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POT1-interacting protein PIP1: a telomere length regulator that recruits POT1 to the TIN2/TRF1 complex
Open accessAbstract
Human telomere length is controlled by a negative feedback loop based on the binding of TRF1 to double-stranded telomeric DNA. The TRF1 complex recruits POT1, a single-stranded telomeric DNA-binding protein necessary for cis-inhibition of telomerase. By mass spectrometry, we have identified a new telomeric protein, which we have named POT1-interacting protein 1 (PIP1). PIP1 bound both POT1 and the TRF1-interacting factor TIN2 and could tether POT1 to the TRF1 complex. Reduction of PIP1 or POT1 levels with shRNAs led to telomere elongation, indicating that PIP1 contributes to telomere length control through recruitment of POT1.
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“TRF1-interacting nuclear factor 2 also interacts with the subcomplex of shelterin that binds the single-stranded overhang-TPP1 and POT1, two oligonucleotide/ oligosaccharide binding (OB)-fold containing proteins (13)(14)(15).”
From Telomeres and telomerase in cancer (2010) · cites this paper as [14]
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Metadata
- Authors
- Ye, J. Z.-S., Hockemeyer, D., Krutchinsky, A. N., Loayza, D., Hooper, S. M., Chait, B. T., & de Lange, T.
- Affiliations
- Memorial Sloan Kettering Cancer Center (US)
- Venue
- Genes & Development
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 2004-07-01
- Volume
- 18
- Issue
- 14
- Pages
- 1649-1654
- ISSN
- 0890-9369 (ISSN-L)
- 1549-5477
- PMC
- PMC478187
- OpenAlex
- W2155983703
- Cited by
- 459
- References
- 21
- Citations by year
- 2026: 4 · 2025: 7 · 2024: 15 · 2023: 18 · 2022: 6 · 2021: 21 · 2020: 12 · 2019: 15 · 2018: 15 · 2017: 16 · 2016: 30 · 2015: 21 · 2014: 34 · 2013: 17 · 2012: 24
- Type
- posted content
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Genes & Development (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (RR00862)
- National Cancer Institute (K08 CA93604, K08CA093604, T32 CA09673-26A1, T32CA009673)
- National Center for Research Resources (RR00862, P41RR000862)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Telomeres, Telomerase, and Senescence
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Aging › Genetics, Aging, and Longevity in Model Organisms
- Keywords
- PIP1, POT1, TIN2, TRF1, telomere length regulation, telomeric proteins, telomerase inhibition, telomere elongation, protein-protein interaction, telomeric DNA
- MeSH terms
- Shelterin Complex, Carrier Proteins, Cloning, Molecular, Fluorescent Antibody Technique, HeLa Cells, Humans, Mass Spectrometry, Telomere, Two-Hybrid System Techniques, Telomere-Binding Proteins, RNA Interference, Telomeric Repeat Binding Protein 1, Intracellular Signaling Peptides and Proteins
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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