Highly adherent small-colony variants of Pseudomonas aeruginosa in cystic fibrosis lung infection
Abstract
Pseudomonas aeruginosa, an opportunistic human pathogen and ubiquitous environmental bacterium, is capable of forming specialized bacterial communities, referred to as biofilm. The results of this study demonstrate that the unique environment of the cystic fibrosis (CF) lung seems to select for a subgroup of autoaggregative and hyperpiliated P. aeruginosa small-colony variants (SCVs). These morphotypes showed increased fitness under stationary growth conditions in comparison with clonal wild-types and fast-growing revertants isolated from the SCV population in vitro. In accordance with the SCVs being hyperpiliated, they exhibited increased twitching motility and capacity for biofilm formation. In addition, the SCVs attached strongly to the pneumocytic cell line A549. The emergence of these highly adherent SCVs within the CF lung might play a key role in the pathogenesis of P. aeruginosa lung infection, where a biofilm mode of growth is thought to be responsible for persistent infection.
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Several SCV isolates were more hydrophobic than wild-type P. aeruginosa and were hyperpiliated with type IV pili, which presumably contribute to aggregative phenotype.
“Several SCV isolates were more hydrophobic than wild-type P. aeruginosa and were hyperpiliated with type IV pili, which presumably contribute to the aggregative phenotype [134].”
From Bacterial autoaggregation (2018) · cites this paper as [134]
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Self-citation
- Authors
- Häußler, S., Ziegler, I., Löttel, A., Götz, F. V., Rohde, M., Wehmhöhner, D., Saravanamuthu, S., Tümmler, B., & Steinmetz, I.
- Affiliations
- Medizinische Hochschule Hannover (DE)
- Venue
- Journal of Medical Microbiology
- Publisher
- Microbiology Society
- Published
- 2003-04-01
- Volume
- 52
- Issue
- 4
- Pages
- 295-301
- ISSN
- 0022-2615 (ISSN-L)
- 1473-5644
- OpenAlex
- W2156638962
- Cited by
- 228
- References
- 36
- Citations by year
- 2026: 5 · 2025: 6 · 2024: 3 · 2023: 7 · 2022: 9 · 2021: 14 · 2020: 5 · 2019: 8 · 2018: 10 · 2017: 10 · 2016: 7 · 2015: 14 · 2014: 21 · 2013: 12 · 2012: 13
- FWCI
- 3.57
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Medical Microbiology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Cystic Fibrosis Research Advances
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Infections and bacterial resistance
- Keywords
- Pseudomonas aeruginosa, small colony variants, cystic fibrosis lung infection, biofilm formation, twitching motility, self-aggregation, bacterial adhesion, A549 cells, persistent infection, pathogenesis
- MeSH terms
- Bacterial Adhesion, Cell Line, Cystic Fibrosis, Flow Cytometry, Humans, Lung, Movement, Fimbriae, Bacterial, Pseudomonas aeruginosa, Pseudomonas Infections, Biofilms, Hydrophobic and Hydrophilic Interactions
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