The DOI matches its Crossref record; 40 of the 40 references it cites were found in a registry or an index.
Reinforcement of silencing at transposons and highly repeated sequences requires the concerted action of two distinct RNA polymerases IV in Arabidopsis
Open accessAbstract
Recent genetic and biochemical studies have revealed the existence in plants of a fourth RNA polymerase, RNAPIV, which mediates siRNA accumulation and DNA methylation-dependent silencing of endogenous repeated sequences. Here, we show that Arabidopsis expresses, in fact, two evolutionarily related forms of RNAPIV, hereafter referred to as RNAPIVa and RNAPIVb. These two forms contain the same second-largest subunit (NRPD2), but differ at least by their largest subunit, termed NRPD1a and NRPD1b. Unlike NRPD1a, NRPD1b possesses a reiterated CTD, a feature that also characterizes the largest subunit of RNAPII. Our data indicate that RNAPIVb is the most abundant form of RNAPIV in Arabidopsis. Selective disruption of either form of RNAPIV indicates that RNAPIVa-dependent siRNA accumulation is not sufficient per se to drive robust silencing at endogenous loci and that high levels of DNA methylation and silencing depend on siRNA that are accumulated through a pathway involving the concerted action of both RNAPIV forms. Taken together, our results imply the existence of a novel two-step mechanism in siRNA synthesis at highly methylated loci, with RNAPIVb being an essential component of a self-reinforcing loop coupling de novo DNA methylation to siRNA production.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Gapped BLAST and PSI-BLAST: a new generation of protein database search programs
Altschul, S. (1997) · Nucleic Acids Research · doi:10.1093/nar/25.17.3389 · Cited by 73,302
Analysis of the genome sequence of the flowering plant Arabidopsis thaliana
Initiative, T. A. G. (2000) · Nature · doi:10.1038/35048692 · Cited by 9,301
Genome-Wide Insertional Mutagenesis of Arabidopsis thaliana
Alonso, J. M., Stepanova, A. N., Leisse, T. J., Kim, C. J., Chen, H., Shinn, P., Stevenson, D. K., Zimmerman, J., Barajas, P., Cheuk, R., et al. (2003) · Science · doi:10.1126/science.1086391 · Cited by 5,102
Senescent cells, tumor suppression, and organismal aging: good citizens, bad neighbors
Campisi, J. (2005) · Cell · doi:10.1016/j.cell.2005.02.003 · Cited by 2,379
Genetic and Functional Diversification of Small RNA Pathways in Plants
Xie, Z., Johansen, L. K., Gustafson, A. M., Kasschau, K. D., Lellis, A. D., Zilberman, D., Jacobsen, S. E., & Carrington, J. C. (2004) · PLoS Biology · doi:10.1371/journal.pbio.0020104 · Cited by 1,584
Structural Basis of Transcription: RNA Polymerase II at 2.8 Ångstrom Resolution
Cramer, P., Bushnell, D. A., & Kornberg, R. D. (2001) · Science · doi:10.1126/science.1059493 · Cited by 1,247
Role of transposable elements in heterochromatin and epigenetic control
Lippman, Z., Gendrel, A.-V., Black, M., Vaughn, M. W., Dedhia, N., Richard McCombie, W., Lavine, K., Mittal, V., May, B., Kasschau, K. D., et al. (2004) · Nature · doi:10.1038/nature02651 · Cited by 1,065
Self-citationMultiple Forms of DNA-dependent RNA Polymerase in Eukaryotic Organisms
ROEDER, R. G., & RUTTER, W. J. (1969) · Nature · doi:10.1038/224234a0 · Cited by 970
Solubilization of Plant Membrane Proteins for Analysis by Two-Dimensional Gel Electrophoresis
Hurkman, W. J., & Tanaka, C. K. (1986) · Plant Physiology · doi:10.1104/pp.81.3.802 · Cited by 915
Structural Basis of Transcription: An RNA Polymerase II Elongation Complex at 3.3 Å Resolution
Gnatt, A. L., Cramer, P., Fu, J., Bushnell, D. A., & Kornberg, R. D. (2001) · Science · doi:10.1126/science.1059495 · Cited by 903
Cited by
Transposable elements and the epigenetic regulation of the genome
Slotkin, R. K., & Martienssen, R. (2007) · Nature Reviews Genetics · doi:10.1038/nrg2072 · Cited by 2,044
DNA methylation pathways and their crosstalk with histone methylation
Du, J., Johnson, L. M., Jacobsen, S. E., & Patel, D. J. (2015) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm4043 · Cited by 1,000
Epigenetic inheritance in plants
Henderson, I. R., & Jacobsen, S. E. (2007) · Nature · doi:10.1038/nature05917 · Cited by 835
Small RNA sorting: matchmaking for Argonautes
Czech, B., & Hannon, G. J. (2011) · Nature Reviews Genetics · doi:10.1038/nrg2916 · Cited by 605
Regulation and function of DNA methylation in plants and animals
He, X.-J., Chen, T., & Zhu, J.-K. (2011) · Cell Research · doi:10.1038/cr.2011.23 · Cited by 555
Metadata
- Authors
- Pontier, D., Yahubyan, G., Vega, D., Bulski, A., Saez-Vasquez, J., Hakimi, M.-A., Lerbs-Mache, S., Colot, V., & Lagrange, T.
- Venue
- Genes & Development
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 2005-09-01
- Volume
- 19
- Issue
- 17
- Pages
- 2030-2040
- ISSN
- 0890-9369 (ISSN-L)
- 1549-5477
- OpenAlex
- W2168780432
- Cited by
- 382
- References
- 40
- Type
- posted content
- Language
- en
- Open access
- diamond
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Reinforcement of silencing at transposons and highly repeated sequences requires the concerted action of two distinct RNA polymerases IV in Arabidopsis. https://app.cochat.ai/papers/10.1101/gad.348405
- The paper
- Pontier, D., Yahubyan, G., Vega, D., Bulski, A., Saez-Vasquez, J., Hakimi, M.-A., Lerbs-Mache, S., Colot, V., & Lagrange, T. (2005). Reinforcement of silencing at transposons and highly repeated sequences requires the concerted action of two distinct RNA polymerases IV in Arabidopsis. Genes & Development. https://doi.org/10.1101/gad.348405
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
