Measuring Protein Synthesis With SUnSET: A Valid Alternative to Traditional Techniques?
Open accessAbstract
In Brief Protein synthesis rates commonly are measured using isotopic tracers to quantify the incorporation of a labeled amino acid into muscle proteins. Here we provide evidence supporting our hypothesis that the nonisotopic SUnSET technique is a valid and accurate method for the measurement of in vivo changes in protein synthesis at the whole-muscle and single–muscle fiber levels. SUnSET is a valid and accurate method for measuring protein synthesis at the whole-muscle and single–muscle fiber level.
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Protein synthesis was measured by surface sensing of translation (puromycin incorporation) as previously described.
“Protein synthesis was measured by surface sensing of translation, as previously described [12].”
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- Authors
- Goodman, C. A., & Hornberger, T. A.
- Affiliations
- University of Wisconsin–Madison (US)
- Corresponding author
- Goodman, Craig A.
- Venue
- Exercise and Sport Sciences Reviews
- Published
- 2013-04
- Volume
- 41
- Issue
- 2
- Pages
- 107-115
- ISSN
- 0091-6331 (ISSN-L)
- PMC
- PMC3951011
- OpenAlex
- W2072152524
- Cited by
- 233
- References
- 36
- Citations by year
- 2026: 9 · 2025: 18 · 2024: 18 · 2023: 28 · 2022: 29 · 2021: 32 · 2020: 17 · 2019: 21 · 2018: 19 · 2017: 17 · 2016: 14 · 2015: 7 · 2014: 3 · 2013: 1
- FWCI
- 0.32
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Exercise and Sport Sciences Reviews (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institute of Arthritis and Musculoskeletal and Skin Diseases (AR-057347, R56AR057347, R01AR057347)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Muscle metabolism and nutrition
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
- Keywords
- sunset, protein synthesis, muscle protein synthesis, single muscle fibers, method validation
- MeSH terms
- Animals, Humans, Muscle Proteins, Protein Synthesis Inhibitors, Puromycin, Muscle, Skeletal
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