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PreprintCited by 46Microtubule and mitosis dynamicsAdvanced Electron Microscopy Techniques and ApplicationsSperm and Testicular Function

The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.

In-cell structural insight into the stability of sperm microtubule doublet

Open access
Tai, Linhua, Yin, Guoliang, Huang, Xiaojun, Zhu, Yun, Sun, Fei·2023·Cell Discovery
DOI 10.1101/2023.05.30.542832PMC PMC10663601

Preprint — published as 10.1038/s41421-023-00606-3

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Abstract

The propulsion for mammalian sperm swimming is generated by flagella beating. Microtubule doublets (DMTs) along with microtubule inner proteins (MIPs) are essential structural blocks of flagella. However, the intricate molecular architecture of sperm DMT remains elusive. Here, by in situ cryo-electron tomography, we solved the in-cell structure of mouse sperm DMT up to 3.6 Å resolution and built its model with 36 kinds of MIPs in 48 nm periodicity. We identified multiple copies of Tektin5 that reinforce Tektin bundle, and multiple MIPs with different periodicities that anchor the Tektin bundle to tubulin wall. This architecture contributes to a superior stability of A-tubule than B-tubule of DMT, which was revealed by structural comparison of DMTs from the intact and deformed axonemes. Our work provides an overall molecular picture of sperm DMT that is essential to understand the molecular mechanism of sperm motility as well as the related ciliopathies.

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Metadata

Authors
Tai, L., Yin, G., Huang, X., Zhu, Y., & Sun, F.
Affiliations
Chinese Academy of Sciences (CN)
Corresponding author
  • Zhu, Yun
  • Sun, Fei
ORCID
Venue
Cell Discovery
Publisher
openRxiv
Published
2023-05-31
Volume
9
Issue
1
Pages
116-116
ISSN
2056-5968 (ISSN-L)
DOI
10.1101/2023.05.30.542832
PMC
PMC10663601
OpenAlex
W4388866037
Cited by
46
References
1
Citations by year
2026: 11 · 2025: 20 · 2024: 15
FWCI
6.63
Type
journal article
Language
en
Open access
Licence
cc-by
Locations
  • Cell Discovery (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • National Natural Science Foundation of China (31925026, 32071187, 31830020, 61932018)
  • Chinese Academy of Sciences (31830020)
  • Medical Research Council
  • National Key Research and Development Program of China (2021YFA1301500, 31830020, 2019YFA0904101)
  • Institute of Biophysics, Chinese Academy of Sciences
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Structural Biology › Advanced Electron Microscopy Techniques and Applications
  • Health Sciences › Medicine › Reproductive Medicine › Sperm and Testicular Function
Keywords
flagella, sperm motility, microtubule inner proteins, axoneme structure, cryo-electron tomography, ciliopathies, mouse sperm

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