Blocking of targeted microRNAs from next-generation sequencing libraries

Roberts, Brian S.; Hardigan, Andrew A.; Kirby, Marie K.; Fitz-Gerald, Meredith B.; Wilcox, C. Mel; Kimberly, Robert P.; Myers, Richard M.

doi:10.1093/nar/gkv724

Cited by 8

The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.

Blocking of targeted microRNAs from next-generation sequencing libraries

Roberts, Brian S., Hardigan, Andrew A., Kirby, Marie K., Fitz-Gerald, Meredith B., Wilcox, C. Mel, Kimberly, Robert P., Myers, Richard M.·2015·Nucleic Acids Research

DOI 10.1093/nar/gkv724PMC PMC4666382

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The hybridization-based approach has a high chance of unspecific hybridization with non-target miRNAs due to the short 12-base complementarity of the stem-loop oligonucleotide.
“Whereas the hybridization-based approach has a high chance of unspecific hybridization with non-target miRNAs due to the short complementarity (12 bases) of the stem-loop oligonucleotide, the CRISPR/Cas9-based method includes additional steps and reagents which increase hands-on-time and costs of the procedure.”
From Depletion of erythropoietic miR-486-5p and miR-451a improves detectability of rare microRNAs in peripheral blood-derived small RNA sequencing libraries (2020) · cites this paper as [8]
Both the hybridization-based and CRISPR/Cas9-based methods have been shown to effectively reduce target miRNA sequences in small RNA libraries.
“Both methods have been shown to effectively reduce target miRNA sequences in small RNA libraries; however, both of them have their own limitations.”
From Depletion of erythropoietic miR-486-5p and miR-451a improves detectability of rare microRNAs in peripheral blood-derived small RNA sequencing libraries (2020) · cites this paper as [8]

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Depletion of erythropoietic miR-486-5p and miR-451a improves detectability of rare microRNAs in peripheral blood-derived small RNA sequencing libraries
Juzenas, S., Lindqvist, C. M., Ito, G., Dolshanskaya, Y., Halfvarson, J., Franke, A., & Hemmrich-Stanisak, G. (2020) · NAR Genomics and Bioinformatics · doi:10.1093/nargab/lqaa008 · Cited by 17

Authors: Roberts, B. S., Hardigan, A. A., Kirby, M. K., Fitz-Gerald, M. B., Wilcox, C. M., Kimberly, R. P., & Myers, R. M.
Venue: Nucleic Acids Research
Publisher: Oxford University Press (OUP)
Published: 2015-07-23
Pages: gkv724
ISSN: 0305-1048
1362-4962
DOI: 10.1093/nar/gkv724
PMC: PMC4666382
Cited by: 8
References: 5
Language: en
Licence: creativecommons.org/licenses/by/4.0/ (vor, 477 d)

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Blocking of targeted microRNAs from next-generation sequencing libraries

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