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PreprintCited by 15Algal biology and biofuel productionMicrobial Metabolic Engineering and BioproductionLipid metabolism and biosynthesis

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Proteomics unveil a central role for peroxisomes in butyrate assimilation of the heterotrophic Chlorophyte alga Polytomella sp

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Lacroux, J., Atteia, A., Brugière, S., Couté, Y., Vallon, O., Steyer, J.-P., van Lis, R.·2022·Frontiers in Microbiology
DOI 10.1101/2022.08.29.505702PMC PMC9637915

Preprint — published as 10.3389/fmicb.2022.1029828

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Abstract

Volatile fatty acids found in effluents of the dark fermentation of biowastes can be used for mixotrophic growth of microalgae, improving productivity and reducing the cost of the feedstock. Microalgae can use the acetate in the effluents very well, but butyrate is poorly assimilated and can inhibit growth above 1 gC.L -1 . The non-photosynthetic chlorophyte alga Polytomella sp. SAG 198.80 was found to be able to assimilate butyrate fast. To decipher the metabolic pathways implicated in butyrate assimilation, quantitative proteomics study was developed comparing Polytomella sp. cells grown on acetate and butyrate at 1 gC.L -1 . After statistical analysis, a total of 1772 proteins were retained, of which 119 proteins were found to be overaccumulated on butyrate vs. only 46 on acetate, indicating that butyrate assimilation necessitates additional metabolic steps. The data show that butyrate assimilation occurs in the peroxisome via the β-oxidation pathway to produce acetyl-CoA and further tri/dicarboxylic acids in the glyoxylate cycle. Concomitantly, reactive oxygen species defense enzymes as well as the branched amino acid degradation pathway were strongly induced.Although no clear dedicated butyrate transport mechanism could be inferred, several membrane transporters induced on butyrate are identified as potential condidates. Metabolic responses correspond globally to the increased needs for central cofactors NAD, ATP and CoA, especially in the peroxisome and the cytosol.

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Metadata

Authors
Lacroux, J., Atteia, A., Brugière, S., Couté, Y., Vallon, O., Steyer, J.-P., & van Lis, R.
Affiliations
  • Université de Montpellier (FR)
  • Centre National de la Recherche Scientifique (FR)
Corresponding author
van Lis, R.
ORCID
Venue
Frontiers in Microbiology
Publisher
openRxiv
Published
2022-08-29
Volume
13
Pages
1029828-1029828
ISSN
1664-302X (ISSN-L)
DOI
10.1101/2022.08.29.505702
PMC
PMC9637915
OpenAlex
W4307265348
Cited by
15
References
1
Citations by year
2026: 6 · 2025: 6 · 2024: 2 · 2023: 1
FWCI
0.41
Type
journal article
Language
en
Open access
Licence
cc-by
Locations
  • Frontiers in Microbiology (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • HAL (submittedVersion)
  • HAL (submittedVersion, cc-by)
  • Institutional Archive of Ifremer (French Research Institute for Exploitation of the Sea) (submittedVersion, other-oa)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • European Commission (ANR-17-EURE-0003)
  • Agence Nationale de la Recherche (ANR-10-INBS-0008, ANR-10-INBS-08, ANR-17-EURE-0003, ANR-17-EURE-, 10-INBS-08, LABX-0011-01, Proteomics French Infrastructure,ANR-10-INBS-08, ANR-17, ANR-10, ANR-11-LABX-0011)
  • Centre National de la Recherche Scientifique (ANR-11-LABX-0011-01, ANR-11-LABX-0011, 11-LABX-0011-01, ANR‐17‐EURE‐0003)
  • Institut Carnot Santé Animale
  • Région Occitanie Pyrénées-Méditerranée
  • Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
Topics
  • Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Keywords
peroxisomes, Polytomella, Chlorophyta, dark fermentation effluent, volatile fatty acids, fatty acid beta-oxidation, glyoxylate cycle, acetyl-CoA, branched-chain amino acid degradation, reactive oxygen species defense, membrane transporters, acetate, proteomics

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