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PreprintCited by 21Genomics and Chromatin DynamicsMicrotubule and mitosis dynamicsNuclear Structure and Function

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Mapping the invisible chromatin transactions of prophase chromosome remodelling

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Samejima, Itaru, Spanos, Christos, Samejima, Kumiko, Rappsilber, Juri, Kustatscher, Georg, Earnshaw, William C.·2021·Molecular Cell
DOI 10.1101/2021.06.21.449273PMC PMC8823707

Preprint — published as 10.1016/j.molcel.2021.12.039 · Paper page

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Abstract

SUMMARY: We have used a combination of chemical genetics, chromatin proteomics and imaging to map the earliest chromatin transactions during vertebrate cell entry into mitosis. Chicken DT40 CDK1 as cells undergo synchronous mitotic entry within 15 minutes following release from a 1NM-PP1-induced arrest in late G 2 . In addition to changes in chromatin association with nuclear pores and the nuclear envelope, earliest prophase is dominated by changes in the association of ribonucleoproteins with chromatin, particularly in the nucleolus, where pre-rRNA processing factors leave chromatin significantly before RNA polymerase I. Nuclear envelope barrier function is lost early in prophase and cytoplasmic proteins begin to accumulate on the chromatin. As a result, outer kinetochore assembly appears complete by nuclear envelope breakdown (NEBD). Most interphase chromatin proteins remain associated with chromatin until NEBD, after which their levels drop sharply. An interactive proteomic map of chromatin transactions during mitotic entry is available as a resource at https://mitoChEP.bio.ed.ac.uk .

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Metadata

Authors
Samejima, I., Spanos, C., Samejima, K., Rappsilber, J., Kustatscher, G., & Earnshaw, W. C.
Affiliations
  • Wellcome Centre for Cell Biology (GB)
  • University of Edinburgh (GB)
Corresponding author
  • Kustatscher, Georg
  • Earnshaw, William C.
ORCID
Venue
Molecular Cell
Publisher
openRxiv
Published
2021-06-21
Volume
82
Issue
3
Pages
696-708.e4
ISSN
1097-2765 (ISSN-L)
DOI
10.1101/2021.06.21.449273
PMC
PMC8823707
OpenAlex
W4210330911
Cited by
21
References
1
Citations by year
2026: 2 · 2025: 6 · 2024: 7 · 2023: 4 · 2022: 2
FWCI
1.54
Type
journal article
Language
en
Open access
Licence
cc-by
Locations
  • Molecular Cell (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • Edinburgh Research Explorer (University of Edinburgh) (publishedVersion, cc-by)
  • Edinburgh Research Explorer (University of Edinburgh) (submittedVersion, cc-by)
Funders
  • Wellcome Trust (221044, 107022, 107022/Z/15/Z, 203149)
  • Medical Research Council (203149, MR/T03050X/1)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
Keywords
mitotic entry, CDK1, nuclear envelope breakdown, ribonucleoprotein, nucleolus, pre-rRNA processing, nuclear pore complex, chromatin proteomics, DT40 cells, G2 cell cycle arrest
MeSH terms
Animals, Chickens, Chromatin, Chromosomes, DNA, Nuclear Proteins, Prophase, Protein Binding, RNA, Ribosomal, Time Factors, CDC2 Protein Kinase, Lymphoma, B-Cell, Proteome, Lamin Type B, Proteomics, Chromatin Assembly and Disassembly, Cell Line, Tumor

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