The DOI matches its Crossref record; 31 of the 31 references it cites were found in a registry or an index.
DNA assembler, an in vivo genetic method for rapid construction of biochemical pathways
Open accessAbstract
The assembly of large recombinant DNA encoding a whole biochemical pathway or genome represents a significant challenge. Here, we report a new method, DNA assembler, which allows the assembly of an entire biochemical pathway in a single step via in vivo homologous recombination in Saccharomyces cerevisiae. We show that DNA assembler can rapidly assemble a functional D-xylose utilization pathway (approximately 9 kb DNA consisting of three genes), a functional zeaxanthin biosynthesis pathway (approximately 11 kb DNA consisting of five genes) and a functional combined D-xylose utilization and zeaxanthin biosynthesis pathway (approximately 19 kb consisting of eight genes) with high efficiencies (70-100%) either on a plasmid or on a yeast chromosome. As this new method only requires simple DNA preparation and one-step yeast transformation, it represents a powerful tool in the construction of biochemical pathways for synthetic biology, metabolic engineering and functional genomics studies.
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Cited by
A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
Lee, M. E., DeLoache, W. C., Cervantes, B., & Dueber, J. E. (2015) · ACS Synthetic Biology · doi:10.1021/sb500366v · Cited by 916
Semi-synthetic artemisinin: a model for the use of synthetic biology in pharmaceutical development
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Combinatorial metabolic engineering using an orthogonal tri-functional CRISPR system
Lian, J., HamediRad, M., Hu, S., & Zhao, H. (2017) · Nature Communications · doi:10.1038/s41467-017-01695-x · Cited by 319
Self-citationSynthetic biology of cyanobacteria: unique challenges and opportunities
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Bricks and blueprints: methods and standards for DNA assembly.
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Metadata
- Authors
- Shao, Z., Zhao, H., & Zhao, H.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 2008-12-12
- Volume
- 37
- Issue
- 2
- Pages
- e16-e16
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W1967210731
- Cited by
- 678
- References
- 31
- FWCI
- 5.96
- Type
- journal article
- Language
- en
- Open access
- gold
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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