The DOI matches its Crossref record; 34 of the 34 references it cites were found in a registry or an index.
Reactive glial cells: increased stiffness correlates with increased intermediate filament expression
Abstract
Increased stiffness of reactive glial cells may impede neurite growth and contribute to the poor regenerative capabilities of the mammalian central nervous system. We induced reactive gliosis in rodent retina by ischemia-reperfusion and assessed intermediate filament (IF) expression and the viscoelastic properties of dissociated single glial cells in wild-type mice, mice lacking glial fibrillary acidic protein and vimentin (GFAP(-/-)Vim(-/-)) in which glial cells are consequently devoid of IFs, and normal Long-Evans rats. In response to ischemia-reperfusion, glial cells stiffened significantly in wild-type mice and rats but were unchanged in GFAP(-/-)Vim(-/-) mice. Cell stiffness (elastic modulus) correlated with the density of IFs. These results support the hypothesis that rigid glial scars impair nerve regeneration and that IFs are important determinants of cellular viscoelasticity in reactive glia. Thus, therapeutic suppression of IF up-regulation in reactive glial cells may facilitate neuroregeneration.
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“In several models of acute central nervous system injury, deletion of the genes encoding these proteins suppresses morphological changes associated with astrocyte activation (22, 23, 26, 30, 32, 36).”
From Attenuating astrocyte activation accelerates plaque pathogenesis in APP/PS1 mice (2013) · cites this paper as [26]
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Cites
Müller cells in the healthy and diseased retina
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Astrocyte activation and reactive gliosis
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Self-citationNeurite branching on deformable substrates
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“IFs and IF proteins are commonly used as markers of normal and reactive glial cells (9, 24, 27), but their physiological and pathophysiological functions are poorly understood (10).”
BackgroundSelf-citationViscoelastic properties of individual glial cells and neurons in the CNS
Lu, Y., Franze, K., Seifert, G., Steinhäuser, C., Kirchhoff, F., Wolburg, H., Guck, J. R., Janmey, P. A., Wei, E.-Q., Käs, J. A., et al. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0606150103 · Cited by 580
Self-citationScanning Probe-Based Frequency-Dependent Microrheology of Polymer Gels and Biological Cells
Mahaffy, R. E., Shih, C.-K. K., MacKintosh, F. C., & Käs, J. A. (2000) · Physical Review Letters · doi:10.1103/physrevlett.85.880 · Cited by 509
Self-citationMahaffy, R. E., Park, S., Gerde, E., Käs, J. A., & Shih, C.-K. K. (2004) · Biophysical Journal · doi:10.1016/s0006-3495(04)74245-9 · Cited by 460
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Self-citationAstrocytes and the regulation of cerebral blood flow
Koehler, R. C., Roman, R. J., & Harder, D. R. (2009) · Trends in Neurosciences · doi:10.1016/j.tins.2008.11.005 · Cited by 439
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Metadata
- Authors
- Lu, Y., Iandiev, I., Hollborn, M., Körber, N., Ulbricht, E., Hirrlinger, P. G., Pannicke, T., Wei, E., Bringmann, A., Wolburg, H., Wilhelmsson, U., Pekny, M., Wiedemann, P., Reichenbach, A., & Käs, J. A.
- Affiliations
- Zhejiang University (CN)
- Leipzig University (DE)
- University of Tübingen (DE)
- University of Gothenburg (SE)
- Corresponding author
- Reichenbach, Andreas
- ORCID
- Venue
- The FASEB Journal
- Publisher
- Wiley
- Published
- 2010-10-25
- Volume
- 25
- Issue
- 2
- Pages
- 624-631
- ISSN
- 0892-6638 (ISSN-L)
- 1530-6860
- OpenAlex
- W1977341002
- Cited by
- 172
- References
- 34
- Citations by year
- 2026: 3 · 2025: 11 · 2024: 7 · 2023: 6 · 2022: 7 · 2021: 15 · 2020: 15 · 2019: 14 · 2018: 11 · 2017: 12 · 2016: 10 · 2015: 17 · 2014: 10 · 2013: 19 · 2012: 12
- FWCI
- 4.76
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- The FASEB Journal (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Deutsche Forschungsgemeinschaft (SPP1172)
- National Natural Science Foundation of China (30700150)
- Deutscher Akademischer Austauschdienst (Y2080105)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Skin and Cellular Biology Research
- Health Sciences › Medicine › Ophthalmology › Glaucoma and retinal disorders
- Keywords
- reactive glial cells, cell stiffness, intermediate filaments, glial fibrillary acidic protein, vimentin, reactive gliosis, retinal ischemia-reperfusion, viscoelasticity, glial scar, nerve regeneration
- MeSH terms
- Animals, Biomechanical Phenomena, Gene Expression Regulation, Glial Fibrillary Acidic Protein, Gliosis, Intermediate Filaments, Nerve Tissue Proteins, Neuroglia, Vimentin, Reperfusion Injury, Mice, Knockout, Rats, Long-Evans, Mice, Rats
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