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Expression Atlas update: from tissues to single cells

Papatheodorou, Irene, Moreno, Pablo, Manning, Jonathan, Fuentes, Alfonso Muñoz-Pomer, George, Nancy, Fexova, Silvie, Fonseca, Nuno A, Füllgrabe, Anja, Green, Matthew, Huang, Ni, Huerta, Laura, Iqbal, Haider, Jianu, Monica, Mohammed, Suhaib, Zhao, Lingyun, Jarnuczak, Andrew F, Jupp, Simon, Marioni, John, Meyer, Kerstin, Petryszak, Robert, Prada Medina, Cesar Augusto, Talavera-López, Carlos, Teichmann, Sarah, Vizcaino, Juan Antonio, Brazma, Alvis·2019·Nucleic Acids Research

Abstract

Expression Atlas is EMBL-EBI’s resource for gene and protein expression. It sources and compiles data on the abundance and localisation of RNA and proteins in various biological systems and contexts and provides open access to this data for the research community. With the increased availability of single cell RNA-Seq datasets in the public archives, we have now extended Expression Atlas with a new added-value service to display gene expression in single cells. Single Cell Expression Atlas was launched in 2018 and currently includes 123 single cell RNA-Seq studies from 12 species. The website can be searched by genes within or across species to reveal experiments, tissues and cell types where this gene is expressed or under which conditions it is a marker gene. Within each study, cells can be visualized using a pre-calculated t-SNE plot and can be coloured by different features or by cell clusters based on gene expression. Within each experiment, there are links to downloadable files, such as RNA quantification matrices, clustering results, reports on protocols and associated metadata, such as assigned cell types.

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Crossref · 10.1093/nar/gkz947

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Figure 1. Screenshot of the ‘Browse Experiments’ page. A user can search through the experiments by species or other keywords, quickly link to their results pages or download raw, normalized data and metadata bundles from one or more studies.
Figure 1. Screenshot of the ‘Browse Experiments’ page. A user can search through the experiments by species or other keywords, quickly link to their results pages or download raw, normalized data and metadata bundles from one or more studies.CC BY · PubMed Central
Figure 2. Search results for gene ASPM, filtered to show datasets where ASPM is expressed in Mus musculus.
Figure 2. Search results for gene ASPM, filtered to show datasets where ASPM is expressed in Mus musculus.CC BY · PubMed Central
Figure 3. The experimental results of the ‘Single cell transcriptome of the human pancreas’ as shown in https://www.ebi.ac.uk/gxa/sc/experiments/E-GEOD-81547/results/tsne?geneId=ENSG00000172023&colourBy=metadata&metadata=inferred_cell_type The results are presented by two identical t-SNE plots where cells are coloured by ‘inferred cell type’ values on the left and the expression levels of gene REG1B (ENSG00000172023) on the right. By viewing these results side by side, a user can easily infer that REG1B is primarily expressed in pancreatic acinar cells.
Figure 3. The experimental results of the ‘Single cell transcriptome of the human pancreas’ as shown in https://www.ebi.ac.uk/gxa/sc/experiments/E-GEOD-81547/results/tsne?geneId=ENSG00000172023&colourBy=metadata&metadata=inferred_cell_type The results are presented by two identical t-SNE plots where cells are coloured by ‘inferred cell type’ values on the left and the expression levels of gene REG1B (ENSG00000172023) on the right. By viewing these results side by side, a user can easily infer that REG1B is primarily expressed in pancreatic acinar cells.CC BY · PubMed Central
Figure 4. Screenshot of a heatmap showing the average expression of ‘marker genes’ within each cell cluster of a single study as shown in https://www.ebi.ac.uk/gxa/sc/experiments/E-GEOD-81547/results/tsne?geneId=ENSG00000172023&colourBy=metadata&metadata=inferred_cell_type&markerGeneK=10.
Figure 4. Screenshot of a heatmap showing the average expression of ‘marker genes’ within each cell cluster of a single study as shown in https://www.ebi.ac.uk/gxa/sc/experiments/E-GEOD-81547/results/tsne?geneId=ENSG00000172023&colourBy=metadata&metadata=inferred_cell_type&markerGeneK=10.CC BY · PubMed Central

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Show all 6 references

Research works printed but not linked: 16

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Metadata

Authors
Papatheodorou, I., Moreno, P., Manning, J., Fuentes, A. M.-P., George, N., Fexova, S., Fonseca, N. A., Füllgrabe, A., Green, M., Huang, N., Huerta, L., Iqbal, H., Jianu, M., Mohammed, S., Zhao, L., Jarnuczak, A. F., Jupp, S., Marioni, J., Meyer, K., Petryszak, R., Prada Medina, C. A., Talavera-López, C., Teichmann, S., Vizcaino, J. A., & Brazma, A.
Affiliations
  • European Molecular Biology Laboratory, European Bioinformatics Institute, EMBL-EBI, Hinxton, UK
  • Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton CB10 1SA, UK
Venue
Nucleic Acids Research
Published
2019-10-30
ISSN
  • 0305-1048
  • 1362-4962
Cited by
224
References
6
Language
en
Funders
  • European Molecular Biology Laboratory
  • Wellcome Trust (108437/Z/15/Z, 208391/Z/17/Z)
  • National Science Foundation (NSF IOS #1127112)
  • BBSRC (BB/P027849/1)

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