Acetate Utilization and Butyryl Coenzyme A (CoA):Acetate-CoA Transferase in Butyrate-Producing Bacteria from the Human Large Intestine

Duncan, Sylvia H.; Barcenilla, Adela; Stewart, Colin S.; Pryde, Susan E.; Flint, Harry J.

doi:10.1128/aem.68.10.5186-5190.2002

Cited by 743Gut microbiota and healthMicrobial Fuel Cells and BioremediationAnaerobic Digestion and Biogas Production

Acetate Utilization and Butyryl Coenzyme A (CoA):Acetate-CoA Transferase in Butyrate-Producing Bacteria from the Human Large Intestine

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Duncan, Sylvia H., Barcenilla, Adela, Stewart, Colin S., Pryde, Susan E., Flint, Harry J.·2002·Applied and Environmental Microbiology

DOI 10.1128/aem.68.10.5186-5190.2002PMC PMC126392

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Abstract

Seven strains of Roseburia sp., Faecalibacterium prausnitzii , and Coprococcus sp. from the human gut that produce high levels of butyric acid in vitro were studied with respect to key butyrate pathway enzymes and fermentation patterns. Strains of Roseburia sp. and F. prausnitzii possessed butyryl coenzyme A (CoA):acetate-CoA transferase and acetate kinase activities, but butyrate kinase activity was not detectable either in growing or in stationary-phase cultures. Although unable to use acetate as a sole source of energy, these strains showed net utilization of acetate during growth on glucose. In contrast, Coprococcus sp. strain L2-50 is a net producer of acetate and possessed detectable butyrate kinase, acetate kinase, and butyryl-CoA:acetate-CoA transferase activities. These results demonstrate that different functionally distinct groups of butyrate-producing bacteria are present in the human large intestine.

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Members of Lachnospiraceae are potent producers of SCFAs.
“The members of Lachnospiraceae are potent producers of SCFAs (Duncan et al., 2002).”
From The Microbiota-Gut-Brain Axis (2019) · cites this paper as Duncan et al., 2002
The “Butyrate-producing isolates” section reports that the seven human fecal strains studied produced at least 10 mM butyrate in vitro, including Roseburia and Coprococcus strains. The paper does not identify them as Lachnospiraceae or establish that members of the family generally are potent producers of SCFAs; its evidence is for selected strains and chiefly for butyrate. (LLM, conf=0.86)
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Pathways of acetate, propionate, and butyrate formation by the human fecal microbial flora
Miller, T. L., & Wolin, M. J. (1996) · Applied and Environmental Microbiology · doi:10.1128/aem.62.5.1589-1592.1996 · Cited by 481 · see citing works

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[11]11. Duncan, S. H., G. L. Hold, H. J. M. Harmsen, C. S. Stewart, and H. J. Flint. Growth requirements and fermentation products of Fusobacterium prausnitzii , and a proposal to reclassify it as Faecalibacterium gen. nov., comb. nov. Int. J. Syst. Evol. Microbiol., in press.
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“31 Moreover, their relative abundances are increased in obese rodent models, linked to metabolic disorder 32–34 and colon cancer.”

Authors: Duncan, S. H., Barcenilla, A., Stewart, C. S., Pryde, S. E., & Flint, H. J.
Affiliations: University of Aberdeen (GB)
Corresponding author: Duncan, Sylvia H.
ORCID: Duncan, Sylvia H.
Flint, Harry J.
Venue: Applied and Environmental Microbiology
Publisher: American Society for Microbiology
Published: 2002-10
Volume: 68
Issue: 10
Pages: 5186-5190
ISSN: 0099-2240 (ISSN-L)
1098-5336
DOI: 10.1128/aem.68.10.5186-5190.2002
PMC: PMC126392
OpenAlex: W2166569595
Cited by: 743
References: 1
Citations by year: 2026: 41 · 2025: 58 · 2024: 64 · 2023: 81 · 2022: 54 · 2021: 91 · 2020: 65 · 2019: 49 · 2018: 40 · 2017: 39 · 2016: 32 · 2015: 16 · 2014: 15 · 2013: 19 · 2012: 10
FWCI: 1.74
Type: journal article
Language: en
Open access: green
www.ncbi.nlm.nih.gov
Licence: journals.asm.org/non-commercial-tdm-license (tdm)
Locations: Applied and Environmental Microbiology (publishedVersion)
Europe PMC (PubMed Central) (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
Physical Sciences › Environmental Science › Environmental Engineering › Microbial Fuel Cells and Bioremediation
Physical Sciences › Engineering › Building and Construction › Anaerobic Digestion and Biogas Production
Keywords: acetate utilization, butyrate production, acetate kinase, butyrate kinase, Roseburia, Faecalibacterium prausnitzii, Coprococcus, human gut microbiome, butyrate-producing bacteria, bacterial functional groups, glucose fermentation
MeSH terms: Acetates, Bacteria, Butyrates, Culture Media, Fermentation, Humans, Intestine, Large, Coenzyme A-Transferases

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Acetate Utilization and Butyryl Coenzyme A (CoA):Acetate-CoA Transferase in Butyrate-Producing Bacteria from the Human Large Intestine

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