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Highly multiplexed in situ protein imaging with signal amplification by Immuno-SABER
Open accessPreprint — published as 10.1038/s41587-019-0207-y
Abstract
Probing the molecular organization of tissues requires in situ analysis by microscopy. However current limitations in multiplexing, sensitivity, and throughput collectively constitute a major barrier for comprehensive single-cell profiling of proteins. Here, we report Immunostaining with Signal Amplification By Exchange Reaction (Immuno-SABER), a rapid, highly multiplexed signal amplification method that simultaneously tackles these key challenges. Immuno-SABER utilizes DNA-barcoded antibodies and provides a method for highly multiplexed signal amplification via modular orthogonal DNA concatemers generated by Primer Exchange Reaction. This approach offers the capability to preprogram and control the amplification level independently for multiple targets without in situ enzymatic reactions, and the intrinsic scalability to rapidly amplify and image a large number of protein targets. We validated our approach in diverse sample types including cultured cells, cryosections, FFPE sections, and whole mount tissues. We demonstrated independently tunable 5-180-fold amplification for multiple targets, covering the full signal range conventionally achieved by secondary antibodies to tyramide signal amplification, as well as simultaneous signal amplification for 10 different proteins using standard equipment and workflow. We further combined Immuno-SABER with Expansion Microscopy to enable rapid and highly multiplexed super-resolution tissue imaging. Overall, Immuno-SABER presents an effective and accessible platform for rapid, multiplexed imaging of proteins across scales with high sensitivity.
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Deep Residual Learning for Image Recognition
He, K., Zhang, X., Ren, S., & Sun, J. (2016) · 2016 IEEE Conference on Computer Vision and Pattern Recognition (CVPR) · doi:10.1109/cvpr.2016.90 · Cited by 235,474
U-Net: Convolutional Networks for Biomedical Image Segmentation
Ronneberger, O., Fischer, P., & Brox, T. (2015) · Lecture notes in computer science · doi:10.1007/978-3-319-24574-4_28 · Cited by 99,404
Fiji: an open-source platform for biological-image analysis
Schindelin, J., Arganda-Carreras, I., Frise, E., Kaynig, V., Longair, M., Pietzsch, T., Preibisch, S., Rueden, C., Saalfeld, S., Schmid, B., et al. (2012) · Nature Methods · doi:10.1038/nmeth.2019 · Cited by 66,890
Tissue-based map of the human proteome
Uhlén, M., Fagerberg, L., Hallström, B. M., Lindskog, C., Oksvold, P., Mardinoglu, A., Sivertsson, Å., Kampf, C., Sjöstedt, E., Asplund, A., et al. (2015) · Science · doi:10.1126/science.1260419 · Cited by 15,286
Global quantification of mammalian gene expression control
Schwanhäusser, B., Busse, D., Li, N., Dittmar, G., Schuchhardt, J., Wolf, J., Chen, W., & Selbach, M. (2011) · Nature · doi:10.1038/nature10098 · Cited by 6,566
CellProfiler: image analysis software for identifying and quantifying cell phenotypes
Carpenter, A. E., Jones, T. R., Lamprecht, M. R., Clarke, C., Kang, I. H., Friman, O., Guertin, D. A., Chang, J. H., Lindquist, R. A., Moffat, J., et al. (2006) · Genome biology · doi:10.1186/gb-2006-7-10-r100 · Cited by 5,212
Simultaneous epitope and transcriptome measurement in single cells
Stoeckius, M., Hafemeister, C., Stephenson, W., Houck-Loomis, B., Chattopadhyay, P. K., Swerdlow, H., Satija, R., & Smibert, P. (2017) · Nature Methods · doi:10.1038/nmeth.4380 · Cited by 3,098
Spatially resolved, highly multiplexed RNA profiling in single cells
Chen, K. H., Boettiger, A. N., Moffitt, J. R., Wang, S., & Zhuang, X. (2015) · Science · doi:10.1126/science.aaa6090 · Cited by 2,969
(2012) · doi:10.1016/j.jmoldx.2011.08.002 · Cited by 2,567
Regev, A., Teichmann, S. A., Lander, E. S., Amit, I., Benoist, C., Birney, E., Bodenmiller, B., Campbell, P., Carninci, P., Clatworthy, M., et al. (2017) · eLife · doi:10.7554/elife.27041 · Cited by 2,232
Flagged first, then by citations
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Items 401–487 of 487
Dissecting human organ development using spatial technologies
Zhang, X., Vandana, J. J., Greenspun, B. C., & Chen, S. (2026) · Developmental Cell · doi:10.1016/j.devcel.2026.05.004 · Cited by 1
Yang, L. T., & Yang, Z. (2026) · Journal of Translational Medicine · doi:10.1186/s12967-026-08577-1 · Cited by 1
Decoding Cardiovascular Disease Through Spatial Proteomics
Ma, M., Lian, Y., Shang, E., Xue, C., Wei, M., Zhang, M., Sundararaman, N., Parker, S. J., Eyk, J. E. V., & Qu, J. (2026) · Circulation Research · doi:10.1161/circresaha.126.327475 · Cited by 2
PASTA: versatile tyramide-oligonucleotide amplification for multimodal spatial biology
Michel, H. A., McCallum, P. A., Wu, W., Qiu, H., Lee, J. L., Luo, S., Chan, C. N., Schaffenrath, J., Yeo, Y. Y., Yiu, S. P. T., et al. (2026) · Nature Methods · doi:10.1038/s41592-026-03200-z · Cited by 1
Self-citationLi, X., Xie, Y., Liu, W., Niu, M., Wang, H., & Zhang, Y. (2026) · Sensors and Actuators B Chemical · doi:10.1016/j.snb.2026.140751 · Cited by 1
Li, C., Rashid, R., Sadhu, E. M., Santagata, S., & Becich, M. J. (2023) · Health informatics · doi:10.1007/978-3-031-27173-1_5 · Cited by 1
The use of tissue clearing to study renal transport mechanisms and kidney remodelling
Saritas, T. (2023) · Current Opinion in Nephrology & Hypertension · doi:10.1097/mnh.0000000000000904 · Cited by 1
CRISPR/Cas-based electrochemical diagnostics
Rad, M. B. (2023) · Royal Society of Chemistry eBooks · doi:10.1039/bk9781839169366-00372 · Cited by 1
Lv, Y., Fu, Q., Xu, Y., & Shi, P. (2023) · Small · doi:10.1002/smll.202306638 · Cited by 1
Diao, N., Hou, J., Peng, X., Wang, Y., He, A., Gao, H., Yang, L., Guo, P., Wang, J., & Han, D. (2024) · Angewandte Chemie · doi:10.1002/ange.202406330 · Cited by 1
Self-citationYao, L., Zhang, L., Chen, L., Fei, Y., Lamon, S., Gu, M., Mi, L., Wang, J., & Ma, J. (2024) · Journal of Photochemistry and Photobiology B Biology · doi:10.1016/j.jphotobiol.2024.113034 · Cited by 1
Tadakuma, H., & Kitagawa, D. (2020) · Biophysical Reviews · doi:10.1007/s12551-020-00646-z · Cited by 1
Indexable signal amplification for multiparametric imagingPreprint
Simonson, P. D., Valencia, I., & Patel, S. S. (2021) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2021.06.03.446843 · Cited by 1
IBEX: An open and extensible method for high content multiplex imaging of diverse tissuesPreprint
Radtke, A. J., Chu, C. J., Yaniv, Z., Li, Y., Marr, J., Beuschel, R. T., Ichise, H., Gola, A., Kabát, J., Lowekamp, B., et al. (2021) · arXiv (Cornell University) · doi:10.48550/arxiv.2107.11364 · Cited by 1
Pandit, K., Petrescu, J., Cuevas, M., Stephenson, W., Smibert, P., Phatnani, H. P., & Maniatis, S. (2021) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2021.08.27.457864 · Cited by 1
Catalytic DNA Polymerization Can Be Expedited by Active Product Release**
Moerman, P. G., Gavrilov, M. M., Ha, T., & Schulman, R. (2022) · Angewandte Chemie · doi:10.1002/ange.202114581 · Cited by 1
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Zhao, Y., Cheng, Z., Stefani, C., Skillman, T., Klimas, A., Lee, A., DiBernardo, E. F., Brown, K. M., Milman, T., Gallagher, B. R., et al. (2023) · Research Square · doi:10.21203/rs.3.rs-2637060/v1 · Cited by 1
Choi, S., Shin, S., Kim, Y.-J., Chung, H., Cho, H., & Yun, H. G. (2023) · Research Square · doi:10.21203/rs.3.rs-3301570/v1 · Cited by 1
Andrusaite, A. T., Ridgewell, O. J., Ahlback, A., Webster, H. C., Yamaguchi, H., Peel, M., Frede, A., Al-Khalidi, S., Farthing, A., Heawood, A. L. L., et al. (2024) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2024.09.11.612410 · Cited by 1
Taylor, A. J. (2024) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2024.10.01.615855 · Cited by 1
Zhong, S., Wang, R., Gao, X., Guo, Q., Lin, R., & Luo, M. (2025) · eLife · doi:10.7554/elife.105225 · Cited by 1
Spatial Proofreading Amplification of in situ Transcript and Protein SignalsPreprint
Tischbirek, C. H., Colón, K. L., Lobbia, S., Cronin, C. J., & Cai, L. (2025) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2025.06.11.659176 · Cited by 1
Zhong, S., Wang, R., Gao, X., Guo, Q., Lin, R., & Luo, M. (2025) · eLife · doi:10.7554/elife.105225.2 · Cited by 1
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Rajagopalan, A. R., Venkatesh, I., Aslam, R., Kirchenbüechler, D., Khanna, S., Cimbaluk, D. J., & Gupta, V. (2020) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2020.11.16.385237
Cereceda, K., Jorquera, R., & Villarroel‐Espíndola, F. (2021) · Advances in Laboratory Medicine / Avances en Medicina de Laboratorio · doi:10.1515/almed-2021-0051
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Faculty Opinions recommendation of Light microscopy of proteins in their ultrastructural context.
von Blume, J. (2021) · Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature · doi:10.3410/f.738410814.793583276
Nucleic Acid Conjugates for Biosensing – Design, Preparation, and Application
Ihara, T., Kitamura, Y., & Katsuda, Y. (2022) · Springer eBooks · doi:10.1007/978-981-16-1313-5_58-1
Nucleic Acid Conjugates for Biosensing: Design, Preparation, and Application
Ihara, T., Kitamura, Y., & Katsuda, Y. (2023) · Springer eBooks · doi:10.1007/978-981-19-9776-1_58
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Tomimatsu, K., Fujii, T., Bise, R., Hosoda, K., Taniguchi, Y., Ochiai, H., Ohishi, H., Ando, K., Minami, R., Tanaka, K. Y., et al. (2023) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2023.10.17.561810
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Seiffert, M., Cid, L. L., Wong, J., Floerchinger, A., Paul, Y., Schifflers, C., Mallm, J., Lichter, P., Iskar, M., Zapatka, M., et al. (2024) · Research Square · doi:10.21203/rs.3.rs-3909204/v1
Ma, J., Yao, L., Zhang, L., Chen, L., Fei, Y., Lamon, S., Gu, M., Mi, L., & Wang, J. (2024) · SSRN Electronic Journal · doi:10.2139/ssrn.4870458
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Yin, S., Wu, S., Li, Z., Lu, H., Wang, Z., Yan, Z., & Jin, X. (2024) · Advanced imaging. · doi:10.3788/ai.2024.10011
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Zhu, Z., Zhu, X., Deng, J., Yuan, Q., Chang, P., Gu, Z., & Shen, D. (2024) · Sensors and Actuators B Chemical · doi:10.1016/j.snb.2024.137113
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Akopyan, K., Hao, Z., Karlberg, S., Vakkilainen, S., Taylan, F., Pekkinen, M., Mäkitie, O., & Lindqvist, A. (2026) · EMBO Reports · doi:10.1038/s44319-026-00859-5
Wang, C., Zheng, J., Xiong, Z., Yin, W., Zhao, Y. D., Wu, H., Liang, S., Zhang, L., Yao, C., Deng, Z., et al. (2025) · Angewandte Chemie International Edition · doi:10.1002/anie.202511629
Wang, C., Zheng, J., Xiong, Z., Yin, W., Zhao, Y. D., Wu, H., Liang, S., Zhang, L., Yao, C., Deng, Z., et al. (2025) · Angewandte Chemie · doi:10.1002/ange.202511629
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Tanevski, J., Flores, R. O. R., Gábor, A., Schapiro, D., & Sáez-Rodríguez, J. (2022) · Figshare · doi:10.6084/m9.figshare.19603461
Xiao, Y., Luo, S., Qiu, J., Zhang, Y., Liu, W., Zhao, Y., Zhu, Y., Deng, Y., Lu, M., Liu, S., et al. (2024) · Figshare · doi:10.6084/m9.figshare.25294625
Tanevski, J., Flores, R. O. R., Gábor, A., Schapiro, D., & Sáez-Rodríguez, J. (2022) · Figshare · doi:10.6084/m9.figshare.19603464
Lang, G. (2025) · Springer eBooks · doi:10.1007/978-3-662-71093-7_11
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Sun, Y., Liu, R., Mu, J., Zhang, L., & Wang, Y. (2026) · British Journal of Pharmacology · doi:10.1111/bph.70487
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Kim, J., Choi, J. H., Kim, W., Cho, N., Jung, Y., Park, S., Choi, E., Lee, C., Kim, Y., Ryu, T., et al. (2026) · Nature Communications · doi:10.1038/s41467-026-74890-4 · Cited by 1
wang, H., Yang, X., Wang, S., Yang, Z., Yang, X., Yang, Y., Li, Z., Ren, Y., Zhang, Q., Zhao, B., et al. (2026) · iMeta · doi:10.1002/imt2.70146
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Westerhausen, M. T., Bergin, R. J., Phillips, C. R., Violi, J. P., Lockwood, T. E., Ridlen, R. M., & Bishop, D. (2026) · ACS Central Science · doi:10.1021/acscentsci.6c00422
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Lei, X., Cui, X., Ni, Y., Ding, Y., Miao, Y., Qian, C., & Shan, J. (2026) · Journal of Advanced Research · doi:10.1016/j.jare.2026.08.054
Meyer, L., Haefliger, S., Schulz, D., Fischer, J. R., & Bodenmiller, B. (2026) · Nature Reviews Methods Primers · doi:10.1038/s43586-026-00518-y
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Zhao, Y. (2026) · Elsevier eBooks · doi:10.1016/b978-0-323-99507-8.00087-1
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Metadata
- Authors
- Saka, S. K., Wang, Y., Kishi, J. Y., Zhu, A., Zeng, Y., Xie, W., Kirli, K., Yapp, C., Cicconet, M., Beliveau, B. J., Lapan, S. W., Yin, S., Lin, M., Boyden, E. S., Kaeser, P. S., Pihan, G., Church, G. M., & Yin, P.
- Affiliations
- Harvard University (US)
- MIT Media Lab (US)
- Beth Israel Deaconess Medical Center (US)
- Corresponding author
- Saka, Sinem K.
- Wang, Yu
- Yin, Peng
- ORCID
- Venue
- Nature Biotechnology
- Publisher
- openRxiv
- Published
- 2018-12-28
- Volume
- 37
- Issue
- 9
- Pages
- 1080-1090
- ISSN
- 0733-222X (ISSN-L)
- PMC
- PMC6728175
- OpenAlex
- W2968292465
- Cited by
- 485
- References
- 87
- Citations by year
- 2026: 58 · 2025: 74 · 2024: 81 · 2023: 61 · 2022: 87 · 2021: 68 · 2020: 48 · 2019: 7 · 2018: 1
- FWCI
- 17.37
- Type
- journal article
- Language
- en
- Open access
- green
- doi.org
- Licence
- cc-by-nc-nd
- Locations
- Nature Biotechnology (publishedVersion)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Digital Access to Scholarship at Harvard (DASH) (Harvard University) (acceptedVersion)
- DSpace@MIT (Massachusetts Institute of Technology) (submittedVersion, cc-by-nc-sa)
- Funders
- National Science Foundation (1317291)
- U.S. Department of Defense (N000141612410, N000141812549, N000141612182)
- Howard Hughes Medical Institute
- U.S. Department of Health and Human Services (R01NS083898, 1UG3HL145600/HuBMAP, 1U01MH106011, 1R01GM124401, R01MH113279, RM1 HG008525, 1R01EB018659, 1DP1GM133052, 5K99EY028215-02, 1R01MH113349)
- Damon Runyon Cancer Research Foundation
- Harvard Medical School
- National Institutes of Health (5k99ey028215-02, R01NS083898, RM1 HG008525, R01MH113279, NS072030, 1UG3HL145600/HuBMAP, 1UG3HL145600/HuBMAP, 1R01EB018659, 1U01MH106011, 1DP1GM133052, 1R01GM124401)
- Hansjörg Wyss Institute for Biologically Inspired Engineering, Harvard University
- National Heart, Lung, and Blood Institute (UG3HL145600)
- Office of Naval Research (N00014-16-1-2410, N00014-16-1-2182, N00014-18-1-2549, N00014-16-1-2410, (N00014-18-1-2549, N00014-16-1-2182, N00014)
- National Institute of Mental Health (U01MH106011, R01MH113349, R01MH113279)
- National Human Genome Research Institute (RM1HG008525)
- National Eye Institute (K99 EY028215)
- National Institute of General Medical Sciences (DP1GM133052, R01GM124401)
- National Institute of Neurological Disorders and Stroke (#NS072030, P30NS072030, R01NS083898)
- National Institute of Biomedical Imaging and Bioengineering (R01EB018659)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced biosensing and bioanalysis techniques
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Advanced Fluorescence Microscopy Techniques
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Molecular Biology Techniques and Applications
- Keywords
- primer exchange reaction, expansion microscopy, formalin-fixed paraffin-embedded sections, frozen section
- MeSH terms
- Animals, Antibodies, Cell Line, DNA, Fluorescent Dyes, Humans, Immunohistochemistry, Microscopy, Fluorescence, Proteins, Retina, Staining and Labeling, In Situ Hybridization, Fluorescence, Mice, DNA Barcoding, Taxonomic
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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