The DOI matches its Crossref record; 6 of the 6 references it cites were found in a registry or an index.
Sensitive and specific detection of microRNAs by northern blot analysis using LNA-modified oligonucleotide probes
Open accessAbstract
We describe here a new method for highly efficient detection of microRNAs by northern blot analysis using LNA (locked nucleic acid)-modified oligonucleotides. In order to exploit the improved hybridization properties of LNA with their target RNA molecules, we designed several LNA-modified oligonucleotide probes for detection of different microRNAs in animals and plants. By modifying DNA oligonucleotides with LNAs using a design, in which every third nucleotide position was substituted by LNA, we could use the probes in northern blot analysis employing standard end-labelling techniques and hybridization conditions. The sensitivity in detecting mature microRNAs by northern blots was increased by at least 10-fold compared to DNA probes, while simultaneously being highly specific, as demonstrated by the use of different single and double mismatched LNA probes. Besides being highly efficient as northern probes, the same LNA-modified oligonucleotide probes would also be useful for miRNA in situ hybridization and miRNA expression profiling by LNA oligonucleotide microarrays.
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The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14
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Identification of Novel Genes Coding for Small Expressed RNAs
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The microRNAs of Caenorhabditis elegans
Lim, L. P., Lau, N. C., Weinstein, E. G., Abdelhakim, A., Yekta, S., Rhoades, M. W., Burge, C. B., & Bartel, D. P. (2003) · Genes & Development · doi:10.1101/gad.1074403 · Cited by 1,259
Cited by
MicroRNA Expression in Zebrafish Embryonic Development
Wienholds, E., Kloosterman, W. P., Miska, E., Alvarez-Saavedra, E., Berezikov, E., de Bruijn, E., Horvitz, H. R., Kauppinen, S., & Plasterk, R. H. A. (2005) · Science · doi:10.1126/science.1114519 · Cited by 1,551
Modulating Hox gene functions during animal body patterning
Pearson, J. C., Lemons, D., & McGinnis, W. (2005) · Nature Reviews Genetics · doi:10.1038/nrg1726 · Cited by 879
MicroRNA as Biomarkers and Diagnostics
Wang, J., Chen, J., & Sen, S. (2016) · Journal of Cellular Physiology · doi:10.1002/jcp.25056 · Cited by 793
A cAMP-response element binding protein-induced microRNA regulates neuronal morphogenesis
Vo, N., Klein, M. E., Varlamova, O., Keller, D. M., Yamamoto, T., Goodman, R. H., & Impey, S. (2005) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0508448102 · Cited by 696
MicroRNA detection by northern blotting using locked nucleic acid probes
Várallyay, É., Burgyán, J., & Havelda, Z. (2008) · Nature Protocols · doi:10.1038/nprot.2007.528 · Cited by 677
Metadata
- Authors
- Valoczi, A.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 2004-12-14
- Volume
- 32
- Issue
- 22
- Pages
- e175-e175
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W2001911979
- Cited by
- 866
- References
- 6
- FWCI
- 10.28
- Type
- journal article
- Language
- en
- Open access
- bronze
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
