_3 · Cited by 8 · see citing works
The use of suction blisters to measure sunscreen protection against UVR-induced DNA damage
Josse, G., Douki, T., Digabel, J. L., Gravier, E., & Questel, E. (2017) · Journal of Photochemistry and Photobiology B Biology · doi:10.1016/j.jphotobiol.2017.12.021 · Cited by 7 · see citing works
QUANTUM YIELDS AND SECONDARY PHOTOREACTIONS OF THE PHOTOPRODUCTS OF dTpdT, dTpdC AND dTpdU
Lemaire, D. G. E., & Ruzsicska, B. P. (1993) · Photochemistry and Photobiology · doi:10.1111/j.1751-1097.1993.tb09208.x · Cited by 5 · see citing works
Styer, S. C., & Griffiths, T. D. (1992) · Radiation Research · doi:10.2307/3578482 · Cited by 5 · see citing works
Douki, T. (2007) · Royal Society of Chemistry eBooks · doi:10.1039/9781847557957-00225 · Cited by 3 · see citing works
Snapka, R. M. (1996) · Elsevier eBooks · doi:10.1016/b978-012653630-0/50004-0 · Cited by 2 · see citing works
Replication fork stability in mammalian cells
Elvers, I. (2011) · KTH Publication Database DiVA (KTH Royal Institute of Technology)
- Authors
- Mitchell, D. L., Allison, J. P., & Nairn, R. S.
- Affiliations
- The University of Texas MD Anderson Cancer Center (US)
- ORCID
- Allison, James P.
- Venue
- Radiation Research
- Publisher
- JSTOR
- Published
- 1990-09
- Volume
- 123
- Issue
- 3
- Pages
- 299
- ISSN
- 0033-7587 (ISSN-L)
- OpenAlex
- W2057906697
- Cited by
- 32
- References
- 7
- Citations by year
- 2024: 1 · 2021: 1 · 2020: 1 · 2018: 1 · 2017: 1 · 2016: 2 · 2015: 1 · 2014: 1 · 2012: 1
- FWCI
- 1.00
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Radiation Research (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Cancer Institute (CA36361)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Carcinogens and Genotoxicity Assessment
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Effects of Radiation Exposure
- Keywords
- cyclobutane pyrimidine dimers, UV irradiation, DNA damage, immunoprecipitation, antibody binding, cytotoxicity, DNA repair
- MeSH terms
- Animals, DNA, DNA Damage, Precipitin Tests, Pyrimidine Dimers, Rabbits, Ultraviolet Rays
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
