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Cited by 39Fungal and yeast genetics researchCellular transport and secretionProtein Kinase Regulation and GTPase Signaling

A mutation in the Rho1-GAP-encoding gene BEM2 of Saccharomyces cerevisiae affects morphogenesis and cell wall functionality

Cid, Víctor J., Cenamor, Rosa, Sánchez, Miguel, Nombela, César·1998·Microbiology
DOI 10.1099/00221287-144-1-25
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Abstract

Summary: Saccharomyces cerevisiae strain V918 was previously isolated in a search for thermosensitive autolytic mutants and found to bear a recessive mutation that caused the development of multinucleate swollen cells undergoing cell lysis. The BEM2 gene has been isolated by complementation of the phenotype of a V918 segregant. BEM2 encodes a Rho-GTPase-activating protein (GAP) which is thought to act as a modulator of the Rho1 small GTPase. It is shown that the mutation causing the morphogenetic and autolytic phenotype in strain V918 and its segregants lies in the BEM2 gene, defining a new mutant allele, bem2-21. Mutants in the BEM2 gene have been reported to display loss of cell polarity and depolarization of the actin cytoskeleton, causing a bud-emergence defect. Low resistance to sonication and to hydrolytic enzymes proved that the cell wall is less protective in bem2-21 mutants than in wild-type strains. Moreover, bem2-21 mutants are more sensitive than the wild-type to several antifungal drugs. Transmission electron microscopy revealed the development of abnormally thick and wide septa and the existence of thin areas in the cell wall which probably account for cell lysis. The depolarization of actin in bem2-21 mutants did not preclude morphogenetic events such as cell elongation in homozygous diploid strains during nitrogen starvation in solid media, hyperpolarization of growth in a background bearing a mutated septin, or sporulation. Multinucleate cells from bem2-21 homozygous diploids underwent sporulation giving rise to multispored asci (‘polyads’), containing up to 36 spores. This phenomenon occurred only under osmotically stabilized conditions, suggesting that the integrity of the ascus wall is impaired in cells expressing the bem2-21 mutation. It is concluded that the function of the BEM2 gene product is essential for the maintenance of a functional cell wall.

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Crossref · 10.1099/00221287-144-1-25

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  • Bem2 in S. cerevisiae acts as a GAP for Rho1 and is implicated in antifungal sensitivity, actin cytoskeleton organization, and bud emergence.

    “Bem2 in S. cerevisiae acts as a GAP for Rho1 and is implicated in antifungal sensitivity (assumed to be due to increased permeability of the cell surface), actin cytoskeleton organization, and bud emergence (35,36).”

    From The Rho1 GTPase-activating protein CgBem2 is required for survival of azole stress in Candida glabrata (2011) · cites this paper as [35]

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Authors
Cid, V. J., Cenamor, R., Sánchez, M., & Nombela, C.
Affiliations
  • Departamento de Microbiología II, Facultad de Farmacia, Universidad Complutense de Madrid, 28040 Madrid, Spain
  • Universidad Complutense de Madrid (ES)
Corresponding author
Sánchez, Miguel
ORCID
Nombela, César
Venue
Microbiology
Publisher
Microbiology Society
Published
1998-01-01
Volume
144
Issue
1
Pages
25-36
ISSN
  • 1350-0872 (ISSN-L)
  • 1465-2080
DOI
10.1099/00221287-144-1-25
OpenAlex
W2152106868
Cited by
39
References
50
Citations by year
2025: 1 · 2022: 2 · 2021: 1 · 2017: 1 · 2016: 1 · 2014: 1 · 2013: 1 · 2012: 1
FWCI
1.40
Type
journal article
Language
en
Open access
closed
Locations
  • Microbiology (publishedVersion)
  • PubMed (publishedVersion)
Funders
Ministerio de Economía y Competitividad
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
Keywords
RhoGAP, Saccharomyces cerevisiae, cell morphogenesis, cell wall integrity, cell lysis, multinucleated cells, cell polarity, bud emergence, septa, antifungal susceptibility, polyads, sporulation, nitrogen starvation, osmotic stress
MeSH terms
Alleles, Antifungal Agents, Cell Wall, Fungal Proteins, Genetic Complementation Test, Microbial Sensitivity Tests, Microscopy, Electron, Morphogenesis, Mutation, Proteins, Saccharomyces cerevisiae, Protein Biosynthesis, GTPase-Activating Proteins, Saccharomyces cerevisiae Proteins

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