Nucleotide sequence of the iap gene, responsible for alkaline phosphatase isozyme conversion in Escherichia coli, and identification of the gene product
Open accessAbstract
The iap gene in Escherichia coli is responsible for the isozyme conversion of alkaline phosphatase. We analyzed the 1,664-nucleotide sequence of a chromosomal DNA segment that contained the iap gene and its flanking regions. The predicted iap product contained 345 amino acids with an estimated molecular weight of 37,919. The 24-amino-acid sequence at the amino terminus showed features characteristic of a signal peptide. Two proteins of different sizes were identified by the maxicell method, one corresponding to the Iap protein and the other corresponding to the processed product without the signal peptide. Neither the isozyme-converting activity nor labeled Iap proteins were detected in the osmotic-shock fluid of cells carrying a multicopy iap plasmid. The Iap protein seems to be associated with the membrane.
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CRISPR/Cas9 was initially discovered in Escherichia coli.
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From Emerging applications of gene editing technologies for the development of climate-resilient crops (2025) · cites this paper as Ishino et al., 1987
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- Authors
- Ishino, Y., Shinagawa, H., Makino, K., Amemura, M., & Nakata, A.
- Affiliations
- The University of Osaka (JP)
- Venue
- Journal of Bacteriology
- Publisher
- American Society for Microbiology
- Published
- 1987-12
- Volume
- 169
- Issue
- 12
- Pages
- 5429-5433
- ISSN
- 0021-9193 (ISSN-L)
- 1098-5530
- PMC
- PMC213968
- OpenAlex
- W2118746294
- Cited by
- 2,421
- References
- 26
- Citations by year
- 2026: 120 · 2025: 207 · 2024: 233 · 2023: 255 · 2022: 291 · 2021: 272 · 2020: 205 · 2019: 156 · 2018: 156 · 2017: 140 · 2016: 130 · 2015: 87 · 2014: 42 · 2013: 26 · 2012: 22
- FWCI
- 0.92
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Journal of Bacteriology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Alkaline Phosphatase Research Studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Molecular Research
- Keywords
- iap gene, Escherichia coli, nucleotide sequence, signal peptide, membrane proteins, gene product identification, plasmid genes
- MeSH terms
- Alkaline Phosphatase, Amino Acid Sequence, Bacterial Proteins, Base Sequence, DNA, Bacterial, Escherichia coli, Genes, Bacterial, Isoenzymes, Molecular Sequence Data, Plasmids, Repetitive Sequences, Nucleic Acid, Escherichia coli Proteins
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