Integrated printed BDNF-stimulated HUCMSCs-derived exosomes/collagen/chitosan biological scaffolds with 3D printing technology promoted the remodelling of neural networks after traumatic brain injury
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Abstract The restoration of nerve dysfunction after traumatic brain injury (TBI) faces huge challenges due to the limited self-regenerative abilities of nerve tissues. In situ inductive recovery can be achieved utilizing biological scaffolds combined with endogenous human umbilical cord mesenchymal stem cells (HUCMSCs)-derived exosomes (MExos). In this study, brain-derived neurotrophic factor-stimulated HUCMSCs-derived exosomes (BMExos) were composited with collagen/chitosan by 3D printing technology. 3D-printed collagen/chitosan/BMExos (3D-CC-BMExos) scaffolds have excellent mechanical properties and biocompatibility. Subsequently, in vivo experiments showed that 3D-CC-BMExos therapy could improve the recovery of neuromotor function and cognitive function in a TBI model in rats. Consistent with the behavioural recovery, the results of histomorphological tests showed that 3D-CC-BMExos therapy could facilitate the remodelling of neural networks, such as improving the regeneration of nerve fibres, synaptic connections and myelin sheaths, in lesions after TBI.
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Exosomes from BDNF-stimulated HUCMSCs incorporated into collagen/chitosan scaffolds via 3D printing significantly improved neuromotor and cognitive abilities in a rat TBI model.
“In a pioneering study by Liu et al., exosomes from BDNF-stimulated HUCMSCs (BMExos) were incorporated into collagen/chitosan scaffolds via 3D printing [290]. These scaffolds, which have outstanding mechanical properties and biocompatibility, were shown in vivo to significantly improve neuromotor and cognitive abilities in a rat TBI model”
From Extracellular vesicles as nanotheranostic platforms for targeted neurological disorder interventions (2024) · cites this paper as [290]
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- Authors
- Liu, X., Zhang, J., Cheng, X., Liu, P., Feng, Q., Wang, S., Li, Y., Gu, H., Zhong, L., Chen, M., & Zhou, L.
- Affiliations
- Sichuan University (CN)
- Chinese People's Armed Police Force (CN)
- Chinese People's Liberation Army (CN)
- First Affiliated Hospital of Chengdu Medical College (CN)
- Xinjiang Medical University (CN)
- Corresponding author
- Zhong, Lin
- Chen, Miao
- Zhou, Liangxue
- Venue
- Regenerative Biomaterials
- Publisher
- Oxford University Press (OUP)
- Published
- 2022-10-26
- Volume
- 10
- Pages
- rbac085-rbac085
- ISSN
- 2056-3418 (ISSN-L)
- 2056-3426
- PMC
- PMC9847532
- OpenAlex
- W4307303101
- Cited by
- 58
- References
- 51
- Citations by year
- 2026: 16 · 2025: 7 · 2024: 24 · 2023: 11
- FWCI
- 5.53
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Regenerative Biomaterials (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- National Major Scientific and Technological Special Project for Significant New Drugs Development (2015ZX09102010)
- Topics
- Health Sciences › Medicine › Neurology › Traumatic Brain Injury and Neurovascular Disturbances
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Nerve injury and regeneration
- Keywords
- traumatic brain injury, exosomes, human umbilical cord mesenchymal stem cells, brain-derived neurotrophic factor, collagen, chitosan, 3D-printed scaffolds, cognitive recovery, myelin sheath regeneration, synaptic connectivity, in situ tissue engineering
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