Cited by 13,404RNA and protein synthesis mechanismsProtein Structure and DynamicsDNA and Nucleic Acid Chemistry

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Mfold web server for nucleic acid folding and hybridization prediction

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Zuker, M.·2003·Nucleic Acids Research

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Abstract

The abbreviated name, 'mfold web server', describes a number of closely related software applications available on the World Wide Web (WWW) for the prediction of the secondary structure of single stranded nucleic acids. The objective of this web server is to provide easy access to RNA and DNA folding and hybridization software to the scientific community at large. By making use of universally available web GUIs (Graphical User Interfaces), the server circumvents the problem of portability of this software. Detailed output, in the form of structure plots with or without reliability information, single strand frequency plots and 'energy dot plots', are available for the folding of single sequences. A variety of 'bulk' servers give less information, but in a shorter time and for up to hundreds of sequences at once. The portal for the mfold web server is http://www.bioinfo.rpi.edu/applications/mfold. This URL will be referred to as 'MFOLDROOT'.

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Authors: Zuker, M.
Affiliations: Rensselaer Polytechnic Institute (US)
Corresponding author: Zuker, M.
Venue: Nucleic Acids Research
Publisher: Oxford University Press (OUP)
Published: 2003-07-01
Volume: 31
Issue: 13
Pages: 3406-3415
ISSN: 0305-1048 (ISSN-L)
1362-4962
DOI: 10.1093/nar/gkg595
PMC: PMC169194
OpenAlex: W2141157874
Cited by: 13,404
References: 40
Citations by year: 2026: 320 · 2025: 487 · 2024: 535 · 2023: 536 · 2022: 575 · 2021: 620 · 2020: 615 · 2019: 625 · 2018: 596 · 2017: 653 · 2016: 634 · 2015: 672 · 2014: 681 · 2013: 716 · 2012: 686
FWCI: 151.48
Type: journal article
Language: en
Open access: bronze
doi.org
Locations: Nucleic Acids Research (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
Funders: National Institutes of Health
National Institute of General Medical Sciences (GM54250, R01GM054250)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Structure and Dynamics
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
Keywords: RNA secondary structure prediction, single-stranded nucleic acids, web application, graphical user interface
MeSH terms: Base Sequence, Computer Graphics, DNA, DNA, Single-Stranded, Models, Molecular, Nucleic Acid Conformation, Nucleic Acid Denaturation, Nucleic Acid Hybridization, RNA, Software, Thermodynamics, User-Computer Interface, Internet, Databases, Nucleic Acid

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