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Cited by 68Antifungal resistance and susceptibilityBacterial biofilms and quorum sensingFungal and yeast genetics research

Bacterial lipopolysaccharides variably modulate in vitro biofilm formation of Candida species

Bandara, H. M. H. N., Lam, O. L. T., Watt, R. M., Jin, L. J., Samaranayake, L. P.·2010·Journal of Medical Microbiology
DOI 10.1099/jmm.0.021832-0
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Abstract

The objective of this study was to evaluate the effect of the bacterial endotoxin LPS on Candida biofilm formation in vitro. The effect of the LPS of Pseudomonas aeruginosa, Klebsiella pneumoniae, Serratia marcescens and Salmonella typhimurium on six different species of Candida, comprising Candida albicans ATCC 90028, Candida glabrata ATCC 90030, Candida krusei ATCC 6258, Candida tropicalis ATCC 13803, Candida parapsilosis ATCC 22019 and Candida dubliniensis MYA 646, was studied using a standard biofilm assay. The metabolic activity of in vitro Candida biofilms treated with LPS at 90 min, 24 h and 48 h was quantified by XTT reduction assay. Viable biofilm-forming cells were qualitatively analysed using confocal laser scanning microscopy (CLSM), while scanning electron microscopy (SEM) was employed to visualize the biofilm structure. Initially, adhesion of C. albicans was significantly stimulated by Pseudomonas and Klebsiella LPS. A significant inhibition of Candida adhesion was noted for the following combinations: C. glabrata with Pseudomonas LPS, C. tropicalis with Serratia LPS, and C. glabrata, C. parapsilosis or C. dubliniensis with Salmonella LPS (P<0.05). After 24 h of incubation, a significant stimulation of initial colonization was noted for the following combinations: C. albicans/C. glabrata with Klebsiella LPS, C. glabrata/C. tropicalis/C. krusei with Salmonella LPS. In contrast, a significant inhibition of biofilm formation was observed in C. glabrata/C. dubliniensis/C. krusei with Pseudomonas LPS, C. krusei with Serratia LPS, C. dubliniensis with Klebsiella LPS and C. parapsilosis/C. dubliniensis /C. krusei with Salmonella LPS (P<0.05). On further incubation for 48 h, a significant enhancement of biofilm maturation was noted for the following combinations: C. glabrata/C. tropicalis with Serratia LPS, C. dubliniensis with Klebsiella LPS and C. glabrata with Salmonella LPS, and a significant retardation was noted for C. parapsilosis/C. dubliniensis/C. krusei with Pseudomonas LPS, C. tropicalis with Serratia LPS, C. glabrata/C. parapsilosis/C. dubliniensis with Klebsiella LPS and C. dubliniensis with Salmonella LPS (P<0.05). These findings were confirmed by SEM and CLSM analyses. In general, the inhibition of the biofilm development of LPS-treated Candida spp. was accompanied by a scanty architecture with a reduced numbers of cells compared with the profuse and densely colonized control biofilms. These data are indicative that bacterial LPSs modulate in vitro Candida biofilm formation in a species-specific and time-dependent manner. The clinical and the biological relevance of these findings have yet to be explored.

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  • Biofilms persist due to their physical presence and population density, affording an almost inducible resistant phenotype despite distinct genetic alterations.

    “It is known that planktonic cells generally rely on irreversible genetic changes to maintain a resistant phenotype, while biofilms are able to persist due to their physical presence and the density of the population, which affords an almost inducible resistant phenotype notwithstanding of distinct genetic alterations [130,157].”

    From Candida glabrata Biofilms: How Far Have We Come? (2017) · cites this paper as [157]

  • Planktonic cells generally rely on irreversible genetic changes to maintain a resistant phenotype.

    “It is known that planktonic cells generally rely on irreversible genetic changes to maintain a resistant phenotype, while biofilms are able to persist due to their physical presence and the density of the population, which affords an almost inducible resistant phenotype notwithstanding of distinct genetic alterations [130,157].”

    From Candida glabrata Biofilms: How Far Have We Come? (2017) · cites this paper as [157]

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Authors
Bandara, H. M. H. N., Lam, O. L. T., Watt, R. M., Jin, L. J., & Samaranayake, L. P.
Affiliations
  • Oral Biosciences, Faculty of Dentistry, University of Hong Kong, 5/F Prince Philip Dental Hospital, 34 Hospital Road, Sai Ying Pun, Hong Kong SAR
  • University of Hong Kong (HK)
  • Prince Philip Dental Hospital (CN)
ORCID
Venue
Journal of Medical Microbiology
Publisher
Microbiology Society
Published
2010-10-01
Volume
59
Issue
10
Pages
1225-1234
ISSN
  • 0022-2615 (ISSN-L)
  • 1473-5644
DOI
10.1099/jmm.0.021832-0
OpenAlex
W2134250526
Cited by
68
References
43
Citations by year
2025: 4 · 2024: 2 · 2023: 3 · 2021: 1 · 2020: 6 · 2019: 5 · 2018: 7 · 2017: 4 · 2016: 7 · 2015: 8 · 2014: 4 · 2013: 9 · 2012: 4
FWCI
3.28
Type
journal article
Language
en
Open access
closed
Locations
  • Journal of Medical Microbiology (publishedVersion)
  • PubMed (publishedVersion)
  • Bristol Research (University of Bristol) (publishedVersion)
  • The HKU Scholars Hub (University of Hong Kong) (submittedVersion)
  • Explore Bristol Research (submittedVersion)
Topics
  • Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
Keywords
lipopolysaccharide, bacterial endotoxin, Candida albicans, Candida glabrata, Candida tropicalis, Candida parapsilosis, Candida krusei, Candida dubliniensis, Pseudomonas aeruginosa, Klebsiella pneumoniae, Serratia marcescens, Salmonella typhimurium, yeast-bacteria interactions, biofilm adhesion, biofilm maturation, biofilm inhibition
MeSH terms
Antibiosis, Candida, Cell Adhesion, Cell Wall, Gram-Negative Bacteria, Humans, Lipopolysaccharides, Oxidation-Reduction, Tetrazolium Salts, Time Factors, Biofilms, Microscopy, Confocal, Microbial Viability

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