Cited by 5,065Molecular Biology Techniques and ApplicationsGene expression and cancer classificationRNA Research and Splicing

Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper – Excel-based tool using pair-wise correlations

Pfaffl, Michael W., Tichopad, Ales, Prgomet, Christian, Neuvians, Tanja P.·2004·Biotechnology Letters

Abstract

The stability of standard gene expression is an elementary prerequisite for internal standardisation of target gene expression data and many so called housekeeping genes with assumed stable expression can exhibit either up- or down-regulation under some experimental conditions. The developed, and herein presented, software called BestKeeper determines the best suited standards, out of ten candidates, and combines them into an index. The index can be compared with further ten target genes to decide, whether they are differentially expressed under an applied treatment. All data processing is based on crossing points. The BestKeeper software tool was validated on four housekeeping genes and 10 members of the somatotropic axis differentially expressed in bovine corpora lutea total RNA. The BestKeeper application and necessary information about data processing and handling can be downloaded on http://www.wzw.tum.de/gene-quantification/bestkeeper.html.

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Crossref · 10.1023/b:bile.0000019559.84305.47

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Authors
Pfaffl, M. W., Tichopad, A., Prgomet, C., & Neuvians, T. P.
Affiliations
Technical University of Munich (DE)
Venue
Biotechnology Letters
Published
2004-03
Volume
26
Issue
6
Pages
509-515
ISSN
  • 0141-5492 (ISSN-L)
  • 1573-6776
OpenAlex
W2099824652
Cited by
5,065
References
19
Citations by year
2026: 180 · 2025: 273 · 2024: 289 · 2023: 287 · 2022: 311 · 2021: 369 · 2020: 391 · 2019: 379 · 2018: 372 · 2017: 333 · 2016: 321 · 2015: 326 · 2014: 282 · 2013: 223 · 2012: 188
FWCI
14.92
Type
journal article
Language
en
Open access
closed
Locations
  • Biotechnology Letters (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Molecular Biology Techniques and Applications
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gene expression and cancer classification
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
Keywords
housekeeping genes, gene expression stability, two-particle correlations, differentially expressed genes, sample integrity, crossing points, somatotropic axis, bovine corpus luteum, gene expression analysis
MeSH terms
Animals, Cattle, Corpus Luteum, Female, Genes, RNA, Software, Gene Expression, Reverse Transcriptase Polymerase Chain Reaction, Gene Expression Profiling

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