Cited by 37Axon Guidance and Neuronal SignalingCellular transport and secretionProtein Kinase Regulation and GTPase Signaling

FE65 interacts with ADP-ribosylation factor 6 to promote neurite outgrowth

Open access
Cheung, Hei Nga Maggie, Dunbar, Charlotte, Mórotz, Gábor M., Cheng, Wai Hang, Chan, Ho Yin Edwin, Miller, Christopher C. J., Lau, Kwok‐Fai·2014·The FASEB Journal

Abstract

FE65 is an adaptor protein that binds to the amyloid precursor protein (APP). As such, FE65 has been implicated in the pathogenesis of Alzheimer's disease. In addition, evidence suggests that FE65 is involved in brain development. It is generally believed that FE65 participates in these processes by recruiting various interacting partners to form functional complexes. Here, we show that via its first phosphotyrosine binding (PTB) domain, FE65 binds to the small GTPase ADP‐ribosylation factor 6 (ARF6). FE65 preferentially binds to ARF6‐GDP, and they colocalize in neuronal growth cones. Interestingly, FE65 stimulates the activation of both ARF6 and its downstream GTPase Rac1, a regulator of actin dynamics, and functions in growth cones to stimulate neurite outgrowth. We show that transfection of FE65 and/or ARF6 promotes whereas small interfering RNA knockdown of FE65 or ARF6 inhibits neurite outgrowth in cultured neurons as compared to the mock‐transfected control cells. Moreover, knockdown of ARF6 attenuates FE65 stimulation of neurite outgrowth and defective neurite outgrowth seen in FE65‐deficient neurons is partially corrected by ARF6 overexpression. Notably, the stimulatory effect of FE65 and ARF6 on neurite outgrowth is abrogated either by dominant‐negative Rac1 or knockdown of Rac1. Thus, we identify FE65 as a novel regulator of neurite outgrowth via controlling ARF6‐Rac1 signaling.—Cheung, H. N., Dunbar, C., Mórotz, G. M., Cheng, W. H., Chan, H. Y., Miller, C. C., Lau, K.‐.F. FE65 interacts with ADP‐ribosylation factor 6 to promote neurite outgrowth. FASEB J . 28, 337–349 (2014). www.fasebj.org

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1096/fj.13-232694

Cites

91 of 91 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • Apbb1 is an adapter protein that functions in neurite outgrowth and synaptic plasticity, processes linked to neurological disorders including ASD.

    “Apbb1 is an adapter protein that functions in neurite outgrowth (Cheung et al., 2014; Ikin et al., 2007) and synaptic plasticity (Sabo et al., 2003), processes that have been linked to neurological disorders including ASD (Hussman et al., 2011)”

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Cites

Flagged first, then by citations

2 of the 93 references couldn’t be matched to a known paper.

Cited by

Sorted by citations

Authors
Cheung, H. N. M., Dunbar, C., Mórotz, G. M., Cheng, W. H., Chan, H. Y. E., Miller, C. C. J., & Lau, K.
Affiliations
  • Chinese University of Hong Kong (HK)
  • King's College London (GB)
Corresponding author
Lau, Kwok‐Fai
Venue
The FASEB Journal
Publisher
Wiley
Published
2013-09-20
Volume
28
Issue
1
Pages
337-349
ISSN
  • 0892-6638 (ISSN-L)
  • 1530-6860
OpenAlex
W2034868654
Cited by
37
References
93
Citations by year
2026: 1 · 2025: 1 · 2024: 1 · 2023: 2 · 2022: 3 · 2021: 2 · 2020: 3 · 2019: 1 · 2018: 2 · 2017: 8 · 2016: 2 · 2015: 7 · 2014: 4
FWCI
2.10
Type
journal article
Language
en
Open access
Locations
  • The FASEB Journal (publishedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
  • Research Portal (King's College London) (submittedVersion)
Funders
  • Wellcome Trust
  • Medical Research Council
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Axon Guidance and Neuronal Signaling
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
Keywords
Fe65, ARF6, neurite outgrowth, Rac1, phosphotyrosine binding domain, small GTPase activation, growth cones, amyloid precursor protein, Alzheimer's disease, actin dynamics, neuronal development
MeSH terms
ADP-Ribosylation Factor 6, Animals, Cells, Cultured, Cricetulus, Nerve Tissue Proteins, Nuclear Proteins, Protein Binding, CHO Cells, Neurites, ADP-Ribosylation Factors, Two-Hybrid System Techniques, Immunoprecipitation, Rats

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review