The DOI matches its Crossref record; 39 of the 39 references it cites were found in a registry or an index.
Three SnRK2 Protein Kinases are the Main Positive Regulators of Abscisic Acid Signaling in Response to Water Stress in Arabidopsis
Abstract
Responses to water stress are thought to be mediated by transcriptional regulation of gene expression via reversible protein phosphorylation events. Previously, we reported that bZIP (basic-domain leucine zipper)-type AREB/ABF (ABA-responsive element-binding protein/factor) transcription factors are involved in ABA signaling under water stress conditions in Arabidopsis. The AREB1 protein is phosphorylated in vitro by ABA-activated SNF1-related protein kinase 2s (SnRK2s) such as SRK2D/SnRK2.2, SRK2E/SnRK2.6 and SRK2I/SnRK2.3 (SRK2D/E/I). Consistent with this, we now show that SRK2D/E/I and AREB1 co-localize and interact in nuclei in planta. Our results show that unlike srk2d, srk2e and srk2i single and double mutants, srk2d srk2e srk2i (srk2d/e/i) triple mutants exhibit greatly reduced tolerance to drought stress and highly enhanced insensitivity to ABA. Under water stress conditions, ABA- and water stress-dependent gene expression, including that of transcription factors, is globally and drastically impaired, and jasmonic acid (JA)-responsive and flowering genes are up-regulated in srk2d/e/i triple mutants, but not in other single and double mutants. The down-regulated genes in srk2d/e/i and areb/abf triple mutants largely overlap in ABA-dependent expression, supporting the view that SRK2D/E/I regulate AREB/ABFs in ABA signaling in response to water stress. Almost all dehydration-responsive LEA (late embryogenesis abundant) protein genes and group-A PP2C (protein phosphatase 2C) genes are strongly down-regulated in the srk2d/e/i triple mutants. Further, our data show that these group-A PP2Cs, such as HAI1 and ABI1, interact with SRK2D. Together, our results indicate that SRK2D/E/I function as main positive regulators, and suggest that ABA signaling is controlled by the dual modulation of SRK2D/E/I and group-A PP2Cs.
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Cites
Abscisic Acid Inhibits Type 2C Protein Phosphatases via the PYR/PYL Family of START Proteins
Park, S.-Y., Fung, P., Nishimura, N., Jensen, D. R., Fujii, H., Zhao, Y., Lumba, S., Santiago, J., Rodrigues, A., Chow, T.-F. F., et al. (2009) · Science · doi:10.1126/science.1173041 · Cited by 2,974
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Self-citationDrought and Salt Tolerance in Plants
Bartels, D., & Sunkar, R. (2005) · Critical Reviews in Plant Sciences · doi:10.1080/07352680590910410 · Cited by 2,615
GENEVESTIGATOR. Arabidopsis Microarray Database and Analysis Toolbox
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Regulators of PP2C Phosphatase Activity Function as Abscisic Acid Sensors
Ma, Y., Szostkiewicz, I., Korte, A., Moes, D., Yang, Y., Christmann, A., & Grill, E. (2009) · Science · doi:10.1126/science.1172408 · Cited by 2,459
Abscisic Acid Signaling in Seeds and Seedlings
Finkelstein, R. R., Gampala, S. S. L., & Rock, C. D. (2002) · The Plant Cell · doi:10.1105/tpc.010441 · Cited by 2,232
Fujita, M., Fujita, Y., Noutoshi, Y., Takahashi, F., Narusaka, Y., Yamaguchi-Shinozaki, K., & Shinozaki, K. (2006) · Current Opinion in Plant Biology · doi:10.1016/j.pbi.2006.05.014 · Cited by 1,834
Self-citationWalter, M., Chaban, C., Schütze, K., Batistic, O., Weckermann, K., Näke, C., Blazevic, D., Grefen, C., Schumacher, K., Oecking, C., et al. (2004) · The Plant Journal · doi:10.1111/j.1365-313x.2004.02219.x · Cited by 1,728
FD, a bZIP Protein Mediating Signals from the Floral Pathway Integrator FT at the Shoot Apex
Abe, M., Kobayashi, Y., Yamamoto, S., Daimon, Y., Yamaguchi, A., Ikeda, Y., Ichinoki, H., Notaguchi, M., Goto, K., & Araki, T. (2005) · Science · doi:10.1126/science.1115983 · Cited by 1,654
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Cited by
Raghavendra, A. S., Gonugunta, V. K., Christmann, A., & Grill, E. (2010) · Trends in Plant Science · doi:10.1016/j.tplants.2010.04.006 · Cited by 1,309
Abscisic Acid Synthesis and Response
Finkelstein, R. (2013) · The Arabidopsis Book · doi:10.1199/tab.0166 · Cited by 1,154
Yoshida, T., Fujita, Y., Sayama, H., Kidokoro, S., Maruyama, K., Mizoi, J., Shinozaki, K., & Yamaguchi-Shinozaki, K. (2010) · The Plant Journal · doi:10.1111/j.1365-313x.2009.04092.x · Cited by 1,119
Self-citationMolecular Basis of the Core Regulatory Network in ABA Responses: Sensing, Signaling and Transport
Umezawa, T., Nakashima, K., Miyakawa, T., Kuromori, T., Tanokura, M., Shinozaki, K., & Yamaguchi-Shinozaki, K. (2010) · Plant and Cell Physiology · doi:10.1093/pcp/pcq156 · Cited by 929
Self-citationNakashima, K., Yamaguchi-Shinozaki, K., & Shinozaki, K. (2014) · Frontiers in Plant Science · doi:10.3389/fpls.2014.00170 · Cited by 891
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Metadata
- Authors
- Fujita, Y., Nakashima, K., Yoshida, T., Katagiri, T., Kidokoro, S., Kanamori, N., Umezawa, T., Fujita, M., Maruyama, K., Ishiyama, K., Kobayashi, M., Nakasone, S., Yamada, K., Ito, T., Shinozaki, K., & Yamaguchi-Shinozaki, K.
- Venue
- Plant and Cell Physiology
- Publisher
- Oxford University Press (OUP)
- Published
- 2009-09-24
- Volume
- 50
- Issue
- 12
- Pages
- 2123-2132
- ISSN
- 0032-0781 (ISSN-L)
- 1471-9053
- OpenAlex
- W2165829183
- Cited by
- 730
- References
- 39
- FWCI
- 35.52
- Type
- journal article
- Language
- en
- Open access
- closed
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- Fujita, Y., Nakashima, K., Yoshida, T., Katagiri, T., Kidokoro, S., Kanamori, N., Umezawa, T., Fujita, M., Maruyama, K., Ishiyama, K., Kobayashi, M., Nakasone, S., Yamada, K., Ito, T., Shinozaki, K., & Yamaguchi-Shinozaki, K. (2009). Three SnRK2 Protein Kinases are the Main Positive Regulators of Abscisic Acid Signaling in Response to Water Stress in Arabidopsis. Plant and Cell Physiology. https://doi.org/10.1093/pcp/pcp147
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