Cited by 2,421Bacterial Genetics and BiotechnologyAlkaline Phosphatase Research StudiesBiochemical and Molecular Research

Nucleotide sequence of the iap gene, responsible for alkaline phosphatase isozyme conversion in Escherichia coli, and identification of the gene product

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Ishino, Y, Shinagawa, H, Makino, K, Amemura, M, Nakata, A·1987·Journal of Bacteriology

Abstract

The iap gene in Escherichia coli is responsible for the isozyme conversion of alkaline phosphatase. We analyzed the 1,664-nucleotide sequence of a chromosomal DNA segment that contained the iap gene and its flanking regions. The predicted iap product contained 345 amino acids with an estimated molecular weight of 37,919. The 24-amino-acid sequence at the amino terminus showed features characteristic of a signal peptide. Two proteins of different sizes were identified by the maxicell method, one corresponding to the Iap protein and the other corresponding to the processed product without the signal peptide. Neither the isozyme-converting activity nor labeled Iap proteins were detected in the osmotic-shock fluid of cells carrying a multicopy iap plasmid. The Iap protein seems to be associated with the membrane.

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Crossref · 10.1128/jb.169.12.5429-5433.1987

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Authors
Ishino, Y., Shinagawa, H., Makino, K., Amemura, M., & Nakata, A.
Affiliations
The University of Osaka (JP)
Venue
Journal of Bacteriology
Published
1987-12
Volume
169
Issue
12
Pages
5429-5433
ISSN
  • 0021-9193 (ISSN-L)
  • 1098-5530
OpenAlex
W2118746294
Cited by
2,421
References
26
Citations by year
2026: 120 · 2025: 207 · 2024: 233 · 2023: 255 · 2022: 291 · 2021: 272 · 2020: 205 · 2019: 156 · 2018: 156 · 2017: 140 · 2016: 130 · 2015: 87 · 2014: 42 · 2013: 26 · 2012: 22
FWCI
0.92
Type
journal article
Language
en
Open access
Locations
  • Journal of Bacteriology (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Alkaline Phosphatase Research Studies
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Molecular Research
Keywords
iap gene, Escherichia coli, nucleotide sequence, signal peptide, membrane proteins, gene product identification, plasmid genes
MeSH terms
Alkaline Phosphatase, Amino Acid Sequence, Bacterial Proteins, Base Sequence, DNA, Bacterial, Escherichia coli, Genes, Bacterial, Isoenzymes, Molecular Sequence Data, Plasmids, Repetitive Sequences, Nucleic Acid, Escherichia coli Proteins

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