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Reactive glial cells: increased stiffness correlates with increased intermediate filament expression
Abstract
Increased stiffness of reactive glial cells may impede neurite growth and contribute to the poor regenerative capabilities of the mammalian central nervous system. We induced reactive gliosis in rodent retina by ischemia-reperfusion and assessed intermediate filament (IF) expression and the viscoelastic properties of dissociated single glial cells in wild-type mice, mice lacking glial fibrillary acidic protein and vimentin (GFAP(-/-)Vim(-/-)) in which glial cells are consequently devoid of IFs, and normal Long-Evans rats. In response to ischemia-reperfusion, glial cells stiffened significantly in wild-type mice and rats but were unchanged in GFAP(-/-)Vim(-/-) mice. Cell stiffness (elastic modulus) correlated with the density of IFs. These results support the hypothesis that rigid glial scars impair nerve regeneration and that IFs are important determinants of cellular viscoelasticity in reactive glia. Thus, therapeutic suppression of IF up-regulation in reactive glial cells may facilitate neuroregeneration.
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From Attenuating astrocyte activation accelerates plaque pathogenesis in APP/PS1 mice (2013) · cites this paper as [26]
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Metadata
- Authors
- Lu, Y., Iandiev, I., Hollborn, M., Körber, N., Ulbricht, E., Hirrlinger, P. G., Pannicke, T., Wei, E., Bringmann, A., Wolburg, H., Wilhelmsson, U., Pekny, M., Wiedemann, P., Reichenbach, A., & Käs, J. A.
- Affiliations
- Zhejiang University (CN)
- Leipzig University (DE)
- University of Tübingen (DE)
- University of Gothenburg (SE)
- Corresponding author
- Reichenbach, Andreas
- ORCID
- Venue
- The FASEB Journal
- Publisher
- Wiley
- Published
- 2010-10-25
- Volume
- 25
- Issue
- 2
- Pages
- 624-631
- ISSN
- 0892-6638 (ISSN-L)
- 1530-6860
- OpenAlex
- W1977341002
- Cited by
- 172
- References
- 34
- Citations by year
- 2026: 3 · 2025: 11 · 2024: 7 · 2023: 6 · 2022: 7 · 2021: 15 · 2020: 15 · 2019: 14 · 2018: 11 · 2017: 12 · 2016: 10 · 2015: 17 · 2014: 10 · 2013: 19 · 2012: 12
- FWCI
- 4.76
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- The FASEB Journal (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Deutsche Forschungsgemeinschaft (SPP1172)
- National Natural Science Foundation of China (30700150)
- Deutscher Akademischer Austauschdienst (Y2080105)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Skin and Cellular Biology Research
- Health Sciences › Medicine › Ophthalmology › Glaucoma and retinal disorders
- Keywords
- reactive glial cells, cell stiffness, intermediate filaments, glial fibrillary acidic protein, vimentin, reactive gliosis, retinal ischemia-reperfusion, viscoelasticity, glial scar, nerve regeneration
- MeSH terms
- Animals, Biomechanical Phenomena, Gene Expression Regulation, Glial Fibrillary Acidic Protein, Gliosis, Intermediate Filaments, Nerve Tissue Proteins, Neuroglia, Vimentin, Reperfusion Injury, Mice, Knockout, Rats, Long-Evans, Mice, Rats
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