Non-synaptic and dendritic exocytosis from dense-cored vesicles in the suprachiasmatic nucleus
Abstract
The sites of exocytosis by dense-cored vesicles (DCVs) from neurones in the rat suprachiasmatic nucleus (SCN) were studied at the ultrastructural level. The tannic acid procedure, which stabilizes extruded proteinaceous substances, was used to demonstrate exocytosis of DCV cores. Fresh brian slices containing the SCN were incubated in media containing high levels of potassium (56 mM) or glutamate (10 mM) in the presence of tannic acid. Long-term slice explant cultures of the SCN were similarly treated. Exocytosis from DCVs occurred from axonal terminals, from dendrites, and occasionally from somata. The sites of DCV exocytosis were generally nonsynaptic or para-synaptic, including release immediately adjacent to axo-spinous synaptic densities. These observations show that, in the suprachiasmatic nucleus, neuroactive substances contained in DCVs do not necessarily function at synaptic contract zones, but could also act as neuromodulators at non-synaptic sites.
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A major source of non-synaptically released OT and AVP is the magnocellular neurons in the hypothalamus, although there is also evidence that hypothalamic parvocellular neurons can release neuropeptides from dense core vesicles non-synaptically.
“A major source of non-synaptically released OT and AVP is the magnocellular neurons in the hypothalamus, although there is also evidence that hypothalamic parvocellular neurons can also release neuropeptides from dense core vesicles non-synaptically [111].”
From Cross-talk among oxytocin and arginine-vasopressin receptors: Relevance for basic and clinical studies of the brain and periphery (2018) · cites this paper as [111]
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- Authors
- Castel, M., Morris, J., & Belenky, M.
- Affiliations
- University of Oxford (GB)
- Hebrew University of Jerusalem (IL)
- ORCID
- Morris, John
- Venue
- NeuroReport
- Published
- 1996-01
- Volume
- 7
- Issue
- 2
- Pages
- 543-547
- ISSN
- 0959-4965 (ISSN-L)
- OpenAlex
- W1977561781
- Cited by
- 61
- References
- 0
- Citations by year
- 2024: 1 · 2023: 1 · 2022: 2 · 2020: 2 · 2019: 1 · 2018: 5 · 2017: 2 · 2016: 4 · 2015: 3 · 2014: 3 · 2013: 2 · 2012: 1
- FWCI
- 1.20
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Neuroreport (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Neuroscience › Endocrine and Autonomic Systems › Circadian rhythm and melatonin
- Keywords
- suprachiasmatic nucleus, dense core vesicles, exocytosis, dendritic release, axonal terminals, neuromodulation, tannic acid, ultrastructure, neurosecretion
- MeSH terms
- Animals, Dendrites, Exocytosis, Extracellular Space, Male, Microscopy, Electron, Suprachiasmatic Nucleus, Synapses, Presynaptic Terminals, Rats, In Vitro Techniques
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