Separate and Combined Disruptions of Two Exo-β-1,3-Glucanase Genes Decrease the Efficiency of Pichia anomala (Strain K) Biocontrol Against Botrytis cinerea on Apple
Open accessAbstract
The modes of action of the antagonistic yeast Pichia anomala (strain K) have been studied; however, thus far, there has been no clear demonstration of the involvement of exo-β-1,3-glucanase in determining the level of protection against Botrytis cinerea afforded by this biocontrol agent on apple. In the present study, the exo-β-1,3-glucanase-encoding genes PAEXG1 and PAEXG2, previously sequenced from the strain K genome, were separately and sequentially disrupted. Transfer of the URA3-Blaster technique to strain K, allowing multiple use of URA3 marker gene, first was validated by efficient inactivation of the PaTRP1 gene and recovery of a double auxotrophic strain (uracil and tryptophan). The PAEXG1 and PAEXG2 genes then were inactivated separately and sequentially with the unique URA3 marker gene. The resulting mutant strains showed a significantly reduced efficiency of biocontrol of B. cinerea when applied to wounded apple fruit, the calculated protection level dropping from 71% (parental strain) to 8% (mutated strain) under some experimental conditions. This suggests that exo-β-1,3-glucanases play a role in the biological control of B. cinerea on apple. Furthermore, biological control experiments carried out in this study underline the complexity of the host-antagonist-pathogen interaction. Two experimental parameters (yeast inoculum concentration and physiological stage of the fruit) were found to influence dramatically the protection level. Results also suggest that, under some conditions, the contribution of exo-β-1,3-glucanase to biological control may be masked by other modes of action, such as competition.
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In Wickerhamomyces anomalus, the deletion of the two exo-β-glucanases (PaEXG1 and PaEXG2) significantly reduced biocontrol activity on fruits against Botrytis cinerea, while the single deletion of PaEXG2 did not reduce biocontrol performance.
“In Wickerhamomyces anomalus (Pichia anomala), the deletion of the two exo-β-glucanases (PaEXG1 and PaEXG2) significantly reduced biocontrol activity on fruits against Botrytis cinerea (Friel et al. 2007), while the single deletion of PaEXG2 did not reduce biocontrol performance (Grevesse et al. 2003).”
From Biocontrol yeasts: mechanisms and applications (2019) · cites this paper as Friel et al. 2007
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- Authors
- Friel, D., Pessoa, N. M. G., Vandenbol, M., & Jijakli, M. H.
- Affiliations
- Gembloux Agro-Bio Tech (BE)
- University of Liège (BE)
- Venue
- Molecular Plant-Microbe Interactions®
- Publisher
- Scientific Societies
- Published
- 2007-04
- Volume
- 20
- Issue
- 4
- Pages
- 371-379
- ISSN
- 0894-0282 (ISSN-L)
- 1943-7706
- OpenAlex
- W1975072368
- Cited by
- 62
- References
- 20
- Citations by year
- 2026: 3 · 2025: 1 · 2024: 4 · 2023: 3 · 2022: 3 · 2021: 4 · 2020: 3 · 2019: 2 · 2018: 3 · 2017: 2 · 2016: 3 · 2015: 3 · 2014: 8 · 2013: 2 · 2012: 4
- FWCI
- 3.37
- Type
- journal article
- Language
- en
- Open access
- green
- orbi.uliege.be
- Licence
- other-oa
- Locations
- Molecular Plant-Microbe Interactions (publishedVersion)
- PubMed (publishedVersion)
- Open Repository and Bibliography (University of Liège) (submittedVersion, other-oa)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- Funders
- Fonds De La Recherche Scientifique - FNRS
- Conselho Nacional de Desenvolvimento Científico e Tecnológico
- Topics
- Life Sciences › Agricultural and Biological Sciences › Ecology, Evolution, Behavior and Systematics › Fungal Plant Pathogen Control
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant-Microbe Interactions and Immunity
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Plant Pathogens and Fungal Diseases
- Keywords
- Wickerhamomyces anomalus, Botrytis cinerea, exo-β-1,3-glucanase, gene disruption, biological control, wound infection, competition (biology), antifungal enzymes
- MeSH terms
- Food Microbiology, Genes, Fungal, Pest Control, Biological, Pichia, Plant Diseases, Mutagenesis, Botrytis, Gene Silencing, Malus, Glucan 1,3-beta-Glucosidase
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