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PreprintCited by 24Genomics and Chromatin DynamicsMicrotubule and mitosis dynamicsNuclear Structure and Function

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Condensin II activation by M18BP1

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Borsellini, Alessandro, Conti, Duccio, Cutts, Erin, Harris, Rebecca J., Walstein, Kai, Graziadei, Andrea, Cecatiello, Valentina, Aarts, Tom F., Xie, Ren, Mazouzi, Abdelghani, Sen, Sushweta, Hoencamp, Claire, Pleuger, Richard, Ghetti, Sabrina, Oberste-Lehn, Lina, Pan, Dongqing, Bange, Tanja, Haarhuis, Judith H.I., Perrakis, Anastassis, Brummelkamp, Thijn R., Rowland, Benjamin D., Musacchio, Andrea, Vannini, Alessandro·2024·Molecular Cell
DOI 10.1101/2024.05.02.592151

Preprint — published as 10.1016/j.molcel.2025.06.014

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Abstract

Condensin complexes promote the drastic spatial rearrangement of the genome upon mitotic entry. Condensin II initiates chromosome condensation in early mitosis. To prevent chromosome condensation during interphase, condensin II is inhibited by MCPH1, but the mechanism is unknown. Through genetic and proteomic approaches, we identify M18BP1, a protein previously associated with centromere identity, as a factor required for condensin II localization to chromatin. M18BP1 directly binds condensin II’s CAP-G2 subunit and competes with MCPH1 for binding. Upon mitotic entry, CDK1 mediated phosphorylation may promote a switch from MCPH1 to M18BP1 binding to activate condensin II. Our results identify a fundamental and evolutionarily conserved mechanism of condensin II activation.

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  1. 10.1038/s41580-023-00609-8 · DOI
  2. 10.1242/jcs.01604 · DOI
  3. 10.1091/mbc.E04-03-0242 · DOI
  4. 10.1126/science.aam9702 · DOI
  5. 10.1016/S0092-8674(03)00724-4 · DOI
  6. 10.1242/jcs.097790 · DOI
  7. 10.1101/gad.2060311 · DOI
  8. 10.1126/science.aao6135 · DOI
  9. 10.1083/jcb.201801048 · DOI
  10. 10.1016/j.cell.2009.02.039 · DOI
  11. 10.1083/jcb.200708170 · DOI
  12. 10.1016/j.celrep.2015.11.041 · DOI
  13. 10.1038/nature10179 · DOI
  14. 10.1074/mcp.M116.057885 · DOI
  15. 10.1038/ncomms5588 · DOI
  16. 10.1083/jcb.201005136 · DOI
  17. 10.1371/journal.pone.0006831 · DOI
  18. 10.1091/mbc.E15-12-0843 · DOI
  19. 10.1016/S1534-5807(03)00199-0 · DOI
  20. 10.1091/mbc.E08-11-1127 · DOI
  21. 10.7554/eLife.73348 · DOI
  22. 10.3389/fcell.2022.914249 · DOI
  23. 10.1007/s00018-012-1048-8 · DOI
  24. 10.1016/j.devcel.2006.11.002 · DOI
  25. 10.1083/jcb.200701066 · DOI
  26. 10.1016/j.cell.2009.02.040 · DOI
  27. 10.15252/embr.201643564 · DOI · Paper page
  28. 10.1038/s41467-019 · DOI
  29. 10.7554/eLife.23352 · DOI
  30. 10.1016/j.cell.2014.06.016 · DOI
  31. 10.1016/j.molcel.2016.11.021 · DOI
  32. 10.1016/j.celrep.2016.05.004 · DOI
  33. 10.1074/jbc.M113.529958 · DOI
  34. 10.1101/2024.02.23.581696 · DOI
  35. 10.1101/2024.02.23.581399 · DOI · Paper page
  36. 10.12688/wellcomeopenres.16482.1 · DOI
  37. Evans, R. , O’Neill, M. , Pritzel, A. , Antropova, N. , A, S., Green, T. , Žídek, A., Bates, R., Blackwell, S., Yim, J., et al. (2021). Protein complex prediction with AlphaFold- Multimer. bioRxiv. · DOI · Paper page
  38. 10.1038/s41594-020-0457-x · DOI
  39. 10.1016/j.devcel.2017.06.021 · DOI
  40. 10.7554/eLife.78984 · DOI
  41. 10.1016/j.molcel.2019.09.020 · DOI
  42. 10.1016/j.molcel.2020.04.026 · DOI
  43. 10.1016/S0959-440X(03)00037-X · DOI
  44. 10.1006/jmbi.2000.4379 · DOI
  45. 10.1016/S0092-8674(00)80890-9 · DOI
  46. 10.1016/j.molcel.2019.03.037 · DOI
  47. 10.1126/science.aan6516 · DOI
  48. 10.15252/embj.201797596 · DOI
  49. 10.1016/j.molcel.2016.02.014 · DOI
  50. 10.1002/jcp.28681 · DOI
  51. 10.7554/eLife.89812 · DOI
  52. 10.1126/sciadv.1700191 · DOI
  53. 10.1016/j.molcel.2015.07.007 · DOI
  54. 10.1101/gad.1675908 · DOI
  55. 10.1101/gad.1675708 · DOI
  56. 10.15252/embj.201592849 · DOI
  57. 10.1038/nsmb.1708 · DOI
  58. 10.7554/eLife.75475 · DOI
  59. 10.7554/eLife.01374 · DOI
  60. 10.1371/journal.pgen.1008569 · DOI
  61. 10.1016/j.molcel.2020.04.026 · DOI
  62. 10.1073/pnas.2120006119 · DOI · Paper page
  63. 10.1126/science.abm4012 · DOI · Paper page
  64. 10.1091/mbc.E23-10-0392 · DOI
  65. 10.1242/jcs.259088 · DOI
  66. 10.1038/s41594-023-00968-y · DOI
  67. 10.1038/s41586-019-1910-z · DOI · Paper page
  68. 10.1101/2024.03.27.586992 · DOI · Paper page
  69. 10.1101/2024.03.19.585759 · DOI
  70. 10.1242/jcs.01556 · DOI
  71. 10.1083/jcb.200712028 · DOI
  72. 10.1016/j.xpro.2021.100407 · DOI
  73. 10.1038/nprot.2006.417 · DOI
  74. 10.1016/bs.mie.2023.02.007 · DOI
  75. 10.1074/mcp.M111.011015 · DOI · Paper page
  76. 10.1038/nbt.1511 · DOI · Paper page
  77. 10.1038/nprot.2016.136 · DOI
  78. 10.1126/science.aac7557 · DOI · Paper page
  79. 10.1038/nmeth.2019 · DOI · Paper page
  80. Otsu, N . (1979). A Threshold Selection Method from Gray-Level Histograms. IEEE TRANSACTIONS ON SYSTEMS, MAN, AND CYBERNETICS 9. · DOI
  81. 10.1083/jcb.202001064 · DOI · Paper page
  82. 10.1038/nprot.2006.468 · DOI
  83. 10.1021/acs.analchem.6b04935 · DOI
  84. 10.15252/msb.20198994 · DOI
  85. 10.1021/acs.analchem.7b04915 · DOI
  86. 10.1021/bi048233g · DOI
  87. 10.1038/nmeth.4193 · DOI
  88. 10.1016/j.jsb.2015.08.008 · DOI
  89. 10.7554/eLife.42166 · DOI
  90. 10.1042/BCJ20210708 · DOI
  91. 10.1038/s41592-019-0575-8 · DOI
  92. 10.1038/s41592-020-01049-4 · DOI
  93. 10.1093/nar/gkw408 · DOI · Paper page
  94. 10.1002/pro.3943 · DOI
  95. 10.1093/nar/gkab1038 · DOI · Paper page

Metadata

Authors
Borsellini, A., Conti, D., Cutts, E., Harris, R. J., Walstein, K., Graziadei, A., Cecatiello, V., Aarts, T. F., Xie, R., Mazouzi, A., Sen, S., Hoencamp, C., Pleuger, R., Ghetti, S., Oberste-Lehn, L., Pan, D., Bange, T., Haarhuis, J. H. I., Perrakis, A., Brummelkamp, T. R., Rowland, B. D., Musacchio, A., & Vannini, A.
Affiliations
  • Human Technopole (IT)
  • Max Planck Institute of Molecular Physiology (DE)
  • University of Sheffield (GB)
  • The Netherlands Cancer Institute (NL)
Corresponding author
  • Cutts, Erin
  • Vannini, Alessandro
ORCID
Venue
Molecular Cell
Publisher
openRxiv
Published
2024-05-03
Volume
85
Issue
14
Pages
2688-2700.e11
ISSN
1097-2765 (ISSN-L)
DOI
10.1101/2024.05.02.592151
OpenAlex
W4412002214
Cited by
24
References
1
Citations by year
2026: 15 · 2025: 5 · 2024: 4
FWCI
5.98
Type
journal article
Language
en
Open access
Licence
cc-by
Locations
  • Molecular Cell (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
  • White Rose Research Online (University of Leeds, The University of Sheffield, University of York) (acceptedVersion)
  • Universitätsbibliographie, Universität Duisburg-Essen (submittedVersion)
Funders
  • European Molecular Biology Organization (ALTF 552-2022, ALTF 439-2019)
  • Wellcome Trust (200818/Z/16/Z)
  • Cancer Research UK
  • Deutsche Forschungsgemeinschaft
  • Nederlandse Organisatie voor Wetenschappelijk Onderzoek (VI.C.202.098)
  • Max-Planck-Gesellschaft
  • KWF Kankerbestrijding (12687, 15868)
  • HORIZON EUROPE European Research Council (951430)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
Keywords
condensin II, MCPH1, chromosome condensation, mitosis, centromere identity, condensin I, protein-protein interaction
MeSH terms
Adenosine Triphosphatases, Carrier Proteins, Chromatin, DNA-Binding Proteins, HeLa Cells, Humans, Interphase, Mitosis, Protein Binding, Cell Cycle Proteins, Multiprotein Complexes

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