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PreprintCited by 29T-cell and B-cell ImmunologyAdvanced Proteomics Techniques and ApplicationsGenomics and Chromatin Dynamics

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An integrated proteome and transcriptome of B cell maturation defines poised activation states of transitional and mature B cells

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Salerno, Fiamma, Howden, Andrew J. M., Matheson, Louise S., Gizlenci, Özge, Screen, Michael, Lingel, Holger, Brunner-Weinzierl, Monika C., Turner, Martin·2022·Nature Communications
DOI 10.1101/2022.12.22.521580PMC PMC10447577

Preprint — published as 10.1038/s41467-023-40621-2 · Paper page

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Abstract

During B cell maturation, transitional and mature B cells acquire cell-intrinsic features that determine their ability to exit quiescence and mount effective immune responses. We used label-free mass spectrometry to quantify the proteome of B cell subsets from the mouse spleen and map the differential expression of environmental sensing, transcription- and translation initiation-factors that define cellular identity and function. By comparing the full-length transcriptome and proteome within the same sample, we identified mRNAs linked to B cell activation and antibody secretion that are expressed without detectable protein. These “poised” mRNAs might enable rapid protein production through increased translation or protein stability. In addition, through interrogation of our proteomic dataset, we found that the translational repressor PDCD4 restrains the response of marginal zone B cells to a T-independent antigen. Our molecular characterization of B cell maturation is a valuable resource to further explore the mechanisms underpinning the specialised functions of B cell subsets.

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Metadata

Authors
Salerno, F., Howden, A. J. M., Matheson, L. S., Gizlenci, Ö., Screen, M., Lingel, H., Brunner-Weinzierl, M. C., & Turner, M.
Affiliations
  • Babraham Institute (GB)
  • University of Dundee (GB)
  • Otto-von-Guericke-Universität Magdeburg (DE)
Corresponding author
  • Salerno, Fiamma
  • Turner, Martin
ORCID
Venue
Nature Communications
Publisher
openRxiv
Published
2022-12-22
Volume
14
Issue
1
Pages
5116-5116
ISSN
2041-1723 (ISSN-L)
DOI
10.1101/2022.12.22.521580
PMC
PMC10447577
OpenAlex
W4386095572
Cited by
29
References
1
Citations by year
2026: 11 · 2025: 9 · 2024: 7 · 2023: 2
FWCI
3.16
Type
journal article
Language
en
Open access
Licence
cc-by
Locations
  • Nature Communications (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • Discovery Research Portal (University of Dundee) (submittedVersion, other-oa)
  • RePEc: Research Papers in Economics (submittedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • University of Dundee
  • Wellcome Trust (200823/Z/16/Z)
  • Cancer Research UK
  • European Commission (841930)
  • Deutsche Forschungsgemeinschaft (Br1860/12, SFB-854, SFB854 B14)
  • Directorate for Biological Sciences
  • Horizon 2020 Framework Programme (765158)
  • Biotechnology and Biological Sciences Research Council (BBS/E/B/000C0428, BBS/E/B/000C0409, BBS/E/B/000C0407, BBS/E/B/000C0427)
Topics
  • Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
  • Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
Keywords
B cell maturation, transitional B cells, marginal zone B cells, proteome, transcriptome, PDCD4, protein synthesis inhibition, B cell activation, immunoglobulin secretion, T-independent antigens, environmental sensing, translation initiation factors, spleen
MeSH terms
Animals, B-Lymphocytes, Cell Differentiation, RNA, Messenger, Transcription Factors, Protein Biosynthesis, B-Lymphocyte Subsets, RNA-Binding Proteins, Proteome, Apoptosis Regulatory Proteins, Mice, Transcriptome

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