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A large-scale chemical modification screen identifies design rules to generate siRNAs with high activity, high stability and low toxicity

Bramsen, Jesper B., Laursen, Maria B., Nielsen, Anne F., Hansen, Thomas B., Bus, Claus, Langkjær, Niels, Babu, B. Ravindra, Højland, Torben +23 more·2009·Nucleic Acids Research
DOI 10.1093/nar/gkp106PMC PMC2685080
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  • Chemical modifications that affect the 5' end stability of the siRNA duplex can affect the relative TS of each end and lead to a reversal in siRNA strand selection.

    “In particular, chemical modifications that affect the 5′ end stability of the siRNA duplex can affect the relative TS of each end and lead to a reversal in siRNA strand selection (Bramsen et al., 2009; Bramsen & Kjems, 2013; Ui‐Tei, Nishi, Takahashi, & Nagasawa, 2012).”

    From microRNA strand selection: Unwinding the rules (2021) · cites this paper as Bramsen et al., 2009

  • Modifications to the 3' overhangs can directly affect TS and can influence strand selection.

    “Further, modifications to the 3′ overhangs can also directly affect TS and can influence strand selection (Bramsen et al., 2009; Bramsen & Kjems, 2013; Ui‐Tei et al., 2012).”

    From microRNA strand selection: Unwinding the rules (2021) · cites this paper as Bramsen et al., 2009

  • Modifications that modify the ribose sugar group or substitution modifications to the 2' hydroxyl group of the ribose can affect TS of the siRNA duplex and influence strand selection.

    “Additional modifications that modify the ribose sugar group or substitution modifications to the 2′ hydroxyl group of the ribose can also affect TS of the siRNA duplex and influence strand selection.”

    From microRNA strand selection: Unwinding the rules (2021) · cites this paper as Bramsen et al., 2009

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Research works printed but not linked: 49

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Authors
Bramsen, J. B., Laursen, M. B., Nielsen, A. F., Hansen, T. B., Bus, C., Langkjær, N., Babu, B. R., Højland, T., Abramov, M., Van Aerschot, A., Odadzic, D., Smicius, R., Haas, J., Andree, C., Barman, J., Wenska, M., Srivastava, P., Zhou, C., Honcharenko, D., Hess, S., Müller, E., Bobkov, G. V., Mikhailov, S. N., Fava, E., Meyer, T. F., Chattopadhyaya, J., Zerial, M., Engels, J. W., Herdewijn, P., Wengel, J., & Kjems, J.
Venue
Nucleic Acids Research
Publisher
Oxford University Press (OUP)
Published
2009-03-12
Volume
37
Issue
9
Pages
2867-2881
ISSN
  • 1362-4962
  • 0305-1048
DOI
10.1093/nar/gkp106
PMC
PMC2685080
Cited by
332
References
0
Language
en
Licence
creativecommons.org/licenses/by-nc/2.0/uk/ (vor, 2763 d)

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