CoChat
Sign Up
PreprintCited by 14Protein Structure and DynamicsRNA Research and SplicingMicrotubule and mitosis dynamics

The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.

Cellular Function of a Biomolecular Condensate Is Determined by Its Ultrastructure

Open access
Scholl, Daniel, Boyd, Tumara, Latham, Andrew P., Salazar, Alexandra, Khan, Asma, Boeynaems, Steven, Holehouse, Alex S., Lander, Gabriel C., Sali, Andrej, Park, Donghyun, Deniz, Ashok A., Lasker, Keren·2024·Nature Structural & Molecular Biology
DOI 10.1101/2024.12.27.630454PMC PMC11703246

Preprint — published as 10.1038/s41594-025-01742-y · Paper page

Open this paper in CoChat — free account

Abstract

Biomolecular condensates play key roles in the spatiotemporal regulation of cellular processes. Yet, the relationship between atomic features and condensate function remains poorly understood. We studied this relationship using the polar organizing protein Z (PopZ) as a model system, revealing how its material properties and cellular function depend on its ultrastructure. We revealed PopZ’s hierarchical assembly into a filamentous condensate by integrating cryo-electron tomography, biochemistry, single-molecule techniques, and molecular dynamics simulations. The helical domain drives filamentation and condensation, while the disordered domain inhibits them. Phase-dependent conformational changes prevent interfilament contacts in the dilute phase and expose client binding sites in the dense phase. These findings establish a multiscale framework that links molecular interactions and condensate ultrastructure to macroscopic material properties that drive cellular function.

Overview

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1101/2024.12.27.630454

Cites

1 of 1 references verified0 citation errors · 0 not checkable

Statistics

Verification

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Citations

Cites

Flagged first, then by citations

Research works printed but not linked: 100

  1. 10.1038/nrm.2017.7 · DOI
  2. 10.1126/science.aaf4382 · DOI
  3. 10.1016/j.jmb.2023.167971 · DOI
  4. 10.1038/s41580-020-00303-z · DOI · Paper page
  5. 10.1146/annurev-biophys-121219-081629 · DOI
  6. 10.1038/s41580-024-00789-x · DOI
  7. 10.1126/science.aad9964 · DOI
  8. 10.1038/s41580-020-00326-6 · DOI
  9. 10.1038/s41570-024-00635-w · DOI
  10. 10.1021/acs.chemrev.2c00814 · DOI
  11. 10.1016/j.molcel.2022.05.018 · DOI
  12. 10.1038/s41586-022-05138-6 · DOI
  13. 10.1038/s41567-024-02558-1 · DOI
  14. 10.1016/j.cell.2016.04.047 · DOI
  15. 10.1021/jacs.4c08946 · DOI
  16. 10.1016/j.sbi.2019.09.007 · DOI
  17. 10.1016/j.cell.2020.07.037 · DOI
  18. 10.1038/nature08908 · DOI
  19. 10.1111/febs.15672 · DOI
  20. 10.1016/j.molcel.2018.08.027 · DOI
  21. 10.1073/pnas.1616133114 · DOI
  22. 10.1016/j.cell.2008.07.015 · DOI
  23. 10.1016/j.cell.2008.07.016 · DOI
  24. 10.1111/j.1365-2958.2010.07088.x · DOI
  25. 10.1098/rsbl.2008.0793 · DOI
  26. 10.1016/j.mib.2012.10.011 · DOI
  27. 10.1016/j.cub.2020.07.033 · DOI
  28. 10.1016/j.mib.2016.06.007 · DOI
  29. 10.1038/s41564-019-0647-7 · DOI
  30. 10.1038/s41467-022-33221-z · DOI
  31. 10.3791/64435 · DOI
  32. 10.1016/j.jmb.2021.167423 · DOI
  33. 10.1016/S0022-2836(62)80112-0 · DOI
  34. 10.1074/jbc.M009033200 · DOI
  35. 10.1111/mmi.12398 · DOI
  36. 10.1083/jcb.201303036 · DOI
  37. 10.1073/pnas.1602380113 · DOI
  38. 10.1073/pnas.1405188111 · DOI
  39. 10.1002/anie.202001578 · DOI
  40. 10.1126/science.aar5839 · DOI
  41. 10.1016/j.cbpa.2006.08.017 · DOI
  42. 10.1038/s41586-020-2992-3 · DOI
  43. 10.1016/bs.mie.2020.06.009 · DOI
  44. 10.1016/j.jmb.2018.08.003 · DOI
  45. 10.1016/j.jmb.2020.09.020 · DOI
  46. 10.1038/nature05321 · DOI
  47. 10.1038/s41586-024-07487-w · DOI · Paper page
  48. 10.1016/j.jmb.2021.167348 · DOI
  49. 10.1021/acs.chemrev.2c00586 · DOI
  50. 10.1016/j.sbi.2021.08.006 · DOI
  51. 10.1002/pro.4094 · DOI
  52. 10.1371/journal.pcbi.1011442 · DOI
  53. 10.1371/journal.pcbi.1005941 · DOI
  54. 10.1371/journal.pcbi.1004794 · DOI
  55. 10.1002/1873-3468.14554 · DOI
  56. 10.7554/eLife.60416 · DOI
  57. 10.1073/pnas.0404236101 · DOI
  58. 10.1038/s41592-023-02159-5 · DOI
  59. 10.1016/j.sbi.2015.03.008 · DOI
  60. 2006, Separating structural heterogeneities from stochastic variations in fluorescence resonance energy transfer distributions via photon distribution analysis, J Phys Chem B, 110, 6970, 10.1021/jp057257+ · DOI
  61. 10.1128/JB.01239-07 · DOI
  62. 10.1038/ncb2083 · DOI
  63. 10.1073/pnas.1005274107 · DOI
  64. 10.1093/nar/gkad982 · DOI
  65. Flory, P.J. (1953). Principles of polymer chemistry (Cornell university press).
  66. Rubinstein, M. , and Colby, R.H. (2003). Polymer physics (Oxford university press). · DOI · Paper page
  67. 10.1016/0001-8686(87)85003-0 · DOI
  68. 10.1016/j.isci.2022.104105 · DOI
  69. 10.1073/pnas.2220014120 · DOI
  70. 10.1038/nature10879 · DOI
  71. 10.1038/nature22386 · DOI
  72. 10.1126/science.aar7432 · DOI
  73. 10.1021/acs.biochem.8b00058 · DOI
  74. 10.7554/eLife.31486 · DOI
  75. 10.1016/j.cell.2018.06.006 · DOI
  76. 10.1126/science.aaw8653 · DOI · Paper page
  77. 10.1016/j.cell.2016.05.004 · DOI
  78. 10.1016/j.devcel.2020.09.003 · DOI
  79. 10.1016/j.tibs.2014.08.006 · DOI
  80. 10.1016/j.celrep.2015.03.062 · DOI
  81. 10.1038/s41467-020-14343-8 · DOI
  82. 10.15252/embj.201798308 · DOI
  83. 10.1038/s41422-018-0017-7 · DOI
  84. 10.1146/annurev.biochem.75.103004.142647 · DOI
  85. 10.1073/pnas.74.5.2021 · DOI
  86. 10.1038/281583a0 · DOI
  87. 10.1016/S0021-9258(19)68818-6 · DOI
  88. 10.1146/annurev-cellbio-032320-094706 · DOI · Paper page
  89. 10.1111/febs.15056 · DOI
  90. 2020, SPOP and cancer: a systematic review, Am J Cancer Res, 10, 704
  91. 10.1038/S41585-020-0314-Z · DOI
  92. 10.15252/embj.201593169 · DOI
  93. 10.1016/j.molcel.2022.12.033 · DOI
  94. 10.7554/eLife.84147 · DOI · Paper page
  95. 10.1073/pnas.0912008106 · DOI
  96. 10.1016/j.jmb.2015.10.002 · DOI
  97. 10.1074/jbc.M600204200 · DOI
  98. 10.1021/jacs.9b10066 · DOI
  99. 10.1016/j.molcel.2023.05.025 · DOI
  100. 10.1073/pnas.1504822112 · DOI · Paper page

Metadata

Authors
Scholl, D., Boyd, T., Latham, A. P., Salazar, A., Khan, A., Boeynaems, S., Holehouse, A. S., Lander, G. C., Sali, A., Park, D., Deniz, A. A., & Lasker, K.
Affiliations
  • Scripps Research Institute (US)
  • University of California, San Francisco (US)
  • Baylor College of Medicine (US)
  • Washington University in St. Louis (US)
Corresponding author
  • Park, Donghyun
  • Deniz, Ashok A.
  • Lasker, Keren
ORCID
Venue
Nature Structural & Molecular Biology
Publisher
openRxiv
Published
2024-12-28
Volume
33
Issue
3
Pages
420-432
ISSN
1545-9985 (ISSN-L)
DOI
10.1101/2024.12.27.630454
PMC
PMC11703246
OpenAlex
W7127082149
Cited by
14
References
1
Citations by year
2026: 12 · 2025: 2
FWCI
24.63
Type
journal article
Language
en
Open access
Licence
Locations
  • Nature Structural & Molecular Biology (publishedVersion, cc-by-nc-nd)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
  • PubMed Central (publishedVersion, cc-by-nc-nd)
  • PubMed Central (submittedVersion, cc-by-nc-nd)
  • Digital Commons-TMC (Texas Medical Center) (submittedVersion)
Funders
  • National Institutes of Health (1s10od032467-01)
  • National Institute of General Medical Sciences (R01GM083960, F32GM150243, R35GM130375)
  • National Institute of Neurological Disorders and Stroke (DP2NS142714, R01NS095892)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Structure and Dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
Keywords
PopZ, biomolecular condensates, helical domain, intrinsically disordered regions, phase separation, cell growth

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review