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PreprintCited by 10Microtubule and mitosis dynamicsGenomics and Chromatin DynamicsDNA Repair Mechanisms

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Molecular basis for condensin enrichment at pericentromeres

Open access
Wang, Menglu, Zou, Juan, Spanos, Christos, Rappsilber, Juri, Marston, Adele L.·2024·The EMBO Journal
DOI 10.1101/2024.03.27.586992PMC PMC11791182

Preprint — published as 10.1038/s44318-024-00336-6

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Abstract

Summary: Faithful chromosome segregation requires packaging of the genome on both global and local scales. Condensin plays a crucial role at pericentromeres to resist spindle forces and ensure the bioriented attachment of kinetochores to microtubules in mitosis. Here we demonstrate that budding yeast condensin is recruited to pericentromeres through a direct interaction between its Ycg1 subunit and the pericentromeric adaptor protein, shugoshin (Sgo1). We identify a Short Linear Motif (SLiM), termed CR1, within the C-terminal region of Sgo1 which inserts into a conserved pocket on Ycg1. Disruption of this interface abolishes the Sgo1-condensin interaction, prevents condensin recruitment to pericentromeres and results in defective sister kinetochore biorientation in mitosis. Similar motifs to CR1 are found in known and potential condensin binding partners and the Ycg1 binding pocket is broadly conserved, including in the mammalian homolog CAP-G. Overall, we uncover the molecular mechanism that targets condensin to define a specialized chromosomal domain.

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Metadata

Authors
Wang, M., Zou, J., Spanos, C., Rappsilber, J., & Marston, A. L.
Affiliations
Wellcome Centre for Cell Biology (GB)
ORCID
Venue
The EMBO Journal
Publisher
openRxiv
Published
2024-03-28
Volume
44
Issue
3
Pages
705-735
ISSN
0261-4189 (ISSN-L)
DOI
10.1101/2024.03.27.586992
PMC
PMC11791182
OpenAlex
W4405468320
Cited by
10
References
1
Citations by year
2026: 3 · 2025: 5 · 2024: 1
FWCI
2.02
Type
journal article
Language
en
Open access
Licence
cc-by
Locations
  • The EMBO Journal (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
  • Edinburgh Research Explorer (University of Edinburgh) (submittedVersion, cc-by)
Funders
Wellcome Trust (226791, 108504, 220780, 2183052, 203149)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
Keywords
condensin, SMC complex, chromosome condensation, Sgo1, pericentromeres, ribosomal DNA, kinetochore tension, chromosome segregation, mitosis, Saccharomyces cerevisiae, chromatin looping
MeSH terms
Adenosine Triphosphatases, Chromosomal Proteins, Non-Histone, DNA-Binding Proteins, DNA, Ribosomal, Mitosis, Nuclear Proteins, Saccharomyces cerevisiae, Chromosomes, Fungal, Kinetochores, Cell Cycle Proteins, Chromosome Segregation, Saccharomyces cerevisiae Proteins, Multiprotein Complexes

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