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Cited by 761Polyomavirus and related diseasesGenomics and Chromatin DynamicsBacterial Genetics and Biotechnology

The SV40 72 base repair repeat has a striking effect on gene expression both in SV40 and other chimeric recombinants

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Moreau, P., Hen, R., Wasylyk, B., Everett, R., Gaub, M.P., Chambon, P.·1981·Nucleic Acids Research
DOI 10.1093/nar/9.22.6047PMC PMC327583
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Abstract

By introduction of recombinant plasmids into monkey CV1 cells, we have unambiguously demonstrated that sequences entirely within the 72 bp repeat, which is located upstream of the SV40 early region, are crucial for T-antigen expression in vivo. We have also shown that a DNA fragment containing the 72 bp repeat, inserted directly before chicken conalbumin or adenovirus-2 major late promoter sequences in chimeric plasmids where these promoters replace that of the SV40 early genes, caused a dramatic increase in the expression of T-antigen in vivo. This effect was independent of the orientation of the 72 bp repeat, but was sensitive to its location within the plasmid, when the 72 bp repeat was separated from the promoter sequences, T-antigen expression was reduced. Insertion of the 72 bp repeat into equivalent plasmids containing no known eukaryotic promoter sequences (plasmids which were not detectably expressed in vivo) gave rise to a measurable, but smaller level of expression. The stimulation of expression by the 72 bp repeat is cis-acting : it required covalent linkage to the recombinant. We discuss the possibility that the 72 bp repeat region in SV40 may act as a bi-directional entry site for RNA polymerase B such that promoter sequences linked to the repeat are more efficiently utilised.

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Crossref · 10.1093/nar/9.22.6047

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  • The SV40 enhancer could activate rabbit or human β-globin genes from varying distances from their promoters, regardless of its orientation.

    “Moreover, this enhancer could activate rabbit or human β-globin genes from varying distances from their promoters, regardless of its orientation [4, 5].”

    From Transcriptional enhancers and their communication with gene promoters (2021) · cites this paper as [5]

  • A 72-bp repeat sequence element in SV40 is an enhancing sequence capable of vastly upregulating gene expression from a plasmid upon transfection into mammalian cells.

    “Subsequent landmark experiments by the groups of Walter Schaffner [4] and Pierre Chambon [5] confirmed that a 72-bp repeat sequence element in SV40 [8, 9] was an “enhancing” sequence, capable of vastly upregulating gene expression from a plasmid upon transfection into mammalian cells.”

    From Transcriptional enhancers and their communication with gene promoters (2021) · cites this paper as [5]

Cited by

Sorted by citations

Authors
Moreau, P., Hen, R., Wasylyk, B., Everett, R., Gaub, M. P., & Chambon, P.
Affiliations
Centre National de la Recherche Scientifique (FR)
ORCID
Venue
Nucleic Acids Research
Publisher
Oxford University Press (OUP)
Published
1981
Volume
9
Issue
22
Pages
6047-6068
ISSN
  • 0305-1048 (ISSN-L)
  • 1362-4962
DOI
10.1093/nar/9.22.6047
PMC
PMC327583
OpenAlex
W2062408930
Cited by
761
References
0
Citations by year
2026: 9 · 2025: 14 · 2024: 10 · 2023: 13 · 2022: 10 · 2021: 16 · 2020: 12 · 2019: 13 · 2018: 16 · 2017: 4 · 2016: 7 · 2015: 10 · 2014: 10 · 2013: 7 · 2012: 6
FWCI
54.33
Type
journal article
Language
en
Open access
Locations
  • Nucleic Acids Research (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Oncology › Polyomavirus and related diseases
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
Keywords
cis-acting enhancer, SV40 early region, chimeric plasmids, RNA polymerase II, transcriptional activation
MeSH terms
Animals, Base Composition, Cell Line, Chlorocebus aethiops, DNA Restriction Enzymes, DNA, Recombinant, DNA, Viral, Fluorescent Antibody Technique, Kidney, Operon, Plasmids, Repetitive Sequences, Nucleic Acid, Simian virus 40, Transcription, Genetic

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