The DOI matches its Crossref record; 28 of the 28 references it cites were found in a registry or an index.
Secondary structure effects on DNA hybridization kinetics: a solution versus surface comparison
Open accessAbstract
The hybridization kinetics for a series of designed 25mer probe-target pairs having varying degrees of secondary structure have been measured by UV absorbance and surface plasmon resonance (SPR) spectroscopy in solution and on the surface, respectively. Kinetic rate constants derived from the resultant data decrease with increasing probe and target secondary structure similarly in both solution and surface environments. Specifically, addition of three intramolecular base pairs in the probe and target structure slow hybridization by a factor of two. For individual strands containing four or more intramolecular base pairs, hybridization cannot be described by a traditional two-state model in solution-phase nor on the surface. Surface hybridization rates are also 20- to 40-fold slower than solution-phase rates for identical sequences and conditions. These quantitative findings may have implications for the design of better biosensors, particularly those using probes with deliberate secondary structure.
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Cites
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Dynamic DNA nanotechnology using strand-displacement reactions
Zhang, D. Y., & Seelig, G. (2011) · Nature Chemistry · doi:10.1038/nchem.957 · Cited by 1,785
Control of DNA Strand Displacement Kinetics Using Toehold Exchange
Zhang, D. Y., & Winfree, E. (2009) · Journal of the American Chemical Society · doi:10.1021/ja906987s · Cited by 1,444
On the biophysics and kinetics of toehold-mediated DNA strand displacement
Srinivas, N., Ouldridge, T. E., Šulc, P., Schaeffer, J. M., Yurke, B., Louis, A. A., Doye, J. P. K., & Winfree, E. (2013) · Nucleic Acids Research · doi:10.1093/nar/gkt801 · Cited by 506
Controlling Motion at the Nanoscale: Rise of the Molecular Machines
Abendroth, J. M., Bushuyev, O. S., Weiss, P. S., & Barrett, C. J. (2015) · ACS Nano · doi:10.1021/acsnano.5b03367 · Cited by 447
Solid-state nanopore channels with DNA selectivity
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Metadata
- Authors
- Gao, Y.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 2006-06-28
- Volume
- 34
- Issue
- 11
- Pages
- 3370-3377
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W1990376706
- Cited by
- 335
- References
- 28
- FWCI
- 4.54
- Type
- journal article
- Language
- en
- Open access
- gold
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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