The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Profiling of 2′-O-Me in human rRNA reveals a subset of fractionally modified positions and provides evidence for ribosome heterogeneity
Open accessAbstract
Ribose methylation is one of the two most abundant modifications in human ribosomal RNA and is believed to be important for ribosome biogenesis, mRNA selectivity and translational fidelity. We have applied RiboMeth-seq to rRNA from HeLa cells for ribosome-wide, quantitative mapping of 2'-O-Me sites and obtained a comprehensive set of 106 sites, including two novel sites, and with plausible box C/D guide RNAs assigned to all but three sites. We find approximately two-thirds of the sites to be fully methylated and the remainder to be fractionally modified in support of ribosome heterogeneity at the level of RNA modifications. A comparison to HCT116 cells reveals similar 2'-O-Me profiles with distinct differences at several sites. This study constitutes the first comprehensive mapping of 2'-O-Me sites in human rRNA using a high throughput sequencing approach. It establishes the existence of a core of constitutively methylated positions and a subset of variable, potentially regulatory positions, and paves the way for experimental analyses of the role of variations in rRNA methylation under different physiological or pathological settings.
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A large nucleolar U3 ribonucleoprotein required for 18S ribosomal RNA biogenesis
Dragon, F., Gallagher, J. E. G., Compagnone-Post, P. A., Mitchell, B. M., Porwancher, K. A., Wehner, K. A., Wormsley, S., Settlage, R. E., Shabanowitz, J., Osheim, Y., et al. (2002) · Nature · doi:10.1038/nature00769 · Cited by 688
Glutamine methylation in histone H2A is an RNA-polymerase-I-dedicated modification
Tessarz, P., Santos-Rosa, H., Robson, S. C., Sylvestersen, K. B., Nelson, C. J., Nielsen, M. L., & Kouzarides, T. (2014) · Nature · doi:10.1038/nature12819 · Cited by 243
Cited by
Sloan, K. E., Warda, A. S., Sharma, S., Entian, K.-D., Lafontaine, D. L. J., & Bohnsack, M. T. (2017) · RNA Biology · doi:10.1080/15476286.2016.1259781 · Cited by 707
Translational control through ribosome heterogeneity and functional specialization
Gay, D. M., Lund, A. H., & Jansson, M. D. (2022) · Trends in Biochemical Sciences · doi:10.1016/j.tibs.2021.07.001 · Cited by 141
Self-citationBox C/D small nucleolar RNA genes and the Prader‐Willi syndrome: a complex interplay
Cavaillé, J. (2017) · WIREs RNA · doi:10.1002/wrna.1417 · Cited by 86
RNA Methylation in ncRNA: Classes, Detection, and Molecular Associations
Romano, G., Veneziano, D., Nigita, G., & Nana-Sinkam, S. P. (2018) · Frontiers in Genetics · doi:10.3389/fgene.2018.00243 · Cited by 49
Ribosomal flavours: An acquired taste for specific mRNAs?
Bates, C., Hubbard, S. J., & Ashe, M. P. (2018) · Biochemical Society Transactions · doi:10.1042/bst20180160 · Cited by 8
Metadata
- Authors
- Krogh, N., Jansson, M. D., Häfner, S. J., Tehler, D., Birkedal, U., Christensen-Dalsgaard, M., Lund, A. H., & Nielsen, H.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 2016-06-01
- Volume
- 44
- Issue
- 16
- Pages
- 7884-7895
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W2408868710
- Cited by
- 262
- References
- 2
- FWCI
- 12.04
- Type
- journal article
- Language
- en
- Open access
- gold
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- Krogh, N., Jansson, M. D., Häfner, S. J., Tehler, D., Birkedal, U., Christensen-Dalsgaard, M., Lund, A. H., & Nielsen, H. (2016). Profiling of 2′-O-Me in human rRNA reveals a subset of fractionally modified positions and provides evidence for ribosome heterogeneity. Nucleic Acids Research. https://doi.org/10.1093/nar/gkw482
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