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PreprintCited by 1Microbial Metabolic Engineering and BioproductionBiofuel production and bioconversionMicrobial metabolism and enzyme function

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Absolute proteome quantification in the gas-fermenting acetogen Clostridium autoethanogenum

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Valgepea, Kaspar, Talbo, Gert, Takemori, Nobuaki, Takemori, Ayako, Ludwig, Christina, Mueller, Alexander P., Tappel, Ryan, Köpke, Michael, Simpson, Séan Dennis, Nielsen, Lars Keld, Marcellin, Esteban·2021·bioRxiv (Cold Spring Harbor Laboratory)
DOI 10.1101/2021.05.11.443690

Preprint — published as 10.1128/msystems.00026-22

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Abstract

Microbes that can recycle one-carbon (C1) greenhouse gases into fuels and chemicals are vital for the biosustainability of future industries. Acetogens are the most efficient known microbes for fixing carbon oxides CO 2 and CO. Understanding proteome allocation is important for metabolic engineering as it dictates metabolic fitness. Here, we use absolute proteomics to quantify intracellular concentrations for >1,000 proteins in the model-acetogen Clostridium autoethanogenum grown on three gas mixtures. We detect prioritisation of proteome allocation for C1 fixation and significant expression of proteins involved in the production of acetate and ethanol as well as proteins with unclear functions. The data also revealed which isoenzymes are important. Integration of proteomic and metabolic flux data demonstrated that enzymes catalyse high fluxes with high concentrations and high in vivo catalytic rates. We show that flux adjustments were dominantly accompanied with changing enzyme catalytic rates rather than concentrations. Our work serves as a reference dataset and advances systems-level understanding and engineering of acetogens.

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Metadata

Authors
Valgepea, K., Talbo, G., Takemori, N., Takemori, A., Ludwig, C., Mueller, A. P., Tappel, R., Köpke, M., Simpson, S. D., Nielsen, L. K., & Marcellin, E.
Affiliations
  • The University of Queensland (AU)
  • Ehime University (JP)
  • Technical University of Munich (DE)
Corresponding author
Marcellin, Esteban
ORCID
Venue
bioRxiv (Cold Spring Harbor Laboratory)
Publisher
openRxiv
Published
2021-05-12
DOI
10.1101/2021.05.11.443690
OpenAlex
W3162214286
Cited by
1
References
6
Citations by year
2022: 1
Type
posted content
Language
en
Open access
Locations
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion)
  • Technical University of Denmark, DTU Orbit (Technical University of Denmark, DTU) (submittedVersion, cc-by)
Funders
  • Australian Government
  • European Commission
  • Australian Research Council (ARC LP140100213, LP140100213)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
  • Physical Sciences › Engineering › Biomedical Engineering › Biofuel production and bioconversion
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial metabolism and enzyme function
Keywords
Clostridium autoethanogenum, acetogenic bacteria, CO2 fixation, proteome allocation, isozymes, metabolic flux, acetate production, ethanol production, metabolic engineering

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