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PreprintCited by 7Plant Parasitism and ResistancePlant-Microbe Interactions and ImmunityPlant biochemistry and biosynthesis

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Convergent strigolactone perception via independent horizontal gene transfer of pre-adapted α/β-hydrolases

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Wang, Qia, Ye, Ye, Wang, Lulu, Guan, Yanlong, Wang, Shuanghua, Wang, Zhe, Sun, Hang, Smith, Steven M., Huang, Jinling·2025·Molecular Plant
DOI 10.1101/2025.03.14.642314

Preprint — published as 10.1016/j.molp.2025.09.021

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Abstract

Strigolactones (SLs) are not only phytohormones that influence multiple aspects of plant growth and development, but also signaling molecules for interactions between plants and certain fungi or bacteria. In plants, the SL receptor is an α/β-hydrolase (ABH) encoded by the D14 / KAI2 gene family, which is known to be derived from proteobacterial RsbQ through horizontal gene transfer (HGT). Here we show that the CpD14 - like ( CDL ) gene family, which encodes another ABH exhibiting SL receptor functions in fungi, was acquired from actinobacteria via an independent HGT event. X-ray crystallographic experiments reveal that CDL and D14/KAI2/RsbQ (DKR) proteins have distinctive lid structures but maintain the same core ‘α/β fold’ and catalytic triad. Biochemical assays further show that both actinobacterial CDL and proteobacterial RsbQ can recognize and hydrolyze SLs, suggesting that they are pre-adapted for SL perception. This work demonstrates that independent HGT of pre-adapted genes can trigger convergent evolution of key innovations and provides insights into how SLs are sensed by organisms across kingdoms.

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Metadata

Authors
Wang, Q., Ye, Y., Wang, L., Guan, Y., Wang, S., Wang, Z., Sun, H., Smith, S. M., & Huang, J.
Affiliations
  • Kunming Institute of Botany (CN)
  • Biortus (China) (CN)
  • China Pharmaceutical University (CN)
  • University of Tasmania (AU)
Corresponding author
  • Smith, Steven M.
  • Huang, Jinling
ORCID
Venue
Molecular Plant
Publisher
openRxiv
Published
2025-03-17
Volume
18
Issue
11
Pages
1949-1961
ISSN
1674-2052 (ISSN-L)
DOI
10.1101/2025.03.14.642314
OpenAlex
W4414695375
Cited by
7
References
1
Citations by year
2026: 4 · 2025: 3
FWCI
3.78
Type
journal article
Language
en
Open access
Licence
Locations
  • Molecular Plant (publishedVersion)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
Funders
  • National Natural Science Foundation of China (32170242, 32470244)
  • Ministry of Science and Technology of the People's Republic of China (2019QZKK0502)
  • Youth Innovation Promotion Association of the Chinese Academy of Sciences (2022398)
  • Yunnan Provincial Science and Technology Department (202201AT070163)
  • People's Government of Yunnan Province (XDYC-QNRC-2022-0046)
  • National Key Research and Development Program of China (2024YFF1306700)
  • Australian Research Council Centre of Excellence for Plant Success in Nature and Agriculture (CE 200100015)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Parasitism and Resistance
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant-Microbe Interactions and Immunity
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant biochemistry and biosynthesis
Keywords
strigolactones, horizontal gene transfer, alpha/beta hydrolase, strigolactone perception, DWARF14, Cryphonectria parasitica, actinobacteria, Proteobacteria, plant-fungal interactions, protein evolution, lid domain, ligand specificity
MeSH terms
Ascomycota, Bacteria, Fungi, Heterocyclic Compounds, 3-Ring, Hydrolases, Hydrolysis, Lactones, Phylogeny, Gene Transfer, Horizontal

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