The DOI matches its Crossref record; 51 of the 51 references it cites were found in a registry or an index.
In vivo degradation of RNA polymerase II largest subunit triggered by alpha-amanitin
Open accessAbstract
Alpha-Amanitin is a well-known specific inhibitor of RNA polymerase II (RNAPII) in vitro and in vivo. It is a cyclic octapeptide which binds with high affinity to the largest subunit of RNAPII, RPB1. We have found that in murine fibroblasts exposure to alpha-amanitin triggered degradation of the RPB1 subunit, while other RNAPII subunits, RPB5 and RPB8, remained almost unaffected. Transcriptional inhibition in alpha-amanitin-treated cells was slow and closely followed the disappearance of RPB1. The degradation rate of RPB1 was alpha-amanitin dose dependent and was not a consequence of transcriptional arrest. Alpha-Amanitin-promoted degradation of RPB1 was prevented in cells exposed to actinomycin D, another transcriptional inhibitor. Epitope-tagged recombinant human RPB1 subunits were expressed in mouse fibroblasts. In cells exposed to alpha-amanitin the wild-type recombinant subunit was degraded like the endogenous protein, but a mutated alpha-amanitin-resistant subunit remained unaffected. Hence, alpha-amanitin did not activate a proteolytic system, but instead its binding to mRPB1 likely represented a signal for degradation. Thus, in contrast to other inhibitors, such as actinomycin D or 5,6-dichloro-1-beta-D-ribofuranosyl-benzimidazole, which reversibly act on transcription, inhibition by alpha-amanitin cannot be but an irreversible process because of the destruction of RNAPII.
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Specific Inhibition of Nuclear RNA Polymerase II by α-Amanitin
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Branco, M. R., & Pombo, A. (2006) · PLoS Biology · doi:10.1371/journal.pbio.0040138 · Cited by 694
A Slow RNA Polymerase II Affects Alternative Splicing In Vivo
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Kinetics of Core Histones in Living Human Cells
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In vivo dynamics of RNA polymerase II transcription
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Self-citation
Metadata
- Authors
- Nguyen, V.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 1996-08-01
- Volume
- 24
- Issue
- 15
- Pages
- 2924-2929
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W1990213691
- Cited by
- 265
- References
- 51
- FWCI
- 1.98
- Type
- journal article
- Language
- en
- Open access
- bronze
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