The DOI matches its Crossref record; 22 of the 22 references it cites were found in a registry or an index.
Identification of a specific sperm nuclei selenoenzyme necessary for protamine thiol cross‐linking during sperm maturation
Abstract
A 34 kD selenoprotein purified from rat testis was identified as a specific sperm nuclei glutathione peroxidase (snGPx) with similar properties to phospholipid hydroperoxide glutathione peroxidase (PHGPx). The determination of its primary structure by analysis of its first N‐terminal amino acids, database search, polymerase chain reaction, and sequencing of the cDNA showed that it differs from PHGPx in its N‐terminal sequence. This sequence, which is encoded for by an alternative exon in the first intron of the PHGPx gene, shows more than 50% homology to the protamine sequences and contains a nuclear localization signal. In rats, snGPx is highly expressed in the nuclei of the late spermatids where it is the only selenoprotein present. Its appearance coincides with the reorganization of DNA, which leads to highly condensed chromatin stabilized by cross‐linked protamine thiols. In selenium‐depleted rats where the concentration of snGPx had decreased to one‐third of the normal level, chromatin condensation was severely disturbed. We provided evidence that snGPx acts as a protamine thiol peroxidase responsible for disulfide cross‐linking by reduction of reactive oxygen species. Its dual function in chromatin condensation and the protection of sperm DNA against oxidation is necessary to ensure male fertility and sperm quality.
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Cites
Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease
Chirgwin, J. M., Przybyla, A. E., MacDonald, R. J., & Rutter, W. J. (1979) · Biochemistry · doi:10.1021/bi00591a005 · Cited by 21,725
Selenium: Biochemical Role as a Component of Glutathione Peroxidase
Rotruck, J. T., Pope, A. L., Ganther, H. E., Swanson, A. B., Hafeman, D. G., & Hoekstra, W. G. (1973) · Science · doi:10.1126/science.179.4073.588 · Cited by 7,809
The selenoenzyme phospholipid hydroperoxide glutathione peroxidase
Ursini, F., Maiorino, M., & Gregolin, C. (1985) · Biochimica et Biophysica Acta (BBA) - General Subjects · doi:10.1016/0304-4165(85)90182-5 · Cited by 990
Dual Function of the Selenoprotein PHGPx During Sperm Maturation
Ursini, F., Heim, S., Kiess, M., Maiorino, M., Roveri, A., Wissing, J., & Flohé, L. (1999) · Science · doi:10.1126/science.285.5432.1393 · Cited by 903
Chu, F. F., Doroshow, J. H., & Esworthy, R. S. (1993) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)53812-6 · Cited by 553
Evidence for specific selenium target tissues and new biologically important selenoproteins
Behne, D., Hilmert, H., Scheid, S., Gessner, H., & Elger, W. (1988) · Biochimica et Biophysica Acta (BBA) - General Subjects · doi:10.1016/0304-4165(88)90123-7 · Cited by 467
Self-citationThe Amoroso Lecture The human spermatozoon – a cell in crisis?
Aitken, R. J. (1999) · Reproduction · doi:10.1530/jrf.0.1150001 · Cited by 420
Takahashi, K., Avissar, N., Whitin, J., & Cohen, H. (1987) · Archives of Biochemistry and Biophysics · doi:10.1016/0003-9861(87)90624-2 · Cited by 411
Hughes, C. M., Lewis, S. E. M., McKelvey-Martin, V. J., & Thompson, W. (1996) · Molecular Human Reproduction · doi:10.1093/molehr/2.8.613 · Cited by 395
Effects of selenium deficiency on testicular morphology and function in rats
Behne, D., Weiler, H., & Kyriakopoulos, A. (1996) · Reproduction · doi:10.1530/jrf.0.1060291 · Cited by 247
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Nordberg, J., & Arnér, E. S. J. (2001) · Free Radical Biology and Medicine · doi:10.1016/s0891-5849(01)00724-9 · Cited by 2,662
Targeting Ferroptosis as a Vulnerability in Cancer
Lei, G., Zhuang, L., & Gan, B. (2022) · Nature reviews. Cancer · doi:10.1038/s41568-022-00459-0 · Cited by 2,252
Brigelius-Flohé, R., & Maiorino, M. (2013) · Biochimica et Biophysica Acta (BBA) - General Subjects · doi:10.1016/j.bbagen.2012.11.020 · Cited by 1,904
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Tremellen, K. (2008) · Human Reproduction Update · doi:10.1093/humupd/dmn004 · Cited by 1,507
Ferroptosis in cancer: from molecular mechanisms to therapeutic strategies
Zhou, Q., Meng, Y., Li, D., Yao, L., Le, J., Liu, Y., Sun, Y., Zeng, F., Chen, X., & Deng, G. (2024) · Signal Transduction and Targeted Therapy · doi:10.1038/s41392-024-01769-5 · Cited by 890
Metadata
- Authors
- Pfeifer, H., Conrad, M., Roethlein, D., Kyriakopoulos, A., Brielmeier, M., Bornkamm, G. W., & Behne, D.
- Venue
- The FASEB Journal
- Publisher
- Wiley
- Published
- 2001-03-05
- Volume
- 15
- Issue
- 7
- Pages
- 1236-1238
- ISSN
- 0892-6638 (ISSN-L)
- 1530-6860
- OpenAlex
- W1993944463
- Cited by
- 281
- References
- 22
- FWCI
- 17.17
- Type
- journal article
- Language
- en
- Open access
- closed
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- Pfeifer, H., Conrad, M., Roethlein, D., Kyriakopoulos, A., Brielmeier, M., Bornkamm, G. W., & Behne, D. (2001). Identification of a specific sperm nuclei selenoenzyme necessary for protamine thiol cross‐linking during sperm maturation. The FASEB Journal. https://doi.org/10.1096/fj.00-0655fje
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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